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Journal: Translational Oncology
Article Title: Overcoming the leptomeningeal seeding of medulloblastoma by targeting HSP70
doi: 10.1016/j.tranon.2026.102695
Figure Lengend Snippet: Phenotypic differences between LMS-derived seeding (S3) and non-seeding (N3) cells. (A) Morphological differences were not observed between S3 and N3 under bright field microscope. (B) The graph shows that the proliferative capacity of S3 is slower than that of N3s through High-Content Screening (HCS) analysis. (C) The trans-well migration assay reveals that there was no significant difference in the migration ability between S3 and N3 (quantification graph). (D) Representative images from the trans-well migration assay. (E) Representative images of the wound-healing assay show that N3 filled the open area faster than S3. The white area is saturated with green color due to the overlapping of S3 without filling the wound gap. (F) Quantification graph of the wound-healing assay. (G) Adhesion of S3 and N3 cells to extracellular matrix (ECM) components, including fibronectin, collagen I, collagen IV, laminin I, and fibrinogen, was quantified after 24h of incubation. (H) Adhesion values were normalized to control conditions. All data are presented as mean ± SD from n = 3 independent experiments. Statistical comparisons between two groups were performed using a two-tailed Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001.
Article Snippet: Cell proliferation was monitored and quantified consecutively for 64 hours using a
Techniques: Derivative Assay, Microscopy, High Content Screening, Migration, Wound Healing Assay, Incubation, Control, Two Tailed Test