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S-adenosylmethionine was increased 3 days post-rmTBI in the hippocampus after repetitive and mild traumatic brain injury (rmTBI). ELISA was performed to measure S-adenosylmethionine <t>(SAM)</t> <t>levels</t> in the cortex and hippocampus of mice tissue lysates. (A) There were no significant differences in SAM concentration in the cortex, however, the levels followed the same trend seen in the hippocampus. (B) There was a significant elevation of SAM levels within the hippocampus 3 days (blue bar) post-rmTBI relative to SHAM (black bar), followed by a significant decrease at 7 days (blue bar) relative to 3 days post-rmTBI. Results were expressed as mean ± SEM. *p ≤ 0.05, **p ≤ 0.01, evaluated by one-way ANOVA with Tukey’s post-hoc test, (n = 4–5).
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Intracel Corp intracel- lular sam-level changes
S-adenosylmethionine was increased 3 days post-rmTBI in the hippocampus after repetitive and mild traumatic brain injury (rmTBI). ELISA was performed to measure S-adenosylmethionine <t>(SAM)</t> <t>levels</t> in the cortex and hippocampus of mice tissue lysates. (A) There were no significant differences in SAM concentration in the cortex, however, the levels followed the same trend seen in the hippocampus. (B) There was a significant elevation of SAM levels within the hippocampus 3 days (blue bar) post-rmTBI relative to SHAM (black bar), followed by a significant decrease at 7 days (blue bar) relative to 3 days post-rmTBI. Results were expressed as mean ± SEM. *p ≤ 0.05, **p ≤ 0.01, evaluated by one-way ANOVA with Tukey’s post-hoc test, (n = 4–5).
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S-adenosylmethionine was increased 3 days post-rmTBI in the hippocampus after repetitive and mild traumatic brain injury (rmTBI). ELISA was performed to measure S-adenosylmethionine (SAM) levels in the cortex and hippocampus of mice tissue lysates. (A) There were no significant differences in SAM concentration in the cortex, however, the levels followed the same trend seen in the hippocampus. (B) There was a significant elevation of SAM levels within the hippocampus 3 days (blue bar) post-rmTBI relative to SHAM (black bar), followed by a significant decrease at 7 days (blue bar) relative to 3 days post-rmTBI. Results were expressed as mean ± SEM. *p ≤ 0.05, **p ≤ 0.01, evaluated by one-way ANOVA with Tukey’s post-hoc test, (n = 4–5).

Journal: Neurochemistry international

Article Title: A role for protein arginine methyltransferase 7 in repetitive and mild traumatic brain injury

doi: 10.1016/j.neuint.2023.105524

Figure Lengend Snippet: S-adenosylmethionine was increased 3 days post-rmTBI in the hippocampus after repetitive and mild traumatic brain injury (rmTBI). ELISA was performed to measure S-adenosylmethionine (SAM) levels in the cortex and hippocampus of mice tissue lysates. (A) There were no significant differences in SAM concentration in the cortex, however, the levels followed the same trend seen in the hippocampus. (B) There was a significant elevation of SAM levels within the hippocampus 3 days (blue bar) post-rmTBI relative to SHAM (black bar), followed by a significant decrease at 7 days (blue bar) relative to 3 days post-rmTBI. Results were expressed as mean ± SEM. *p ≤ 0.05, **p ≤ 0.01, evaluated by one-way ANOVA with Tukey’s post-hoc test, (n = 4–5).

Article Snippet: SAM levels were detected in tissue lysates of both the cerebral cortex and hippocampus via FLUOstar Omega (BMG Labtech; S/N: 415–2374) and adhered to protocols provided by the manufacturer.

Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay