Journal: Clinical Epigenetics

Article Title: An epigenomic investigation of atrial fibrillation in a matched left and right atrial human cohort

doi: 10.1186/s13148-026-02076-2

Figure Lengend Snippet: Targeted validation of gene expression levels at candidate loci in an independent replication cohort. Locus-specific comparison of gene expression levels in AF and SR samples for selected candidate loci in the discovery cohort (top boxplots in each category, RNA-seq), and their validation in an independent replication set (bottom barplots in each category, RT-qPCR). RT-qPCR results are represented as relative expression levels over those of TBP as a reference gene. Gene expression differences across disease status were considered as validated in independent samples when the direction of change was maintained with a p-value < 0.2 (one-sided t-test). a Gene expression levels in the discovery cohort (top boxplots) and validation cohort (bottom barplots) across 8 AF-enriched candidate loci. b As in a. for five SR-enriched candidate loci. See also Figure S5 and Table S7

Article Snippet: 1000 ng of RNA were reversed transcribed into complementary DNA (cDNA) using the High-Capacity cDNA Reverse Transcription Kit (Fisher Scientific), and RT-qPCR reactions were performed using 5 ng of template cDNA.

Techniques: Biomarker Discovery, Gene Expression, Comparison, RNA Sequencing, Quantitative RT-PCR, Expressing