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In vivo (A–E) and in vitro (F–J) effects of rTMS on METTL3, m6A methylation, <t>NMDAR2B,</t> and YTHDF1. (A) METTL3, NMDAR2B, YTHDF1 protein bands in the DLPFC. (B–D) Western blot analysis of METTL3, YTHDF1, and NMDAR2B protein expression in the DLPFC. (E) ELISA analysis of the methylation modification level of m6A in the DLPFC. The number of samples in each group is 3. (F) METTL3, NMDAR2B, YTHDF1 protein bands in BV2 cells. (G–J) Western blot analysis of METTL3, YTHDF1, and NMDAR2B protein expression levels in BV2 cells. (P) ELISA analysis of the methylation modification level of m6A in BV2 cells. The number of samples in each group was 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.
Nmdar2b, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rat rac alpha serine  (Shanghai Korain Biotech Co Ltd)
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In vivo (A–E) and in vitro (F–J) effects of rTMS on METTL3, m6A methylation, <t>NMDAR2B,</t> and YTHDF1. (A) METTL3, NMDAR2B, YTHDF1 protein bands in the DLPFC. (B–D) Western blot analysis of METTL3, YTHDF1, and NMDAR2B protein expression in the DLPFC. (E) ELISA analysis of the methylation modification level of m6A in the DLPFC. The number of samples in each group is 3. (F) METTL3, NMDAR2B, YTHDF1 protein bands in BV2 cells. (G–J) Western blot analysis of METTL3, YTHDF1, and NMDAR2B protein expression levels in BV2 cells. (P) ELISA analysis of the methylation modification level of m6A in BV2 cells. The number of samples in each group was 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.
Rat Rac Alpha Serine, supplied by Shanghai Korain Biotech Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti receptor interacting serine threonine protein kinase 3  (Proteintech)
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In vivo (A–E) and in vitro (F–J) effects of rTMS on METTL3, m6A methylation, <t>NMDAR2B,</t> and YTHDF1. (A) METTL3, NMDAR2B, YTHDF1 protein bands in the DLPFC. (B–D) Western blot analysis of METTL3, YTHDF1, and NMDAR2B protein expression in the DLPFC. (E) ELISA analysis of the methylation modification level of m6A in the DLPFC. The number of samples in each group is 3. (F) METTL3, NMDAR2B, YTHDF1 protein bands in BV2 cells. (G–J) Western blot analysis of METTL3, YTHDF1, and NMDAR2B protein expression levels in BV2 cells. (P) ELISA analysis of the methylation modification level of m6A in BV2 cells. The number of samples in each group was 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.
Rabbit Anti Receptor Interacting Serine Threonine Protein Kinase 3, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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d-serine  (MedChemExpress)
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In vivo (A–E) and in vitro (F–J) effects of rTMS on METTL3, m6A methylation, <t>NMDAR2B,</t> and YTHDF1. (A) METTL3, NMDAR2B, YTHDF1 protein bands in the DLPFC. (B–D) Western blot analysis of METTL3, YTHDF1, and NMDAR2B protein expression in the DLPFC. (E) ELISA analysis of the methylation modification level of m6A in the DLPFC. The number of samples in each group is 3. (F) METTL3, NMDAR2B, YTHDF1 protein bands in BV2 cells. (G–J) Western blot analysis of METTL3, YTHDF1, and NMDAR2B protein expression levels in BV2 cells. (P) ELISA analysis of the methylation modification level of m6A in BV2 cells. The number of samples in each group was 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.
D Serine, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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threonine protein kinase akt1 elisa kit  (Shanghai Korain Biotech Co Ltd)
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In vivo (A–E) and in vitro (F–J) effects of rTMS on METTL3, m6A methylation, <t>NMDAR2B,</t> and YTHDF1. (A) METTL3, NMDAR2B, YTHDF1 protein bands in the DLPFC. (B–D) Western blot analysis of METTL3, YTHDF1, and NMDAR2B protein expression in the DLPFC. (E) ELISA analysis of the methylation modification level of m6A in the DLPFC. The number of samples in each group is 3. (F) METTL3, NMDAR2B, YTHDF1 protein bands in BV2 cells. (G–J) Western blot analysis of METTL3, YTHDF1, and NMDAR2B protein expression levels in BV2 cells. (P) ELISA analysis of the methylation modification level of m6A in BV2 cells. The number of samples in each group was 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.
Threonine Protein Kinase Akt1 Elisa Kit, supplied by Shanghai Korain Biotech Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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In vivo (A–E) and in vitro (F–J) effects of rTMS on METTL3, m6A methylation, <t>NMDAR2B,</t> and YTHDF1. (A) METTL3, NMDAR2B, YTHDF1 protein bands in the DLPFC. (B–D) Western blot analysis of METTL3, YTHDF1, and NMDAR2B protein expression in the DLPFC. (E) ELISA analysis of the methylation modification level of m6A in the DLPFC. The number of samples in each group is 3. (F) METTL3, NMDAR2B, YTHDF1 protein bands in BV2 cells. (G–J) Western blot analysis of METTL3, YTHDF1, and NMDAR2B protein expression levels in BV2 cells. (P) ELISA analysis of the methylation modification level of m6A in BV2 cells. The number of samples in each group was 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.
Anti Phospho Serine Rabbit Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti phospho serine polyclonal antibody  (Bioss)
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In vivo (A–E) and in vitro (F–J) effects of rTMS on METTL3, m6A methylation, <t>NMDAR2B,</t> and YTHDF1. (A) METTL3, NMDAR2B, YTHDF1 protein bands in the DLPFC. (B–D) Western blot analysis of METTL3, YTHDF1, and NMDAR2B protein expression in the DLPFC. (E) ELISA analysis of the methylation modification level of m6A in the DLPFC. The number of samples in each group is 3. (F) METTL3, NMDAR2B, YTHDF1 protein bands in BV2 cells. (G–J) Western blot analysis of METTL3, YTHDF1, and NMDAR2B protein expression levels in BV2 cells. (P) ELISA analysis of the methylation modification level of m6A in BV2 cells. The number of samples in each group was 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.
Rabbit Anti Phospho Serine Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1 palmitoyl 2 oleoyl sn glycero 3 phospho l serine pops  (Croda International Plc)
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Croda International Plc 1 palmitoyl 2 oleoyl sn glycero 3 phospho l serine pops
In vivo (A–E) and in vitro (F–J) effects of rTMS on METTL3, m6A methylation, <t>NMDAR2B,</t> and YTHDF1. (A) METTL3, NMDAR2B, YTHDF1 protein bands in the DLPFC. (B–D) Western blot analysis of METTL3, YTHDF1, and NMDAR2B protein expression in the DLPFC. (E) ELISA analysis of the methylation modification level of m6A in the DLPFC. The number of samples in each group is 3. (F) METTL3, NMDAR2B, YTHDF1 protein bands in BV2 cells. (G–J) Western blot analysis of METTL3, YTHDF1, and NMDAR2B protein expression levels in BV2 cells. (P) ELISA analysis of the methylation modification level of m6A in BV2 cells. The number of samples in each group was 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.
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1 2 dioleoyl sn glycero 3 phospho l serine dops  (Croda International Plc)
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In vivo (A–E) and in vitro (F–J) effects of rTMS on METTL3, m6A methylation, <t>NMDAR2B,</t> and YTHDF1. (A) METTL3, NMDAR2B, YTHDF1 protein bands in the DLPFC. (B–D) Western blot analysis of METTL3, YTHDF1, and NMDAR2B protein expression in the DLPFC. (E) ELISA analysis of the methylation modification level of m6A in the DLPFC. The number of samples in each group is 3. (F) METTL3, NMDAR2B, YTHDF1 protein bands in BV2 cells. (G–J) Western blot analysis of METTL3, YTHDF1, and NMDAR2B protein expression levels in BV2 cells. (P) ELISA analysis of the methylation modification level of m6A in BV2 cells. The number of samples in each group was 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.
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In vivo (A–E) and in vitro (F–J) effects of rTMS on METTL3, m6A methylation, NMDAR2B, and YTHDF1. (A) METTL3, NMDAR2B, YTHDF1 protein bands in the DLPFC. (B–D) Western blot analysis of METTL3, YTHDF1, and NMDAR2B protein expression in the DLPFC. (E) ELISA analysis of the methylation modification level of m6A in the DLPFC. The number of samples in each group is 3. (F) METTL3, NMDAR2B, YTHDF1 protein bands in BV2 cells. (G–J) Western blot analysis of METTL3, YTHDF1, and NMDAR2B protein expression levels in BV2 cells. (P) ELISA analysis of the methylation modification level of m6A in BV2 cells. The number of samples in each group was 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.

Journal: Frontiers in Immunology

Article Title: Repetitive transcranial magnetic stimulation alleviates neuropathic pain via microglial polarization by modulating the METTL3/NMDAR2B/NLRP3 pathway

doi: 10.3389/fimmu.2025.1666920

Figure Lengend Snippet: In vivo (A–E) and in vitro (F–J) effects of rTMS on METTL3, m6A methylation, NMDAR2B, and YTHDF1. (A) METTL3, NMDAR2B, YTHDF1 protein bands in the DLPFC. (B–D) Western blot analysis of METTL3, YTHDF1, and NMDAR2B protein expression in the DLPFC. (E) ELISA analysis of the methylation modification level of m6A in the DLPFC. The number of samples in each group is 3. (F) METTL3, NMDAR2B, YTHDF1 protein bands in BV2 cells. (G–J) Western blot analysis of METTL3, YTHDF1, and NMDAR2B protein expression levels in BV2 cells. (P) ELISA analysis of the methylation modification level of m6A in BV2 cells. The number of samples in each group was 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.

Article Snippet: After seeding, BV2 cells were cultured in complete DMEM for 24 h. Subsequently, specific pharmacological activation and inhibition targeting METTL3 (METTL3 activator-1; HY-W037893; MedChemExpress; STM2457; T9060; Targetmol) and NMDAR2B ( d -Serine; HY-100808; MedChemExpress; D-AP5; HY-100714A; MedChemExpress) were added to the culture medium, and their effects were verified.

Techniques: In Vivo, In Vitro, Methylation, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay, Modification

The roles of METTL3 and NMDAR2B in the anti-inflammatory effects of magnetic stimulation in vitro . (A) Effects of inhibition and overexpression of METTL3 on the protein bands of METTL3, NMDAR2B, and YTHDF1. (B–D) Western blot analyses of METTL3, YTHDF1, and NMDAR2B protein expression. (E) Effects of inhibition and overexpression of NMDAR2B on the protein bands of METTL3, NMDAR2B, and YTHDF1 (F–H) Western blot analyses of METTL3, YTHDF1, and NMDAR2B protein expression. (I–R) ELISA was used to detect the methylation modification level of m6A, NLRP3, IL-6, TNF-α, and IL-10. The number of samples in each group was 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.

Journal: Frontiers in Immunology

Article Title: Repetitive transcranial magnetic stimulation alleviates neuropathic pain via microglial polarization by modulating the METTL3/NMDAR2B/NLRP3 pathway

doi: 10.3389/fimmu.2025.1666920

Figure Lengend Snippet: The roles of METTL3 and NMDAR2B in the anti-inflammatory effects of magnetic stimulation in vitro . (A) Effects of inhibition and overexpression of METTL3 on the protein bands of METTL3, NMDAR2B, and YTHDF1. (B–D) Western blot analyses of METTL3, YTHDF1, and NMDAR2B protein expression. (E) Effects of inhibition and overexpression of NMDAR2B on the protein bands of METTL3, NMDAR2B, and YTHDF1 (F–H) Western blot analyses of METTL3, YTHDF1, and NMDAR2B protein expression. (I–R) ELISA was used to detect the methylation modification level of m6A, NLRP3, IL-6, TNF-α, and IL-10. The number of samples in each group was 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.

Article Snippet: After seeding, BV2 cells were cultured in complete DMEM for 24 h. Subsequently, specific pharmacological activation and inhibition targeting METTL3 (METTL3 activator-1; HY-W037893; MedChemExpress; STM2457; T9060; Targetmol) and NMDAR2B ( d -Serine; HY-100808; MedChemExpress; D-AP5; HY-100714A; MedChemExpress) were added to the culture medium, and their effects were verified.

Techniques: In Vitro, Inhibition, Over Expression, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay, Methylation, Modification

The roles of METTL3 and NMDAR2B in the regulation of microglial cell polarization by magnetic stimulation in vitro . (A–C, G–I) Immunofluorescence staining of Iba-1, CD86 and CD206. (D–F, J–L) Proportion of positive cells for target proteins. The number of samples in each group was 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.

Journal: Frontiers in Immunology

Article Title: Repetitive transcranial magnetic stimulation alleviates neuropathic pain via microglial polarization by modulating the METTL3/NMDAR2B/NLRP3 pathway

doi: 10.3389/fimmu.2025.1666920

Figure Lengend Snippet: The roles of METTL3 and NMDAR2B in the regulation of microglial cell polarization by magnetic stimulation in vitro . (A–C, G–I) Immunofluorescence staining of Iba-1, CD86 and CD206. (D–F, J–L) Proportion of positive cells for target proteins. The number of samples in each group was 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.

Article Snippet: After seeding, BV2 cells were cultured in complete DMEM for 24 h. Subsequently, specific pharmacological activation and inhibition targeting METTL3 (METTL3 activator-1; HY-W037893; MedChemExpress; STM2457; T9060; Targetmol) and NMDAR2B ( d -Serine; HY-100808; MedChemExpress; D-AP5; HY-100714A; MedChemExpress) were added to the culture medium, and their effects were verified.

Techniques: In Vitro, Immunofluorescence, Staining