Review





Similar Products

rt-pcr-pyrosequencing assay  (Pyrosequencing Inc)
90
Pyrosequencing Inc rt-pcr-pyrosequencing assay
Schematic representation of the location of forward and reverse PCR primers (arrows) used to amplify four regions of the S gene of SARS‐CoV‐2. PCR 1 and PCR 2 target sequences corresponding to the N‐terminal domain (NTD) of the <t>S</t> <t>protein</t> and overlap by 11 bases. PCR 2 resides within the receptor‐binding domain (RBD), and PCR 4 overlies the junction between the S1 and S2 subunits (S1/S2). Common S gene mutations reported in SARS‐CoV‐2 variants, detectable by pyrosequencing the PCR products, using sequencing primers or forward PCR primers as listed in Table , are indicated in the respective boxes. Forty‐two mutations detected in specimens analyzed in the current study appear in bold type. Other rare, novel mutations may be detected within targeted sequences but are not listed.
Rt Pcr Pyrosequencing Assay, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rt-pcr-pyrosequencing assay/product/Pyrosequencing Inc
Average 90 stars, based on 1 article reviews
rt-pcr-pyrosequencing assay - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
rt-pcr-pyrosequencing  (Pyrosequencing Inc)
90
Pyrosequencing Inc rt-pcr-pyrosequencing
Schematic representation of the location of forward and reverse PCR primers (arrows) used to amplify four regions of the S gene of SARS‐CoV‐2. PCR 1 and PCR 2 target sequences corresponding to the N‐terminal domain (NTD) of the <t>S</t> <t>protein</t> and overlap by 11 bases. PCR 2 resides within the receptor‐binding domain (RBD), and PCR 4 overlies the junction between the S1 and S2 subunits (S1/S2). Common S gene mutations reported in SARS‐CoV‐2 variants, detectable by pyrosequencing the PCR products, using sequencing primers or forward PCR primers as listed in Table , are indicated in the respective boxes. Forty‐two mutations detected in specimens analyzed in the current study appear in bold type. Other rare, novel mutations may be detected within targeted sequences but are not listed.
Rt Pcr Pyrosequencing, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rt-pcr-pyrosequencing/product/Pyrosequencing Inc
Average 90 stars, based on 1 article reviews
rt-pcr-pyrosequencing - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
pyrosequencing/rt-pcr  (Pyrosequencing Inc)
90
Pyrosequencing Inc pyrosequencing/rt-pcr
Schematic representation of the location of forward and reverse PCR primers (arrows) used to amplify four regions of the S gene of SARS‐CoV‐2. PCR 1 and PCR 2 target sequences corresponding to the N‐terminal domain (NTD) of the <t>S</t> <t>protein</t> and overlap by 11 bases. PCR 2 resides within the receptor‐binding domain (RBD), and PCR 4 overlies the junction between the S1 and S2 subunits (S1/S2). Common S gene mutations reported in SARS‐CoV‐2 variants, detectable by pyrosequencing the PCR products, using sequencing primers or forward PCR primers as listed in Table , are indicated in the respective boxes. Forty‐two mutations detected in specimens analyzed in the current study appear in bold type. Other rare, novel mutations may be detected within targeted sequences but are not listed.
Pyrosequencing/Rt Pcr, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pyrosequencing/rt-pcr/product/Pyrosequencing Inc
Average 90 stars, based on 1 article reviews
pyrosequencing/rt-pcr - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
rt-pcr pyrosequencing  (Pyrosequencing Inc)
90
Pyrosequencing Inc rt-pcr pyrosequencing
Schematic representation of the location of forward and reverse PCR primers (arrows) used to amplify four regions of the S gene of SARS‐CoV‐2. PCR 1 and PCR 2 target sequences corresponding to the N‐terminal domain (NTD) of the <t>S</t> <t>protein</t> and overlap by 11 bases. PCR 2 resides within the receptor‐binding domain (RBD), and PCR 4 overlies the junction between the S1 and S2 subunits (S1/S2). Common S gene mutations reported in SARS‐CoV‐2 variants, detectable by pyrosequencing the PCR products, using sequencing primers or forward PCR primers as listed in Table , are indicated in the respective boxes. Forty‐two mutations detected in specimens analyzed in the current study appear in bold type. Other rare, novel mutations may be detected within targeted sequences but are not listed.
Rt Pcr Pyrosequencing, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rt-pcr pyrosequencing/product/Pyrosequencing Inc
Average 90 stars, based on 1 article reviews
rt-pcr pyrosequencing - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
combined reverse-transcription polymerase chain reaction (rt-pcr)/pyrosequencing approach  (Pyrosequencing Inc)
90
Pyrosequencing Inc combined reverse-transcription polymerase chain reaction (rt-pcr)/pyrosequencing approach
Schematic representation of the location of forward and reverse PCR primers (arrows) used to amplify four regions of the S gene of SARS‐CoV‐2. PCR 1 and PCR 2 target sequences corresponding to the N‐terminal domain (NTD) of the <t>S</t> <t>protein</t> and overlap by 11 bases. PCR 2 resides within the receptor‐binding domain (RBD), and PCR 4 overlies the junction between the S1 and S2 subunits (S1/S2). Common S gene mutations reported in SARS‐CoV‐2 variants, detectable by pyrosequencing the PCR products, using sequencing primers or forward PCR primers as listed in Table , are indicated in the respective boxes. Forty‐two mutations detected in specimens analyzed in the current study appear in bold type. Other rare, novel mutations may be detected within targeted sequences but are not listed.
Combined Reverse Transcription Polymerase Chain Reaction (Rt Pcr)/Pyrosequencing Approach, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/combined reverse-transcription polymerase chain reaction (rt-pcr)/pyrosequencing approach/product/Pyrosequencing Inc
Average 90 stars, based on 1 article reviews
combined reverse-transcription polymerase chain reaction (rt-pcr)/pyrosequencing approach - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
pyrosequencing rt-pcr  (Pyrosequencing Inc)
90
Pyrosequencing Inc pyrosequencing rt-pcr
Schematic representation of the location of forward and reverse PCR primers (arrows) used to amplify four regions of the S gene of SARS‐CoV‐2. PCR 1 and PCR 2 target sequences corresponding to the N‐terminal domain (NTD) of the <t>S</t> <t>protein</t> and overlap by 11 bases. PCR 2 resides within the receptor‐binding domain (RBD), and PCR 4 overlies the junction between the S1 and S2 subunits (S1/S2). Common S gene mutations reported in SARS‐CoV‐2 variants, detectable by pyrosequencing the PCR products, using sequencing primers or forward PCR primers as listed in Table , are indicated in the respective boxes. Forty‐two mutations detected in specimens analyzed in the current study appear in bold type. Other rare, novel mutations may be detected within targeted sequences but are not listed.
Pyrosequencing Rt Pcr, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pyrosequencing rt-pcr/product/Pyrosequencing Inc
Average 90 stars, based on 1 article reviews
pyrosequencing rt-pcr - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
primers for rt-pcr and pyrosequencing  (BIOTAGE)
90
BIOTAGE primers for rt-pcr and pyrosequencing
Schematic representation of the location of forward and reverse PCR primers (arrows) used to amplify four regions of the S gene of SARS‐CoV‐2. PCR 1 and PCR 2 target sequences corresponding to the N‐terminal domain (NTD) of the <t>S</t> <t>protein</t> and overlap by 11 bases. PCR 2 resides within the receptor‐binding domain (RBD), and PCR 4 overlies the junction between the S1 and S2 subunits (S1/S2). Common S gene mutations reported in SARS‐CoV‐2 variants, detectable by pyrosequencing the PCR products, using sequencing primers or forward PCR primers as listed in Table , are indicated in the respective boxes. Forty‐two mutations detected in specimens analyzed in the current study appear in bold type. Other rare, novel mutations may be detected within targeted sequences but are not listed.
Primers For Rt Pcr And Pyrosequencing, supplied by BIOTAGE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primers for rt-pcr and pyrosequencing/product/BIOTAGE
Average 90 stars, based on 1 article reviews
primers for rt-pcr and pyrosequencing - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
rt-pcr pyrosequencing assay  (Pyrosequencing Inc)
90
Pyrosequencing Inc rt-pcr pyrosequencing assay
Schematic representation of the location of forward and reverse PCR primers (arrows) used to amplify four regions of the S gene of SARS‐CoV‐2. PCR 1 and PCR 2 target sequences corresponding to the N‐terminal domain (NTD) of the <t>S</t> <t>protein</t> and overlap by 11 bases. PCR 2 resides within the receptor‐binding domain (RBD), and PCR 4 overlies the junction between the S1 and S2 subunits (S1/S2). Common S gene mutations reported in SARS‐CoV‐2 variants, detectable by pyrosequencing the PCR products, using sequencing primers or forward PCR primers as listed in Table , are indicated in the respective boxes. Forty‐two mutations detected in specimens analyzed in the current study appear in bold type. Other rare, novel mutations may be detected within targeted sequences but are not listed.
Rt Pcr Pyrosequencing Assay, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rt-pcr pyrosequencing assay/product/Pyrosequencing Inc
Average 90 stars, based on 1 article reviews
rt-pcr pyrosequencing assay - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier

Image Search Results


Schematic representation of the location of forward and reverse PCR primers (arrows) used to amplify four regions of the S gene of SARS‐CoV‐2. PCR 1 and PCR 2 target sequences corresponding to the N‐terminal domain (NTD) of the S protein and overlap by 11 bases. PCR 2 resides within the receptor‐binding domain (RBD), and PCR 4 overlies the junction between the S1 and S2 subunits (S1/S2). Common S gene mutations reported in SARS‐CoV‐2 variants, detectable by pyrosequencing the PCR products, using sequencing primers or forward PCR primers as listed in Table , are indicated in the respective boxes. Forty‐two mutations detected in specimens analyzed in the current study appear in bold type. Other rare, novel mutations may be detected within targeted sequences but are not listed.

Journal: Journal of Medical Virology

Article Title: A pyrosequencing protocol for rapid identification of SARS‐CoV‐2 variants

doi: 10.1002/jmv.27770

Figure Lengend Snippet: Schematic representation of the location of forward and reverse PCR primers (arrows) used to amplify four regions of the S gene of SARS‐CoV‐2. PCR 1 and PCR 2 target sequences corresponding to the N‐terminal domain (NTD) of the S protein and overlap by 11 bases. PCR 2 resides within the receptor‐binding domain (RBD), and PCR 4 overlies the junction between the S1 and S2 subunits (S1/S2). Common S gene mutations reported in SARS‐CoV‐2 variants, detectable by pyrosequencing the PCR products, using sequencing primers or forward PCR primers as listed in Table , are indicated in the respective boxes. Forty‐two mutations detected in specimens analyzed in the current study appear in bold type. Other rare, novel mutations may be detected within targeted sequences but are not listed.

Article Snippet: The current study describes the development of a reverse transcription (RT)‐PCR‐pyrosequencing assay targeting >65 spike protein gene ( S ) mutations of SARS‐CoV‐2, which permits differentiation of commonly reported variants currently circulating in the United States with a high degree of confidence.

Techniques: Binding Assay, Sequencing