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Sartorius AG si rna targeting
Si Rna Targeting, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Results of functional validation experiments on target genes AGPAT3 and ECHDC3 in adipocytes (A) qPCR results of expression levels of 16 lipid metabolism-related genes. mRNA expression of the target genes was significantly knocked down by approximately 90% in all three <t>transfection</t> groups. The expression of FABP4 , FASN , ADIPOR1 , and CEBPα was significantly elevated in the si- AGPAT3 group, especially FABP4 and ADIPOR1 (∗ p < 0.05 by Student’s t test). (B) Comparison results of the triglyceride content between the two target gene knockdown groups and the control group. Triglyceride quantification revealed that triglyceride levels were significantly higher in all three knockdown groups than in the control group (∗ p < 0.05 by Dunnett’s test). (C–F) Comparison results of four fatty acids content (C18:0, C18:2, C22:0, and C22:6) between the two target gene knockdown groups and the control group. Fatty acid profiling showed that both AGPAT3 and ECHDC3 knockdown significantly suppressed the accumulation of most fatty acids, including C18:0, C18:2, and C22:6 (∗ p < 0.05 by Dunnett’s test). Four groups were included: si-NC (negative control, white), si- AGPAT3 (red), si- ECHDC3 (green), and si-A3+si-E3 (double knockdown, blue) ( n = 3 technical replicates from 4 biological replicates for each transfection group). Data are represented as the mean ± SEM.
Si Rna Transfection, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Results of functional validation experiments on target genes AGPAT3 and ECHDC3 in adipocytes (A) qPCR results of expression levels of 16 lipid metabolism-related genes. mRNA expression of the target genes was significantly knocked down by approximately 90% in all three <t>transfection</t> groups. The expression of FABP4 , FASN , ADIPOR1 , and CEBPα was significantly elevated in the si- AGPAT3 group, especially FABP4 and ADIPOR1 (∗ p < 0.05 by Student’s t test). (B) Comparison results of the triglyceride content between the two target gene knockdown groups and the control group. Triglyceride quantification revealed that triglyceride levels were significantly higher in all three knockdown groups than in the control group (∗ p < 0.05 by Dunnett’s test). (C–F) Comparison results of four fatty acids content (C18:0, C18:2, C22:0, and C22:6) between the two target gene knockdown groups and the control group. Fatty acid profiling showed that both AGPAT3 and ECHDC3 knockdown significantly suppressed the accumulation of most fatty acids, including C18:0, C18:2, and C22:6 (∗ p < 0.05 by Dunnett’s test). Four groups were included: si-NC (negative control, white), si- AGPAT3 (red), si- ECHDC3 (green), and si-A3+si-E3 (double knockdown, blue) ( n = 3 technical replicates from 4 biological replicates for each transfection group). Data are represented as the mean ± SEM.
Si Rna, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Results of functional validation experiments on target genes AGPAT3 and ECHDC3 in adipocytes (A) qPCR results of expression levels of 16 lipid metabolism-related genes. mRNA expression of the target genes was significantly knocked down by approximately 90% in all three <t>transfection</t> groups. The expression of FABP4 , FASN , ADIPOR1 , and CEBPα was significantly elevated in the si- AGPAT3 group, especially FABP4 and ADIPOR1 (∗ p < 0.05 by Student’s t test). (B) Comparison results of the triglyceride content between the two target gene knockdown groups and the control group. Triglyceride quantification revealed that triglyceride levels were significantly higher in all three knockdown groups than in the control group (∗ p < 0.05 by Dunnett’s test). (C–F) Comparison results of four fatty acids content (C18:0, C18:2, C22:0, and C22:6) between the two target gene knockdown groups and the control group. Fatty acid profiling showed that both AGPAT3 and ECHDC3 knockdown significantly suppressed the accumulation of most fatty acids, including C18:0, C18:2, and C22:6 (∗ p < 0.05 by Dunnett’s test). Four groups were included: si-NC (negative control, white), si- AGPAT3 (red), si- ECHDC3 (green), and si-A3+si-E3 (double knockdown, blue) ( n = 3 technical replicates from 4 biological replicates for each transfection group). Data are represented as the mean ± SEM.
Pin1 Si Rna, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Sangon Biotech si rna forward 5 ccaaugucuggugacuuutt 3 and reverse 5 aaagucaccagacauuuggtt 3
Results of functional validation experiments on target genes AGPAT3 and ECHDC3 in adipocytes (A) qPCR results of expression levels of 16 lipid metabolism-related genes. mRNA expression of the target genes was significantly knocked down by approximately 90% in all three <t>transfection</t> groups. The expression of FABP4 , FASN , ADIPOR1 , and CEBPα was significantly elevated in the si- AGPAT3 group, especially FABP4 and ADIPOR1 (∗ p < 0.05 by Student’s t test). (B) Comparison results of the triglyceride content between the two target gene knockdown groups and the control group. Triglyceride quantification revealed that triglyceride levels were significantly higher in all three knockdown groups than in the control group (∗ p < 0.05 by Dunnett’s test). (C–F) Comparison results of four fatty acids content (C18:0, C18:2, C22:0, and C22:6) between the two target gene knockdown groups and the control group. Fatty acid profiling showed that both AGPAT3 and ECHDC3 knockdown significantly suppressed the accumulation of most fatty acids, including C18:0, C18:2, and C22:6 (∗ p < 0.05 by Dunnett’s test). Four groups were included: si-NC (negative control, white), si- AGPAT3 (red), si- ECHDC3 (green), and si-A3+si-E3 (double knockdown, blue) ( n = 3 technical replicates from 4 biological replicates for each transfection group). Data are represented as the mean ± SEM.
Si Rna Forward 5 Ccaaugucuggugacuuutt 3 And Reverse 5 Aaagucaccagacauuuggtt 3, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Results of functional validation experiments on target genes AGPAT3 and ECHDC3 in adipocytes (A) qPCR results of expression levels of 16 lipid metabolism-related genes. mRNA expression of the target genes was significantly knocked down by approximately 90% in all three <t>transfection</t> groups. The expression of FABP4 , FASN , ADIPOR1 , and CEBPα was significantly elevated in the si- AGPAT3 group, especially FABP4 and ADIPOR1 (∗ p < 0.05 by Student’s t test). (B) Comparison results of the triglyceride content between the two target gene knockdown groups and the control group. Triglyceride quantification revealed that triglyceride levels were significantly higher in all three knockdown groups than in the control group (∗ p < 0.05 by Dunnett’s test). (C–F) Comparison results of four fatty acids content (C18:0, C18:2, C22:0, and C22:6) between the two target gene knockdown groups and the control group. Fatty acid profiling showed that both AGPAT3 and ECHDC3 knockdown significantly suppressed the accumulation of most fatty acids, including C18:0, C18:2, and C22:6 (∗ p < 0.05 by Dunnett’s test). Four groups were included: si-NC (negative control, white), si- AGPAT3 (red), si- ECHDC3 (green), and si-A3+si-E3 (double knockdown, blue) ( n = 3 technical replicates from 4 biological replicates for each transfection group). Data are represented as the mean ± SEM.
Si Rna Mate, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Sangon Biotech mtdh silencing rna si mtdh
Results of functional validation experiments on target genes AGPAT3 and ECHDC3 in adipocytes (A) qPCR results of expression levels of 16 lipid metabolism-related genes. mRNA expression of the target genes was significantly knocked down by approximately 90% in all three <t>transfection</t> groups. The expression of FABP4 , FASN , ADIPOR1 , and CEBPα was significantly elevated in the si- AGPAT3 group, especially FABP4 and ADIPOR1 (∗ p < 0.05 by Student’s t test). (B) Comparison results of the triglyceride content between the two target gene knockdown groups and the control group. Triglyceride quantification revealed that triglyceride levels were significantly higher in all three knockdown groups than in the control group (∗ p < 0.05 by Dunnett’s test). (C–F) Comparison results of four fatty acids content (C18:0, C18:2, C22:0, and C22:6) between the two target gene knockdown groups and the control group. Fatty acid profiling showed that both AGPAT3 and ECHDC3 knockdown significantly suppressed the accumulation of most fatty acids, including C18:0, C18:2, and C22:6 (∗ p < 0.05 by Dunnett’s test). Four groups were included: si-NC (negative control, white), si- AGPAT3 (red), si- ECHDC3 (green), and si-A3+si-E3 (double knockdown, blue) ( n = 3 technical replicates from 4 biological replicates for each transfection group). Data are represented as the mean ± SEM.
Mtdh Silencing Rna Si Mtdh, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Results of functional validation experiments on target genes AGPAT3 and ECHDC3 in adipocytes (A) qPCR results of expression levels of 16 lipid metabolism-related genes. mRNA expression of the target genes was significantly knocked down by approximately 90% in all three <t>transfection</t> groups. The expression of FABP4 , FASN , ADIPOR1 , and CEBPα was significantly elevated in the si- AGPAT3 group, especially FABP4 and ADIPOR1 (∗ p < 0.05 by Student’s t test). (B) Comparison results of the triglyceride content between the two target gene knockdown groups and the control group. Triglyceride quantification revealed that triglyceride levels were significantly higher in all three knockdown groups than in the control group (∗ p < 0.05 by Dunnett’s test). (C–F) Comparison results of four fatty acids content (C18:0, C18:2, C22:0, and C22:6) between the two target gene knockdown groups and the control group. Fatty acid profiling showed that both AGPAT3 and ECHDC3 knockdown significantly suppressed the accumulation of most fatty acids, including C18:0, C18:2, and C22:6 (∗ p < 0.05 by Dunnett’s test). Four groups were included: si-NC (negative control, white), si- AGPAT3 (red), si- ECHDC3 (green), and si-A3+si-E3 (double knockdown, blue) ( n = 3 technical replicates from 4 biological replicates for each transfection group). Data are represented as the mean ± SEM.
Si Rna Forward 5 Cca Aug Ucu Ggu Gac Uuu Tt 3 And Reverse 5 Aaa Guc Acc Aga Cau Uug Gtt 3, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Santa Cruz Biotechnology si rna against p53
Results of functional validation experiments on target genes AGPAT3 and ECHDC3 in adipocytes (A) qPCR results of expression levels of 16 lipid metabolism-related genes. mRNA expression of the target genes was significantly knocked down by approximately 90% in all three <t>transfection</t> groups. The expression of FABP4 , FASN , ADIPOR1 , and CEBPα was significantly elevated in the si- AGPAT3 group, especially FABP4 and ADIPOR1 (∗ p < 0.05 by Student’s t test). (B) Comparison results of the triglyceride content between the two target gene knockdown groups and the control group. Triglyceride quantification revealed that triglyceride levels were significantly higher in all three knockdown groups than in the control group (∗ p < 0.05 by Dunnett’s test). (C–F) Comparison results of four fatty acids content (C18:0, C18:2, C22:0, and C22:6) between the two target gene knockdown groups and the control group. Fatty acid profiling showed that both AGPAT3 and ECHDC3 knockdown significantly suppressed the accumulation of most fatty acids, including C18:0, C18:2, and C22:6 (∗ p < 0.05 by Dunnett’s test). Four groups were included: si-NC (negative control, white), si- AGPAT3 (red), si- ECHDC3 (green), and si-A3+si-E3 (double knockdown, blue) ( n = 3 technical replicates from 4 biological replicates for each transfection group). Data are represented as the mean ± SEM.
Si Rna Against P53, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Shanghai Genechem Ltd si rna sicd36 1 sicd36 2 and sicd36 3
Results of functional validation experiments on target genes AGPAT3 and ECHDC3 in adipocytes (A) qPCR results of expression levels of 16 lipid metabolism-related genes. mRNA expression of the target genes was significantly knocked down by approximately 90% in all three <t>transfection</t> groups. The expression of FABP4 , FASN , ADIPOR1 , and CEBPα was significantly elevated in the si- AGPAT3 group, especially FABP4 and ADIPOR1 (∗ p < 0.05 by Student’s t test). (B) Comparison results of the triglyceride content between the two target gene knockdown groups and the control group. Triglyceride quantification revealed that triglyceride levels were significantly higher in all three knockdown groups than in the control group (∗ p < 0.05 by Dunnett’s test). (C–F) Comparison results of four fatty acids content (C18:0, C18:2, C22:0, and C22:6) between the two target gene knockdown groups and the control group. Fatty acid profiling showed that both AGPAT3 and ECHDC3 knockdown significantly suppressed the accumulation of most fatty acids, including C18:0, C18:2, and C22:6 (∗ p < 0.05 by Dunnett’s test). Four groups were included: si-NC (negative control, white), si- AGPAT3 (red), si- ECHDC3 (green), and si-A3+si-E3 (double knockdown, blue) ( n = 3 technical replicates from 4 biological replicates for each transfection group). Data are represented as the mean ± SEM.
Si Rna Sicd36 1 Sicd36 2 And Sicd36 3, supplied by Shanghai Genechem Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Results of functional validation experiments on target genes AGPAT3 and ECHDC3 in adipocytes (A) qPCR results of expression levels of 16 lipid metabolism-related genes. mRNA expression of the target genes was significantly knocked down by approximately 90% in all three transfection groups. The expression of FABP4 , FASN , ADIPOR1 , and CEBPα was significantly elevated in the si- AGPAT3 group, especially FABP4 and ADIPOR1 (∗ p < 0.05 by Student’s t test). (B) Comparison results of the triglyceride content between the two target gene knockdown groups and the control group. Triglyceride quantification revealed that triglyceride levels were significantly higher in all three knockdown groups than in the control group (∗ p < 0.05 by Dunnett’s test). (C–F) Comparison results of four fatty acids content (C18:0, C18:2, C22:0, and C22:6) between the two target gene knockdown groups and the control group. Fatty acid profiling showed that both AGPAT3 and ECHDC3 knockdown significantly suppressed the accumulation of most fatty acids, including C18:0, C18:2, and C22:6 (∗ p < 0.05 by Dunnett’s test). Four groups were included: si-NC (negative control, white), si- AGPAT3 (red), si- ECHDC3 (green), and si-A3+si-E3 (double knockdown, blue) ( n = 3 technical replicates from 4 biological replicates for each transfection group). Data are represented as the mean ± SEM.

Journal: iScience

Article Title: Integration of GWAS and eQTL analysis reveals regulatory CNVs for fatty acid traits in cattle

doi: 10.1016/j.isci.2026.116180

Figure Lengend Snippet: Results of functional validation experiments on target genes AGPAT3 and ECHDC3 in adipocytes (A) qPCR results of expression levels of 16 lipid metabolism-related genes. mRNA expression of the target genes was significantly knocked down by approximately 90% in all three transfection groups. The expression of FABP4 , FASN , ADIPOR1 , and CEBPα was significantly elevated in the si- AGPAT3 group, especially FABP4 and ADIPOR1 (∗ p < 0.05 by Student’s t test). (B) Comparison results of the triglyceride content between the two target gene knockdown groups and the control group. Triglyceride quantification revealed that triglyceride levels were significantly higher in all three knockdown groups than in the control group (∗ p < 0.05 by Dunnett’s test). (C–F) Comparison results of four fatty acids content (C18:0, C18:2, C22:0, and C22:6) between the two target gene knockdown groups and the control group. Fatty acid profiling showed that both AGPAT3 and ECHDC3 knockdown significantly suppressed the accumulation of most fatty acids, including C18:0, C18:2, and C22:6 (∗ p < 0.05 by Dunnett’s test). Four groups were included: si-NC (negative control, white), si- AGPAT3 (red), si- ECHDC3 (green), and si-A3+si-E3 (double knockdown, blue) ( n = 3 technical replicates from 4 biological replicates for each transfection group). Data are represented as the mean ± SEM.

Article Snippet: The si-RNA transfection reagents were purchased from Sangon Biotech (Shanghai) Co., Ltd.

Techniques: Functional Assay, Biomarker Discovery, Expressing, Transfection, Comparison, Knockdown, Control, Negative Control