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A. The <t>consensus</t> <t>transcript</t> <t>expression</t> levels summarized per gene in 54 tissues in Human Protein Atlas (HPA), which was calculated as the maximum transcripts per million value (TPM) value for each gene in all sub-tissues categories of each tissue, based on transcriptomics data from HPA and GTEx. B. RNA single cell type tissue cluster data (transcript expression levels summarized per gene and cluster) of lung (GSE130148) and peripheral blood mononuclear cell (PBMC) (GSE112845) were visualized using log 10 (protein-transcripts per million [pTPM]) values. Each c-X annotation is taken from the clustering results performed in Human Protein Atlas. C. Single-cell RNA expression profile of 23 lung COVID-19 autopsy donor tissue samples from GSE171668 . The mean value of RNA expression of the cells annotated in the same subcategory was represented as a dot per each sample. The cell type annotation was manually performed in the original publication . AT1: alveolar type 1 epithelial cells, AT2: alveolar type 2 epithelial cells, EC: endothelial cells, KRT8 + PATS/ADI/DATPs: KRT8 + pre-alveolar type 1 transitional cell state, MAST: mast cells, XXhi: XX (gene expression) high cells, RBC: red blood cells.
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A. The consensus transcript expression levels summarized per gene in 54 tissues in Human Protein Atlas (HPA), which was calculated as the maximum transcripts per million value (TPM) value for each gene in all sub-tissues categories of each tissue, based on transcriptomics data from HPA and GTEx. B. RNA single cell type tissue cluster data (transcript expression levels summarized per gene and cluster) of lung (GSE130148) and peripheral blood mononuclear cell (PBMC) (GSE112845) were visualized using log 10 (protein-transcripts per million [pTPM]) values. Each c-X annotation is taken from the clustering results performed in Human Protein Atlas. C. Single-cell RNA expression profile of 23 lung COVID-19 autopsy donor tissue samples from GSE171668 . The mean value of RNA expression of the cells annotated in the same subcategory was represented as a dot per each sample. The cell type annotation was manually performed in the original publication . AT1: alveolar type 1 epithelial cells, AT2: alveolar type 2 epithelial cells, EC: endothelial cells, KRT8 + PATS/ADI/DATPs: KRT8 + pre-alveolar type 1 transitional cell state, MAST: mast cells, XXhi: XX (gene expression) high cells, RBC: red blood cells.

Journal: medRxiv

Article Title: Alternative splicing in the lung influences COVID-19 severity and respiratory diseases

doi: 10.1101/2022.10.18.22281202

Figure Lengend Snippet: A. The consensus transcript expression levels summarized per gene in 54 tissues in Human Protein Atlas (HPA), which was calculated as the maximum transcripts per million value (TPM) value for each gene in all sub-tissues categories of each tissue, based on transcriptomics data from HPA and GTEx. B. RNA single cell type tissue cluster data (transcript expression levels summarized per gene and cluster) of lung (GSE130148) and peripheral blood mononuclear cell (PBMC) (GSE112845) were visualized using log 10 (protein-transcripts per million [pTPM]) values. Each c-X annotation is taken from the clustering results performed in Human Protein Atlas. C. Single-cell RNA expression profile of 23 lung COVID-19 autopsy donor tissue samples from GSE171668 . The mean value of RNA expression of the cells annotated in the same subcategory was represented as a dot per each sample. The cell type annotation was manually performed in the original publication . AT1: alveolar type 1 epithelial cells, AT2: alveolar type 2 epithelial cells, EC: endothelial cells, KRT8 + PATS/ADI/DATPs: KRT8 + pre-alveolar type 1 transitional cell state, MAST: mast cells, XXhi: XX (gene expression) high cells, RBC: red blood cells.

Article Snippet: The consensus transcript expression levels and RNA single cell type tissue cluster data were downloadable from the Human Protein Atlas website ( www.proteinatlas.org/about/download ).

Techniques: Expressing, RNA Expression, Gene Expression