Journal: eLife

Article Title: Ly6G + granulocytes-derived IL-17 limits protective host responses and promotes tuberculosis pathogenesis

doi: 10.7554/eLife.100966

Figure Lengend Snippet: ( A ) IL-17 levels in the lung homogenates of uninfected and Mtb -infected WT mice. An unpaired student’s t-test was applied to calculate significance between the two groups. Sample size (n)=5–6 mice/group ( B ) Study plan. C57BL/6 mice were infected with H37Rv at a 100–200 CFU dose via the aerosol route. At 3 weeks post-infection, treatment with celecoxib and SR 2211 (inverse agonist for RORγt) was started for a further 3 weeks. Celecoxib (50 mg/kg) was administered orally once a day, and SR 2211 (20mg/kg) was administered intraperitoneally thrice in a week. At 6 weeks post-infection, mice from the treatment and untreated control groups were sacrificed, lung and spleen were harvested for CFU, histopathology, and FACS analysis, and lung homogenates were stored for ELISA. ( C ) PGE2 levels in the lung homogenates of uninfected and Mtb -infected WT mice at 4 and 12 weeks post-infection time point. Unpaired student’s t-test was applied to calculate significance between the two groups at each time point. Sample size (n)=3–4 mice/group. ( D ) Levels of IL-17 in the lung homogenates of all four-treatment groups of mice. The statistical significance of the treatment groups was assessed by applying a one-way ANOVA test, n=3–4 mice/group. ( E ) Representative FACS dot plot showing the percentage of PMNs in the lungs of Mtb -infected mice in all four above-stated treatment groups (population is gated on CD45 + cells). Mycobacterial burden in the lung ( F ) and spleen ( G ), in the above-stated treatment groups of mice. ( H ) Lung PMNs Mtb burden normalized to per 10 4 sorted cells in all four treatment groups of mice. The statistical significance of the treatment groups was assessed by applying a one-way ANOVA test, n=5 mice/group. ( I ) Lung IFA images from the above-stated treated group of mice show the colocalization of PMNs (Ly6G + cells shown in red color) and IL-17 (shown in green color). The IL-17 + Ly6G + (double-positive) cells were quantified and represented as a percentage of DAPI + cells in all four treatment groups. This experiment was performed only once as a single experimental replicate.

Article Snippet: SR 2211, Selective RORγt inverse agonist (4869) was procured from TOCRIS.

Techniques: Infection, Aerosol, Control, Histopathology, Enzyme-linked Immunosorbent Assay

Journal: eLife

Article Title: Ly6G + granulocytes-derived IL-17 limits protective host responses and promotes tuberculosis pathogenesis

doi: 10.7554/eLife.100966

Figure Lengend Snippet: The experimental plan is shown in . C57BL/6 mice were infected with H37Rv at a 100–200 CFU dose via the aerosol route. At 3 weeks post-infection, treatment with celecoxib and SR 2211 (inverse agonist for RORγt) was started for a further 3 weeks. Celecoxib was administered orally once a day, and SR 2211 was administered intraperitoneally thrice a week. At 6 weeks post-infection, mice from the treatment and untreated control groups were sacrificed, and lung and spleen were harvested for CFU, histopathology, and FACS analysis. ( A ) Lung PMN count normalized to 1 million of total acquired cells in the above-stated treatment groups of mice. The statistical significance of the treatment groups was assessed by applying a one-way ANOVA test, n=5 mice/group. ( B ) H&E-stained lung sections showing pathology in SR2211+Celecoxib treatment group. ( C ) Granuloma scoring depicting lung pathology in four treatment groups of mice. n=5 mice per treatment group.

Article Snippet: SR 2211, Selective RORγt inverse agonist (4869) was procured from TOCRIS.

Techniques: Infection, Aerosol, Control, Histopathology, Staining

Journal: Frontiers in Nutrition

Article Title: Unveiling the pathways of Xuanbai Chengqi Decoction in obese asthma: from immune modulation to microbial restoration

doi: 10.3389/fnut.2026.1710739

Figure Lengend Snippet: XBCQD regulates Th17/Treg-related cytokines. (A,B) The protein expression of Foxp3 and RORγt in lung tissue after XBCQD treatment ( n = 5). (C) The mRNA expression of Foxp3 and RORγt in lung tissue after XBCQD treatment ( n = 5). (D) The expression of IL-10 and IL-17A in BALF after XBCQD treatment ( n = 5). (* p < 0.05, ** p < 0.01; as compared to the n group. # p < 0.05, ## p < 0.01; as compared to the fa group).

Article Snippet: The nonspecific binding sites in the transfer membrane were blocked with 5% skim milk at room temperature for 2 h. Imprinted membranes were incubated with primary antibodies: RORγt protein antibody (1:2000, CST, 4967), Foxp3 protein antibody (1:1000, SANTA, sc28343), GPR43 (1:1000, Proteintech, 19,952-1-AP), NLRP3 (1:1000, Cell Signaling Technology, 15101S), Caspase-1 (1:1000, ABclonal, A0964), IL-1β (1:2000, Bioss, bs-0812R).

Techniques: Expressing