Review





Similar Products

low toxicity silicone adhesive  (World Precision Instruments)
96
World Precision Instruments low toxicity silicone adhesive
Low Toxicity Silicone Adhesive, supplied by World Precision Instruments, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/low toxicity silicone adhesive/product/World Precision Instruments
Average 96 stars, based on 1 article reviews
low toxicity silicone adhesive - by Bioz Stars, 2026-04
96/100 stars
  Buy from Supplier
sil  (MedChemExpress)
94
MedChemExpress sil
KLF15 knockdown canceled the effect of <t>SIL</t> on proliferation, migration, and ECM deposition in HSFbs. HSFbs were treated with TGF-β1 (5 ng/ml), SIL (100 µM) <t>and/or</t> <t>KT5823</t> (1 µM) for 24 h. (A) KLF15 mRNA expression was detected using RT-qPCR. (B) KLF15 protein levels were assessed using Western blotting assays. (C, D) HSFbs were treated with a negative control short hairpin (sh) RNA ( sh-NC) , or shRNAs targeting KLF15 ( sh-KLF15#1 or sh-KLF15#2 ) for 48 h. KLF15 mRNA expression was detected using RT-qPCR (C). KLF15 protein levels were examined using Western blotting assays (D). (E–H) HSFbs were treated with TGF-β1 (5 ng/ml), SIL (100 µM) co-incubated 5 ng/ml TGF-β1, SIL combined with a sh-NC and 5 ng/ml TGF-β1, or SIL combined with a KLF15- targeting shRNA and 5 ng/ml TGF-β1. (E) Cell viability was examined using CCK-8 assays. (F) Cell proliferation was detected using EdU assays. Scale bar = 100 µM. (G) Cell migration was assessed using transwell assays. Scale bar = 100 µM. (H) The expression of collagen I, fibronectin, and α-SMA was detected by Western blotting assays. Data are presented as the mean ± SD. All experiments were performed on three independent occasions. KLF15, Krüppel-like factor 15; SIL, sildenafil; ECM, extracellular matrix; HSFbs, hypertrophic scarring-derived fibroblasts; TGF-β1, transforming growth factor beta 1; CCK-8, Cell Counting Kit-8; EdU, 5-ethynyl-2′-deoxyuridine; α-SMA, alpha smooth muscle actin. *p < 0.05, **p < 0.01, ***p < 0.001.
Sil, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sil/product/MedChemExpress
Average 94 stars, based on 1 article reviews
sil - by Bioz Stars, 2026-04
94/100 stars
  Buy from Supplier
sils devices  (Covidien)
90
Covidien sils devices
KLF15 knockdown canceled the effect of <t>SIL</t> on proliferation, migration, and ECM deposition in HSFbs. HSFbs were treated with TGF-β1 (5 ng/ml), SIL (100 µM) <t>and/or</t> <t>KT5823</t> (1 µM) for 24 h. (A) KLF15 mRNA expression was detected using RT-qPCR. (B) KLF15 protein levels were assessed using Western blotting assays. (C, D) HSFbs were treated with a negative control short hairpin (sh) RNA ( sh-NC) , or shRNAs targeting KLF15 ( sh-KLF15#1 or sh-KLF15#2 ) for 48 h. KLF15 mRNA expression was detected using RT-qPCR (C). KLF15 protein levels were examined using Western blotting assays (D). (E–H) HSFbs were treated with TGF-β1 (5 ng/ml), SIL (100 µM) co-incubated 5 ng/ml TGF-β1, SIL combined with a sh-NC and 5 ng/ml TGF-β1, or SIL combined with a KLF15- targeting shRNA and 5 ng/ml TGF-β1. (E) Cell viability was examined using CCK-8 assays. (F) Cell proliferation was detected using EdU assays. Scale bar = 100 µM. (G) Cell migration was assessed using transwell assays. Scale bar = 100 µM. (H) The expression of collagen I, fibronectin, and α-SMA was detected by Western blotting assays. Data are presented as the mean ± SD. All experiments were performed on three independent occasions. KLF15, Krüppel-like factor 15; SIL, sildenafil; ECM, extracellular matrix; HSFbs, hypertrophic scarring-derived fibroblasts; TGF-β1, transforming growth factor beta 1; CCK-8, Cell Counting Kit-8; EdU, 5-ethynyl-2′-deoxyuridine; α-SMA, alpha smooth muscle actin. *p < 0.05, **p < 0.01, ***p < 0.001.
Sils Devices, supplied by Covidien, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sils devices/product/Covidien
Average 90 stars, based on 1 article reviews
sils devices - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
kwiksil  (World Precision Instruments)
96
World Precision Instruments kwiksil
KLF15 knockdown canceled the effect of <t>SIL</t> on proliferation, migration, and ECM deposition in HSFbs. HSFbs were treated with TGF-β1 (5 ng/ml), SIL (100 µM) <t>and/or</t> <t>KT5823</t> (1 µM) for 24 h. (A) KLF15 mRNA expression was detected using RT-qPCR. (B) KLF15 protein levels were assessed using Western blotting assays. (C, D) HSFbs were treated with a negative control short hairpin (sh) RNA ( sh-NC) , or shRNAs targeting KLF15 ( sh-KLF15#1 or sh-KLF15#2 ) for 48 h. KLF15 mRNA expression was detected using RT-qPCR (C). KLF15 protein levels were examined using Western blotting assays (D). (E–H) HSFbs were treated with TGF-β1 (5 ng/ml), SIL (100 µM) co-incubated 5 ng/ml TGF-β1, SIL combined with a sh-NC and 5 ng/ml TGF-β1, or SIL combined with a KLF15- targeting shRNA and 5 ng/ml TGF-β1. (E) Cell viability was examined using CCK-8 assays. (F) Cell proliferation was detected using EdU assays. Scale bar = 100 µM. (G) Cell migration was assessed using transwell assays. Scale bar = 100 µM. (H) The expression of collagen I, fibronectin, and α-SMA was detected by Western blotting assays. Data are presented as the mean ± SD. All experiments were performed on three independent occasions. KLF15, Krüppel-like factor 15; SIL, sildenafil; ECM, extracellular matrix; HSFbs, hypertrophic scarring-derived fibroblasts; TGF-β1, transforming growth factor beta 1; CCK-8, Cell Counting Kit-8; EdU, 5-ethynyl-2′-deoxyuridine; α-SMA, alpha smooth muscle actin. *p < 0.05, **p < 0.01, ***p < 0.001.
Kwiksil, supplied by World Precision Instruments, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/kwiksil/product/World Precision Instruments
Average 96 stars, based on 1 article reviews
kwiksil - by Bioz Stars, 2026-04
96/100 stars
  Buy from Supplier
spectrometer kymera 328i d2 sil  (Oxford Instruments)
97
Oxford Instruments spectrometer kymera 328i d2 sil
KLF15 knockdown canceled the effect of <t>SIL</t> on proliferation, migration, and ECM deposition in HSFbs. HSFbs were treated with TGF-β1 (5 ng/ml), SIL (100 µM) <t>and/or</t> <t>KT5823</t> (1 µM) for 24 h. (A) KLF15 mRNA expression was detected using RT-qPCR. (B) KLF15 protein levels were assessed using Western blotting assays. (C, D) HSFbs were treated with a negative control short hairpin (sh) RNA ( sh-NC) , or shRNAs targeting KLF15 ( sh-KLF15#1 or sh-KLF15#2 ) for 48 h. KLF15 mRNA expression was detected using RT-qPCR (C). KLF15 protein levels were examined using Western blotting assays (D). (E–H) HSFbs were treated with TGF-β1 (5 ng/ml), SIL (100 µM) co-incubated 5 ng/ml TGF-β1, SIL combined with a sh-NC and 5 ng/ml TGF-β1, or SIL combined with a KLF15- targeting shRNA and 5 ng/ml TGF-β1. (E) Cell viability was examined using CCK-8 assays. (F) Cell proliferation was detected using EdU assays. Scale bar = 100 µM. (G) Cell migration was assessed using transwell assays. Scale bar = 100 µM. (H) The expression of collagen I, fibronectin, and α-SMA was detected by Western blotting assays. Data are presented as the mean ± SD. All experiments were performed on three independent occasions. KLF15, Krüppel-like factor 15; SIL, sildenafil; ECM, extracellular matrix; HSFbs, hypertrophic scarring-derived fibroblasts; TGF-β1, transforming growth factor beta 1; CCK-8, Cell Counting Kit-8; EdU, 5-ethynyl-2′-deoxyuridine; α-SMA, alpha smooth muscle actin. *p < 0.05, **p < 0.01, ***p < 0.001.
Spectrometer Kymera 328i D2 Sil, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/spectrometer kymera 328i d2 sil/product/Oxford Instruments
Average 97 stars, based on 1 article reviews
spectrometer kymera 328i d2 sil - by Bioz Stars, 2026-04
97/100 stars
  Buy from Supplier
silicone adhesive  (World Precision Instruments)
96
World Precision Instruments silicone adhesive
KLF15 knockdown canceled the effect of <t>SIL</t> on proliferation, migration, and ECM deposition in HSFbs. HSFbs were treated with TGF-β1 (5 ng/ml), SIL (100 µM) <t>and/or</t> <t>KT5823</t> (1 µM) for 24 h. (A) KLF15 mRNA expression was detected using RT-qPCR. (B) KLF15 protein levels were assessed using Western blotting assays. (C, D) HSFbs were treated with a negative control short hairpin (sh) RNA ( sh-NC) , or shRNAs targeting KLF15 ( sh-KLF15#1 or sh-KLF15#2 ) for 48 h. KLF15 mRNA expression was detected using RT-qPCR (C). KLF15 protein levels were examined using Western blotting assays (D). (E–H) HSFbs were treated with TGF-β1 (5 ng/ml), SIL (100 µM) co-incubated 5 ng/ml TGF-β1, SIL combined with a sh-NC and 5 ng/ml TGF-β1, or SIL combined with a KLF15- targeting shRNA and 5 ng/ml TGF-β1. (E) Cell viability was examined using CCK-8 assays. (F) Cell proliferation was detected using EdU assays. Scale bar = 100 µM. (G) Cell migration was assessed using transwell assays. Scale bar = 100 µM. (H) The expression of collagen I, fibronectin, and α-SMA was detected by Western blotting assays. Data are presented as the mean ± SD. All experiments were performed on three independent occasions. KLF15, Krüppel-like factor 15; SIL, sildenafil; ECM, extracellular matrix; HSFbs, hypertrophic scarring-derived fibroblasts; TGF-β1, transforming growth factor beta 1; CCK-8, Cell Counting Kit-8; EdU, 5-ethynyl-2′-deoxyuridine; α-SMA, alpha smooth muscle actin. *p < 0.05, **p < 0.01, ***p < 0.001.
Silicone Adhesive, supplied by World Precision Instruments, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/silicone adhesive/product/World Precision Instruments
Average 96 stars, based on 1 article reviews
silicone adhesive - by Bioz Stars, 2026-04
96/100 stars
  Buy from Supplier
silicone elastomer  (World Precision Instruments)
96
World Precision Instruments silicone elastomer
KLF15 knockdown canceled the effect of <t>SIL</t> on proliferation, migration, and ECM deposition in HSFbs. HSFbs were treated with TGF-β1 (5 ng/ml), SIL (100 µM) <t>and/or</t> <t>KT5823</t> (1 µM) for 24 h. (A) KLF15 mRNA expression was detected using RT-qPCR. (B) KLF15 protein levels were assessed using Western blotting assays. (C, D) HSFbs were treated with a negative control short hairpin (sh) RNA ( sh-NC) , or shRNAs targeting KLF15 ( sh-KLF15#1 or sh-KLF15#2 ) for 48 h. KLF15 mRNA expression was detected using RT-qPCR (C). KLF15 protein levels were examined using Western blotting assays (D). (E–H) HSFbs were treated with TGF-β1 (5 ng/ml), SIL (100 µM) co-incubated 5 ng/ml TGF-β1, SIL combined with a sh-NC and 5 ng/ml TGF-β1, or SIL combined with a KLF15- targeting shRNA and 5 ng/ml TGF-β1. (E) Cell viability was examined using CCK-8 assays. (F) Cell proliferation was detected using EdU assays. Scale bar = 100 µM. (G) Cell migration was assessed using transwell assays. Scale bar = 100 µM. (H) The expression of collagen I, fibronectin, and α-SMA was detected by Western blotting assays. Data are presented as the mean ± SD. All experiments were performed on three independent occasions. KLF15, Krüppel-like factor 15; SIL, sildenafil; ECM, extracellular matrix; HSFbs, hypertrophic scarring-derived fibroblasts; TGF-β1, transforming growth factor beta 1; CCK-8, Cell Counting Kit-8; EdU, 5-ethynyl-2′-deoxyuridine; α-SMA, alpha smooth muscle actin. *p < 0.05, **p < 0.01, ***p < 0.001.
Silicone Elastomer, supplied by World Precision Instruments, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/silicone elastomer/product/World Precision Instruments
Average 96 stars, based on 1 article reviews
silicone elastomer - by Bioz Stars, 2026-04
96/100 stars
  Buy from Supplier

Image Search Results


KLF15 knockdown canceled the effect of SIL on proliferation, migration, and ECM deposition in HSFbs. HSFbs were treated with TGF-β1 (5 ng/ml), SIL (100 µM) and/or KT5823 (1 µM) for 24 h. (A) KLF15 mRNA expression was detected using RT-qPCR. (B) KLF15 protein levels were assessed using Western blotting assays. (C, D) HSFbs were treated with a negative control short hairpin (sh) RNA ( sh-NC) , or shRNAs targeting KLF15 ( sh-KLF15#1 or sh-KLF15#2 ) for 48 h. KLF15 mRNA expression was detected using RT-qPCR (C). KLF15 protein levels were examined using Western blotting assays (D). (E–H) HSFbs were treated with TGF-β1 (5 ng/ml), SIL (100 µM) co-incubated 5 ng/ml TGF-β1, SIL combined with a sh-NC and 5 ng/ml TGF-β1, or SIL combined with a KLF15- targeting shRNA and 5 ng/ml TGF-β1. (E) Cell viability was examined using CCK-8 assays. (F) Cell proliferation was detected using EdU assays. Scale bar = 100 µM. (G) Cell migration was assessed using transwell assays. Scale bar = 100 µM. (H) The expression of collagen I, fibronectin, and α-SMA was detected by Western blotting assays. Data are presented as the mean ± SD. All experiments were performed on three independent occasions. KLF15, Krüppel-like factor 15; SIL, sildenafil; ECM, extracellular matrix; HSFbs, hypertrophic scarring-derived fibroblasts; TGF-β1, transforming growth factor beta 1; CCK-8, Cell Counting Kit-8; EdU, 5-ethynyl-2′-deoxyuridine; α-SMA, alpha smooth muscle actin. *p < 0.05, **p < 0.01, ***p < 0.001.

Journal: The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology

Article Title: Sildenafil suppresses the activation of hypertrophic scar fibroblasts by promoting the KLF15-mediated inhibition of LOXL1 transcription

doi: 10.4196/kjpp.25.179

Figure Lengend Snippet: KLF15 knockdown canceled the effect of SIL on proliferation, migration, and ECM deposition in HSFbs. HSFbs were treated with TGF-β1 (5 ng/ml), SIL (100 µM) and/or KT5823 (1 µM) for 24 h. (A) KLF15 mRNA expression was detected using RT-qPCR. (B) KLF15 protein levels were assessed using Western blotting assays. (C, D) HSFbs were treated with a negative control short hairpin (sh) RNA ( sh-NC) , or shRNAs targeting KLF15 ( sh-KLF15#1 or sh-KLF15#2 ) for 48 h. KLF15 mRNA expression was detected using RT-qPCR (C). KLF15 protein levels were examined using Western blotting assays (D). (E–H) HSFbs were treated with TGF-β1 (5 ng/ml), SIL (100 µM) co-incubated 5 ng/ml TGF-β1, SIL combined with a sh-NC and 5 ng/ml TGF-β1, or SIL combined with a KLF15- targeting shRNA and 5 ng/ml TGF-β1. (E) Cell viability was examined using CCK-8 assays. (F) Cell proliferation was detected using EdU assays. Scale bar = 100 µM. (G) Cell migration was assessed using transwell assays. Scale bar = 100 µM. (H) The expression of collagen I, fibronectin, and α-SMA was detected by Western blotting assays. Data are presented as the mean ± SD. All experiments were performed on three independent occasions. KLF15, Krüppel-like factor 15; SIL, sildenafil; ECM, extracellular matrix; HSFbs, hypertrophic scarring-derived fibroblasts; TGF-β1, transforming growth factor beta 1; CCK-8, Cell Counting Kit-8; EdU, 5-ethynyl-2′-deoxyuridine; α-SMA, alpha smooth muscle actin. *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: The cells were treated with 5 ng/ml TGF-β1 (100-21C; Peprotech) for 24 h. According to experimental groups, cells were co-treated with 10, 20, 50, and 100 μM SIL (HY-15025; MedChemExpress) and/or 1 μM KT5823 (10010965; Cayman).

Techniques: Knockdown, Migration, Expressing, Quantitative RT-PCR, Western Blot, Negative Control, Incubation, shRNA, CCK-8 Assay, Derivative Assay, Cell Counting