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sema3a  (Shanghai Korain Biotech Co Ltd)
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pla  (Santa Cruz Biotechnology)
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sc 74555 rrid ab 2185394  (Santa Cruz Biotechnology)
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sema3a antibody c 1  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology sema3a antibody c 1
( A ) GSEA between the secretory (SEC) and window of implantation (WOI) groups for proliferative epithelium. ( B ) The single-cell pseudotime trajectory of proliferative epithelium, stromal cell, EMT-derived cell and stem-derived epithelium. Cells start at proliferative epithelium and progress to EMT-derived cell. There are seven major states over pseudotime. The black spot indicates the differentiation node between states 4, 5 and 6, indicating the direction from proliferative epithelium to EMT-derived cell. Arrows indicate the direction of the pseudotime trajectory. ( C ) The horizontal axis is the pseudotime point, and the vertical axis is the gene expression level. The solid line represents states 1, 2, 4, and 5 corresponding to Fig. S4B. Different colors represent samples in the CTRL, SEC, and WOI groups. ( D ) Heatmap of genes at the branch node regulating differentiation into EMT-derived cell and stem-derived epithelium. The horizontal axis is the pseudo-time point (the pseudo-time point gradually increases from the middle to both sides). The vertical axis is the gene expression level, representing two differential directions on the left and right sides. Clusters represent the gene sets with a similar branch gene expression trend. Different colors represent the level of gene expression. ( E–F ) Dot plots demonstrating the Cellphone DB analysis of relevant receptors and ligands of EMT-derived cell ( E ) or stromal cell ( F ) with other cell types. The size of the dot represents the level of significance. The color of the dot indicates the mean of the average expression level of interacting molecule 1 in EMT-derived cells ( E ) or stromal cells ( F ) and molecule 2 in other cell types. ( G ) Proximity ligation assay (PLA) validating the interactions of <t>SEMA3A-NRP1</t> and CD46-JAG1 in the CTRL, SEC and WOI assembloids. Red signals the interaction of two proteins. Nuclei were counterstained with DAPI. Scale bar = 20 μm.
Sema3a Antibody C 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sema3a/pmc13043174-36-2-8?v=Santa+Cruz+Biotechnology
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protein response upr semaphorin 4a sema4a semaphorin 3a semaphorin 3a sema3a forkhead box protein p3  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology protein response upr semaphorin 4a sema4a semaphorin 3a semaphorin 3a sema3a forkhead box protein p3
( A ) GSEA between the secretory (SEC) and window of implantation (WOI) groups for proliferative epithelium. ( B ) The single-cell pseudotime trajectory of proliferative epithelium, stromal cell, EMT-derived cell and stem-derived epithelium. Cells start at proliferative epithelium and progress to EMT-derived cell. There are seven major states over pseudotime. The black spot indicates the differentiation node between states 4, 5 and 6, indicating the direction from proliferative epithelium to EMT-derived cell. Arrows indicate the direction of the pseudotime trajectory. ( C ) The horizontal axis is the pseudotime point, and the vertical axis is the gene expression level. The solid line represents states 1, 2, 4, and 5 corresponding to Fig. S4B. Different colors represent samples in the CTRL, SEC, and WOI groups. ( D ) Heatmap of genes at the branch node regulating differentiation into EMT-derived cell and stem-derived epithelium. The horizontal axis is the pseudo-time point (the pseudo-time point gradually increases from the middle to both sides). The vertical axis is the gene expression level, representing two differential directions on the left and right sides. Clusters represent the gene sets with a similar branch gene expression trend. Different colors represent the level of gene expression. ( E–F ) Dot plots demonstrating the Cellphone DB analysis of relevant receptors and ligands of EMT-derived cell ( E ) or stromal cell ( F ) with other cell types. The size of the dot represents the level of significance. The color of the dot indicates the mean of the average expression level of interacting molecule 1 in EMT-derived cells ( E ) or stromal cells ( F ) and molecule 2 in other cell types. ( G ) Proximity ligation assay (PLA) validating the interactions of <t>SEMA3A-NRP1</t> and CD46-JAG1 in the CTRL, SEC and WOI assembloids. Red signals the interaction of two proteins. Nuclei were counterstained with DAPI. Scale bar = 20 μm.
Protein Response Upr Semaphorin 4a Sema4a Semaphorin 3a Semaphorin 3a Sema3a Forkhead Box Protein P3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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endothelial cells  (OriGene)
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( A ) GSEA between the secretory (SEC) and window of implantation (WOI) groups for proliferative epithelium. ( B ) The single-cell pseudotime trajectory of proliferative epithelium, stromal cell, EMT-derived cell and stem-derived epithelium. Cells start at proliferative epithelium and progress to EMT-derived cell. There are seven major states over pseudotime. The black spot indicates the differentiation node between states 4, 5 and 6, indicating the direction from proliferative epithelium to EMT-derived cell. Arrows indicate the direction of the pseudotime trajectory. ( C ) The horizontal axis is the pseudotime point, and the vertical axis is the gene expression level. The solid line represents states 1, 2, 4, and 5 corresponding to Fig. S4B. Different colors represent samples in the CTRL, SEC, and WOI groups. ( D ) Heatmap of genes at the branch node regulating differentiation into EMT-derived cell and stem-derived epithelium. The horizontal axis is the pseudo-time point (the pseudo-time point gradually increases from the middle to both sides). The vertical axis is the gene expression level, representing two differential directions on the left and right sides. Clusters represent the gene sets with a similar branch gene expression trend. Different colors represent the level of gene expression. ( E–F ) Dot plots demonstrating the Cellphone DB analysis of relevant receptors and ligands of EMT-derived cell ( E ) or stromal cell ( F ) with other cell types. The size of the dot represents the level of significance. The color of the dot indicates the mean of the average expression level of interacting molecule 1 in EMT-derived cells ( E ) or stromal cells ( F ) and molecule 2 in other cell types. ( G ) Proximity ligation assay (PLA) validating the interactions of <t>SEMA3A-NRP1</t> and CD46-JAG1 in the CTRL, SEC and WOI assembloids. Red signals the interaction of two proteins. Nuclei were counterstained with DAPI. Scale bar = 20 μm.
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( A ) GSEA between the secretory (SEC) and window of implantation (WOI) groups for proliferative epithelium. ( B ) The single-cell pseudotime trajectory of proliferative epithelium, stromal cell, EMT-derived cell and stem-derived epithelium. Cells start at proliferative epithelium and progress to EMT-derived cell. There are seven major states over pseudotime. The black spot indicates the differentiation node between states 4, 5 and 6, indicating the direction from proliferative epithelium to EMT-derived cell. Arrows indicate the direction of the pseudotime trajectory. ( C ) The horizontal axis is the pseudotime point, and the vertical axis is the gene expression level. The solid line represents states 1, 2, 4, and 5 corresponding to Fig. S4B. Different colors represent samples in the CTRL, SEC, and WOI groups. ( D ) Heatmap of genes at the branch node regulating differentiation into EMT-derived cell and stem-derived epithelium. The horizontal axis is the pseudo-time point (the pseudo-time point gradually increases from the middle to both sides). The vertical axis is the gene expression level, representing two differential directions on the left and right sides. Clusters represent the gene sets with a similar branch gene expression trend. Different colors represent the level of gene expression. ( E–F ) Dot plots demonstrating the Cellphone DB analysis of relevant receptors and ligands of EMT-derived cell ( E ) or stromal cell ( F ) with other cell types. The size of the dot represents the level of significance. The color of the dot indicates the mean of the average expression level of interacting molecule 1 in EMT-derived cells ( E ) or stromal cells ( F ) and molecule 2 in other cell types. ( G ) Proximity ligation assay (PLA) validating the interactions of <t>SEMA3A-NRP1</t> and CD46-JAG1 in the CTRL, SEC and WOI assembloids. Red signals the interaction of two proteins. Nuclei were counterstained with DAPI. Scale bar = 20 μm.
Sema3a, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human sema3a elisa kit  (Elabscience Biotechnology)
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( A ) GSEA between the secretory (SEC) and window of implantation (WOI) groups for proliferative epithelium. ( B ) The single-cell pseudotime trajectory of proliferative epithelium, stromal cell, EMT-derived cell and stem-derived epithelium. Cells start at proliferative epithelium and progress to EMT-derived cell. There are seven major states over pseudotime. The black spot indicates the differentiation node between states 4, 5 and 6, indicating the direction from proliferative epithelium to EMT-derived cell. Arrows indicate the direction of the pseudotime trajectory. ( C ) The horizontal axis is the pseudotime point, and the vertical axis is the gene expression level. The solid line represents states 1, 2, 4, and 5 corresponding to Fig. S4B. Different colors represent samples in the CTRL, SEC, and WOI groups. ( D ) Heatmap of genes at the branch node regulating differentiation into EMT-derived cell and stem-derived epithelium. The horizontal axis is the pseudo-time point (the pseudo-time point gradually increases from the middle to both sides). The vertical axis is the gene expression level, representing two differential directions on the left and right sides. Clusters represent the gene sets with a similar branch gene expression trend. Different colors represent the level of gene expression. ( E–F ) Dot plots demonstrating the Cellphone DB analysis of relevant receptors and ligands of EMT-derived cell ( E ) or stromal cell ( F ) with other cell types. The size of the dot represents the level of significance. The color of the dot indicates the mean of the average expression level of interacting molecule 1 in EMT-derived cells ( E ) or stromal cells ( F ) and molecule 2 in other cell types. ( G ) Proximity ligation assay (PLA) validating the interactions of <t>SEMA3A-NRP1</t> and CD46-JAG1 in the CTRL, SEC and WOI assembloids. Red signals the interaction of two proteins. Nuclei were counterstained with DAPI. Scale bar = 20 μm.
Human Sema3a Elisa Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sema3a protein concentration  (Elabscience Biotechnology)
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Elabscience Biotechnology sema3a protein concentration
( A ) GSEA between the secretory (SEC) and window of implantation (WOI) groups for proliferative epithelium. ( B ) The single-cell pseudotime trajectory of proliferative epithelium, stromal cell, EMT-derived cell and stem-derived epithelium. Cells start at proliferative epithelium and progress to EMT-derived cell. There are seven major states over pseudotime. The black spot indicates the differentiation node between states 4, 5 and 6, indicating the direction from proliferative epithelium to EMT-derived cell. Arrows indicate the direction of the pseudotime trajectory. ( C ) The horizontal axis is the pseudotime point, and the vertical axis is the gene expression level. The solid line represents states 1, 2, 4, and 5 corresponding to Fig. S4B. Different colors represent samples in the CTRL, SEC, and WOI groups. ( D ) Heatmap of genes at the branch node regulating differentiation into EMT-derived cell and stem-derived epithelium. The horizontal axis is the pseudo-time point (the pseudo-time point gradually increases from the middle to both sides). The vertical axis is the gene expression level, representing two differential directions on the left and right sides. Clusters represent the gene sets with a similar branch gene expression trend. Different colors represent the level of gene expression. ( E–F ) Dot plots demonstrating the Cellphone DB analysis of relevant receptors and ligands of EMT-derived cell ( E ) or stromal cell ( F ) with other cell types. The size of the dot represents the level of significance. The color of the dot indicates the mean of the average expression level of interacting molecule 1 in EMT-derived cells ( E ) or stromal cells ( F ) and molecule 2 in other cell types. ( G ) Proximity ligation assay (PLA) validating the interactions of <t>SEMA3A-NRP1</t> and CD46-JAG1 in the CTRL, SEC and WOI assembloids. Red signals the interaction of two proteins. Nuclei were counterstained with DAPI. Scale bar = 20 μm.
Sema3a Protein Concentration, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sema3a/10__1016_slash_j__jdermsci__2025__12__001-83-0-11?v=Elabscience+Biotechnology
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Image Search Results


( A ) GSEA between the secretory (SEC) and window of implantation (WOI) groups for proliferative epithelium. ( B ) The single-cell pseudotime trajectory of proliferative epithelium, stromal cell, EMT-derived cell and stem-derived epithelium. Cells start at proliferative epithelium and progress to EMT-derived cell. There are seven major states over pseudotime. The black spot indicates the differentiation node between states 4, 5 and 6, indicating the direction from proliferative epithelium to EMT-derived cell. Arrows indicate the direction of the pseudotime trajectory. ( C ) The horizontal axis is the pseudotime point, and the vertical axis is the gene expression level. The solid line represents states 1, 2, 4, and 5 corresponding to Fig. S4B. Different colors represent samples in the CTRL, SEC, and WOI groups. ( D ) Heatmap of genes at the branch node regulating differentiation into EMT-derived cell and stem-derived epithelium. The horizontal axis is the pseudo-time point (the pseudo-time point gradually increases from the middle to both sides). The vertical axis is the gene expression level, representing two differential directions on the left and right sides. Clusters represent the gene sets with a similar branch gene expression trend. Different colors represent the level of gene expression. ( E–F ) Dot plots demonstrating the Cellphone DB analysis of relevant receptors and ligands of EMT-derived cell ( E ) or stromal cell ( F ) with other cell types. The size of the dot represents the level of significance. The color of the dot indicates the mean of the average expression level of interacting molecule 1 in EMT-derived cells ( E ) or stromal cells ( F ) and molecule 2 in other cell types. ( G ) Proximity ligation assay (PLA) validating the interactions of SEMA3A-NRP1 and CD46-JAG1 in the CTRL, SEC and WOI assembloids. Red signals the interaction of two proteins. Nuclei were counterstained with DAPI. Scale bar = 20 μm.

Journal: eLife

Article Title: Human receptive endometrial assembloid for deciphering the implantation window

doi: 10.7554/eLife.90729

Figure Lengend Snippet: ( A ) GSEA between the secretory (SEC) and window of implantation (WOI) groups for proliferative epithelium. ( B ) The single-cell pseudotime trajectory of proliferative epithelium, stromal cell, EMT-derived cell and stem-derived epithelium. Cells start at proliferative epithelium and progress to EMT-derived cell. There are seven major states over pseudotime. The black spot indicates the differentiation node between states 4, 5 and 6, indicating the direction from proliferative epithelium to EMT-derived cell. Arrows indicate the direction of the pseudotime trajectory. ( C ) The horizontal axis is the pseudotime point, and the vertical axis is the gene expression level. The solid line represents states 1, 2, 4, and 5 corresponding to Fig. S4B. Different colors represent samples in the CTRL, SEC, and WOI groups. ( D ) Heatmap of genes at the branch node regulating differentiation into EMT-derived cell and stem-derived epithelium. The horizontal axis is the pseudo-time point (the pseudo-time point gradually increases from the middle to both sides). The vertical axis is the gene expression level, representing two differential directions on the left and right sides. Clusters represent the gene sets with a similar branch gene expression trend. Different colors represent the level of gene expression. ( E–F ) Dot plots demonstrating the Cellphone DB analysis of relevant receptors and ligands of EMT-derived cell ( E ) or stromal cell ( F ) with other cell types. The size of the dot represents the level of significance. The color of the dot indicates the mean of the average expression level of interacting molecule 1 in EMT-derived cells ( E ) or stromal cells ( F ) and molecule 2 in other cell types. ( G ) Proximity ligation assay (PLA) validating the interactions of SEMA3A-NRP1 and CD46-JAG1 in the CTRL, SEC and WOI assembloids. Red signals the interaction of two proteins. Nuclei were counterstained with DAPI. Scale bar = 20 μm.

Article Snippet: Antibody , SEMA3A antibody (C-1) (Mouse monoclonal) , Santa Cruz , Cat#: sc-74555 RRID: AB_2185394 , PLA (1:50).

Techniques: Single Cell, Derivative Assay, Gene Expression, Expressing, Proximity Ligation Assay