Journal: Cell reports

Article Title: Establishment of bovine extraembryonic endoderm stem cells enables efficient blastoid formation.

doi: 10.1016/j.celrep.2025.115707

Figure Lengend Snippet: Figure 2. Transcriptomic and chromatin-accessible features of bXENs (A) Principal-component analysis (PCA) of transcriptomes of bXEN, bEPSCemb, bEPSCiPS, and bTSC. (B) Heatmap showing the marker gene expression for PrE, VE, PE, Epi, and TE from each bovine stem cell type. (C) Heatmap (left panel) showing lineage-specific expressed genes for bXENs, bEPSCs, and bTSCs, as well as the enriched signaling pathways (right panel). (D) PCA of transcriptomes of bXENs and three major lineages from days 12 to 18 in vivo bovine embryos. (E) PCA of transcriptomes of XEN and ESC from bovine, mouse, and human. Datasets of bEPSCemb and bEPSCiPS are from Zhao et al., PNAS 202123; datasets of hNESCH1, hNESCH9, hNESCiPS, hPESC, hXEN7F, and hXENGATA6OE are from Okubo et al., Nature 202420; datasets of mESC, mpXEN and mXEN are from Zhong et al., Stem Cell Research 2018.26

Article Snippet: Green, SOX17; blue, SOX2; red, CDX2. (E) Blastoid formation rate from distinct protocols. (F) Blastocele diameter measurement of blastoids from distinct protocols and blastocysts. (G) Inner cell mass (ICM)/embryo ratio measurement of blastoids from distinct protocols and blastocysts. (H) Joint uniform manifold approximation and projection embedding of 10X Genomics single-nucleus transcriptomes of bovine EPTX-blastoids (green) and bovine day 8 blastocysts (blue). (I) Major cluster classification based on marker expression. (J) Dot plot indicating the expression of markers of epiblast, trophectoderm, and hypoblast. (K) Percentage of three cell types in blastoid and blastocyst.

Techniques: Marker, Gene Expression, Protein-Protein interactions, In Vivo