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Equilibrium density sucrose gradient <t>analysis</t> of virosomes. Schematic diagram of virosomes, showing the viral membrane lipids, viral proteins HA and NA, and the Amplivant-, or lipid conjugated peptide A . Virosomes were spun at 120.000 g for 64 h in a 10–60% sucrose gradient, showing a single band B with a density of around 1.1 g/ml. Fractions C were taken from bottom (left) to top and assayed for protein, lipid (phosphate) and sucrose concentration (converted to density) and by SDS-PAGE gel E , AV-T3756V is the Amplivant-conjugated SLP (left, fractions to the right), HA1 and HA2 are the subunits of influenza hemagglutinin. The size distribution of the virosomes D was measured by single <t>particle</t> <t>tracking</t> <t>sizing</t> analysis (Nanosight®)
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single-particle tracking nanosight analysis  (NanoSight ltd)
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Equilibrium density sucrose gradient <t>analysis</t> of virosomes. Schematic diagram of virosomes, showing the viral membrane lipids, viral proteins HA and NA, and the Amplivant-, or lipid conjugated peptide A . Virosomes were spun at 120.000 g for 64 h in a 10–60% sucrose gradient, showing a single band B with a density of around 1.1 g/ml. Fractions C were taken from bottom (left) to top and assayed for protein, lipid (phosphate) and sucrose concentration (converted to density) and by SDS-PAGE gel E , AV-T3756V is the Amplivant-conjugated SLP (left, fractions to the right), HA1 and HA2 are the subunits of influenza hemagglutinin. The size distribution of the virosomes D was measured by single <t>particle</t> <t>tracking</t> <t>sizing</t> analysis (Nanosight®)
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Equilibrium density sucrose gradient <t>analysis</t> of virosomes. Schematic diagram of virosomes, showing the viral membrane lipids, viral proteins HA and NA, and the Amplivant-, or lipid conjugated peptide A . Virosomes were spun at 120.000 g for 64 h in a 10–60% sucrose gradient, showing a single band B with a density of around 1.1 g/ml. Fractions C were taken from bottom (left) to top and assayed for protein, lipid (phosphate) and sucrose concentration (converted to density) and by SDS-PAGE gel E , AV-T3756V is the Amplivant-conjugated SLP (left, fractions to the right), HA1 and HA2 are the subunits of influenza hemagglutinin. The size distribution of the virosomes D was measured by single <t>particle</t> <t>tracking</t> <t>sizing</t> analysis (Nanosight®)
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Equilibrium density sucrose gradient <t>analysis</t> of virosomes. Schematic diagram of virosomes, showing the viral membrane lipids, viral proteins HA and NA, and the Amplivant-, or lipid conjugated peptide A . Virosomes were spun at 120.000 g for 64 h in a 10–60% sucrose gradient, showing a single band B with a density of around 1.1 g/ml. Fractions C were taken from bottom (left) to top and assayed for protein, lipid (phosphate) and sucrose concentration (converted to density) and by SDS-PAGE gel E , AV-T3756V is the Amplivant-conjugated SLP (left, fractions to the right), HA1 and HA2 are the subunits of influenza hemagglutinin. The size distribution of the virosomes D was measured by single <t>particle</t> <t>tracking</t> <t>sizing</t> analysis (Nanosight®)
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Equilibrium density sucrose gradient <t>analysis</t> of virosomes. Schematic diagram of virosomes, showing the viral membrane lipids, viral proteins HA and NA, and the Amplivant-, or lipid conjugated peptide A . Virosomes were spun at 120.000 g for 64 h in a 10–60% sucrose gradient, showing a single band B with a density of around 1.1 g/ml. Fractions C were taken from bottom (left) to top and assayed for protein, lipid (phosphate) and sucrose concentration (converted to density) and by SDS-PAGE gel E , AV-T3756V is the Amplivant-conjugated SLP (left, fractions to the right), HA1 and HA2 are the subunits of influenza hemagglutinin. The size distribution of the virosomes D was measured by single <t>particle</t> <t>tracking</t> <t>sizing</t> analysis (Nanosight®)
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Equilibrium density sucrose gradient analysis of virosomes. Schematic diagram of virosomes, showing the viral membrane lipids, viral proteins HA and NA, and the Amplivant-, or lipid conjugated peptide A . Virosomes were spun at 120.000 g for 64 h in a 10–60% sucrose gradient, showing a single band B with a density of around 1.1 g/ml. Fractions C were taken from bottom (left) to top and assayed for protein, lipid (phosphate) and sucrose concentration (converted to density) and by SDS-PAGE gel E , AV-T3756V is the Amplivant-conjugated SLP (left, fractions to the right), HA1 and HA2 are the subunits of influenza hemagglutinin. The size distribution of the virosomes D was measured by single particle tracking sizing analysis (Nanosight®)

Journal: Cancer Immunology, Immunotherapy

Article Title: Enhanced HPV16 E6/E7 + tumor eradication via induction of tumor-specific T cells by therapeutic vaccination with virosomes presenting synthetic long peptides

doi: 10.1007/s00262-023-03462-y

Figure Lengend Snippet: Equilibrium density sucrose gradient analysis of virosomes. Schematic diagram of virosomes, showing the viral membrane lipids, viral proteins HA and NA, and the Amplivant-, or lipid conjugated peptide A . Virosomes were spun at 120.000 g for 64 h in a 10–60% sucrose gradient, showing a single band B with a density of around 1.1 g/ml. Fractions C were taken from bottom (left) to top and assayed for protein, lipid (phosphate) and sucrose concentration (converted to density) and by SDS-PAGE gel E , AV-T3756V is the Amplivant-conjugated SLP (left, fractions to the right), HA1 and HA2 are the subunits of influenza hemagglutinin. The size distribution of the virosomes D was measured by single particle tracking sizing analysis (Nanosight®)

Article Snippet: The size distribution of the virosomes D was measured by single particle tracking sizing analysis (Nanosight®)

Techniques: Membrane, Concentration Assay, SDS Page, Single-particle Tracking