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Increased long-term specificity and efficiency for the minimal hIBA1a promoter when packaged in <t>scAAV</t> (A) Schematic diagram of the experimental procedure. scAAV5 virus with the hIBA1a promoter was injected into sham mice or mice with L-NIO-induced stroke. Brains were analyzed 1 week or 4 weeks later. (B) Representative confocal images showing marker expression for the indicated conditions. Scale bars, 50 μm. (C) Quantifications showing microglia-specificity of GFP expression for the indicated conditions (mean ± SEM; n = 3 mice per group). (D) Quantifications showing microglia transduction efficiency for the indicated conditions (mean ± SEM; n = 3 mice per group).
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Increased long-term specificity and efficiency for the minimal hIBA1a promoter when packaged in <t>scAAV</t> (A) Schematic diagram of the experimental procedure. scAAV5 virus with the hIBA1a promoter was injected into sham mice or mice with L-NIO-induced stroke. Brains were analyzed 1 week or 4 weeks later. (B) Representative confocal images showing marker expression for the indicated conditions. Scale bars, 50 μm. (C) Quantifications showing microglia-specificity of GFP expression for the indicated conditions (mean ± SEM; n = 3 mice per group). (D) Quantifications showing microglia transduction efficiency for the indicated conditions (mean ± SEM; n = 3 mice per group).
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Increased long-term specificity and efficiency for the minimal hIBA1a promoter when packaged in scAAV (A) Schematic diagram of the experimental procedure. scAAV5 virus with the hIBA1a promoter was injected into sham mice or mice with L-NIO-induced stroke. Brains were analyzed 1 week or 4 weeks later. (B) Representative confocal images showing marker expression for the indicated conditions. Scale bars, 50 μm. (C) Quantifications showing microglia-specificity of GFP expression for the indicated conditions (mean ± SEM; n = 3 mice per group). (D) Quantifications showing microglia transduction efficiency for the indicated conditions (mean ± SEM; n = 3 mice per group).

Journal: iScience

Article Title: Simple and highly specific targeting of resident microglia with adeno-associated virus

doi: 10.1016/j.isci.2024.110706

Figure Lengend Snippet: Increased long-term specificity and efficiency for the minimal hIBA1a promoter when packaged in scAAV (A) Schematic diagram of the experimental procedure. scAAV5 virus with the hIBA1a promoter was injected into sham mice or mice with L-NIO-induced stroke. Brains were analyzed 1 week or 4 weeks later. (B) Representative confocal images showing marker expression for the indicated conditions. Scale bars, 50 μm. (C) Quantifications showing microglia-specificity of GFP expression for the indicated conditions (mean ± SEM; n = 3 mice per group). (D) Quantifications showing microglia transduction efficiency for the indicated conditions (mean ± SEM; n = 3 mice per group).

Article Snippet: The self-complementary AAV (scAAV) vectors were based on the scAAV-CAG-GFP vector (Addgene #83279) by replacing the CAG promoter with the hIBA1 promoter.

Techniques: Virus, Injection, Marker, Expressing, Transduction

Journal: iScience

Article Title: Simple and highly specific targeting of resident microglia with adeno-associated virus

doi: 10.1016/j.isci.2024.110706

Figure Lengend Snippet:

Article Snippet: The self-complementary AAV (scAAV) vectors were based on the scAAV-CAG-GFP vector (Addgene #83279) by replacing the CAG promoter with the hIBA1 promoter.

Techniques: Recombinant, Software