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Exosomes were collected from the spent medium of the medulloblastoma cell line D283MED by filtration and differential centrifugation. Vesicles were analyzed by dynamic light scattering (DLS) ( A, left ) and nanoparticle tracking with a NanoSight device ( A, right ) for vesicle diameters and concentration. Sizes at identified peaks are listed. Accounting for dilution, exosome concentration in this case was 2.84×10 8 particles/ml. In ( B ), D283MED exosomes were subjected to density-gradient centrifugation thru a 0%–60% Opti-Prep step gradient; fractions were collected and densities determined. Fractions containing exosomes were identified by acetylcholinesterase (AChE) activity and electron microscopy (micrographs in inset above peak fractions 8 and 9; bar = 100 nm). Exosomes were also fractionated by <t>Rotofor</t> free-solution isoelectric focusing ( C ) as described in the . Fractions were harvested and the pH of each was determined. Exosome-containing fractions were again identified by AChE activity.
Rotofor Device, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rotofor+device/pmc03407172-188-9-11?v=Bio-Rad
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rotofor device - by Bioz Stars, 2026-07
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micro rotofor device  (Bio-Rad)
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Exosomes were collected from the spent medium of the medulloblastoma cell line D283MED by filtration and differential centrifugation. Vesicles were analyzed by dynamic light scattering (DLS) ( A, left ) and nanoparticle tracking with a NanoSight device ( A, right ) for vesicle diameters and concentration. Sizes at identified peaks are listed. Accounting for dilution, exosome concentration in this case was 2.84×10 8 particles/ml. In ( B ), D283MED exosomes were subjected to density-gradient centrifugation thru a 0%–60% Opti-Prep step gradient; fractions were collected and densities determined. Fractions containing exosomes were identified by acetylcholinesterase (AChE) activity and electron microscopy (micrographs in inset above peak fractions 8 and 9; bar = 100 nm). Exosomes were also fractionated by <t>Rotofor</t> free-solution isoelectric focusing ( C ) as described in the . Fractions were harvested and the pH of each was determined. Exosome-containing fractions were again identified by AChE activity.
Micro Rotofor Device, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rotofor+device/pm28842203-51-27-29?v=Bio-Rad
Average 93 stars, based on 1 article reviews
micro rotofor device - by Bioz Stars, 2026-07
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devices for preparative isoelectric focusing rotofor  (Isoprime Ltd)
90
Isoprime Ltd devices for preparative isoelectric focusing rotofor
Exosomes were collected from the spent medium of the medulloblastoma cell line D283MED by filtration and differential centrifugation. Vesicles were analyzed by dynamic light scattering (DLS) ( A, left ) and nanoparticle tracking with a NanoSight device ( A, right ) for vesicle diameters and concentration. Sizes at identified peaks are listed. Accounting for dilution, exosome concentration in this case was 2.84×10 8 particles/ml. In ( B ), D283MED exosomes were subjected to density-gradient centrifugation thru a 0%–60% Opti-Prep step gradient; fractions were collected and densities determined. Fractions containing exosomes were identified by acetylcholinesterase (AChE) activity and electron microscopy (micrographs in inset above peak fractions 8 and 9; bar = 100 nm). Exosomes were also fractionated by <t>Rotofor</t> free-solution isoelectric focusing ( C ) as described in the . Fractions were harvested and the pH of each was determined. Exosome-containing fractions were again identified by AChE activity.
Devices For Preparative Isoelectric Focusing Rotofor, supplied by Isoprime Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rotofor+device/pm19585533-18-9-10?v=Isoprime+Ltd
Average 90 stars, based on 1 article reviews
devices for preparative isoelectric focusing rotofor - by Bioz Stars, 2026-07
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Exosomes were collected from the spent medium of the medulloblastoma cell line D283MED by filtration and differential centrifugation. Vesicles were analyzed by dynamic light scattering (DLS) ( A, left ) and nanoparticle tracking with a NanoSight device ( A, right ) for vesicle diameters and concentration. Sizes at identified peaks are listed. Accounting for dilution, exosome concentration in this case was 2.84×10 8 particles/ml. In ( B ), D283MED exosomes were subjected to density-gradient centrifugation thru a 0%–60% Opti-Prep step gradient; fractions were collected and densities determined. Fractions containing exosomes were identified by acetylcholinesterase (AChE) activity and electron microscopy (micrographs in inset above peak fractions 8 and 9; bar = 100 nm). Exosomes were also fractionated by Rotofor free-solution isoelectric focusing ( C ) as described in the . Fractions were harvested and the pH of each was determined. Exosome-containing fractions were again identified by AChE activity.

Journal: PLoS ONE

Article Title: Medulloblastoma Exosome Proteomics Yield Functional Roles for Extracellular Vesicles

doi: 10.1371/journal.pone.0042064

Figure Lengend Snippet: Exosomes were collected from the spent medium of the medulloblastoma cell line D283MED by filtration and differential centrifugation. Vesicles were analyzed by dynamic light scattering (DLS) ( A, left ) and nanoparticle tracking with a NanoSight device ( A, right ) for vesicle diameters and concentration. Sizes at identified peaks are listed. Accounting for dilution, exosome concentration in this case was 2.84×10 8 particles/ml. In ( B ), D283MED exosomes were subjected to density-gradient centrifugation thru a 0%–60% Opti-Prep step gradient; fractions were collected and densities determined. Fractions containing exosomes were identified by acetylcholinesterase (AChE) activity and electron microscopy (micrographs in inset above peak fractions 8 and 9; bar = 100 nm). Exosomes were also fractionated by Rotofor free-solution isoelectric focusing ( C ) as described in the . Fractions were harvested and the pH of each was determined. Exosome-containing fractions were again identified by AChE activity.

Article Snippet: Free-solution isoelectric focusing was performed as described using a Rotofor device (Bio-Rad Laboratories, Hercules, CA, USA).

Techniques: Filtration, Centrifugation, Concentration Assay, Gradient Centrifugation, Activity Assay, Electron Microscopy