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Advantages and disadvantages of DNA sequencing

Journal: Cancer Biology & Medicine

Article Title: The potential for liquid biopsies in the precision medical treatment of breast cancer

doi: 10.28092/j.issn.2095-3941.2016.0007

Figure Lengend Snippet: Advantages and disadvantages of DNA sequencing

Article Snippet: Pyrosequencing-sequencing by synthesis method , Chemiluminescent detection; DNA polymerase synthesizes cDNA to a target template; pyrophosphate release is detected at each base addition , More sensitive than Sanger; provides % of mutated vs . wild-type DNA; works well with fragmented DNA , Short read length limits technique to hot spots. Limited accuracy at detecting changes in homopolymer runs. Scalability is limited compared with other NGS methods , - .

Techniques: Sequencing, Electrophoresis, Multiplex Assay