Journal: Animals : an open access journal from MDPI

Article Title: Comparative Analysis of Proteomic Characteristics in Seminal Plasma Between Horses and Donkeys.

doi: 10.3390/ani15111532

Figure Lengend Snippet: Figure 5. Classification of seminal plasma SEPs in horses and donkeys. (a) A total of 59 SEPs in horse seminal plasma, with the different-sized circles indicating the expression levels of proteins. (b) The expression levels of the top 10 SEPs in horse seminal plasma. (c) GPX5 protein expression in horse and donkey seminal plasma was determined by Western blotting. (d) Quantification of the relative intensity of GPX5/TUBB. (e) A total of 64 SEPs in donkey seminal plasma, with the different- sized circles indicating the expression levels of proteins. (f) Expression levels of the top 10 SEPs in donkey seminal plasma. (g) SERPINE2 protein expression in horse and donkey seminal plasma was determined by Western blotting. (h) Quantification of the relative intensity of SERPINE2/TUBB. TUBB protein expression was employed as an internal reference for normalization. p < 0.05 indicates a statistically significant difference.

Article Snippet: The membrane was incubated overnight at 4 ◦C in blocking solution containing primary antibodies against GPX5 (1:500; 18731-1-AP, Proteintech, Rosemont, IL, USA), SERPINE2 (1:500; 66203-1-Ig, Proteintech), FOLR2 (1:500; 31264-1-AP, Proteintech), ACACA (1:500; 21923-1-AP, Proteintech), and tubulin (1:1000; ab6046, Abcam, Cambridge, MA, USA).

Techniques: Clinical Proteomics, Expressing, Western Blot