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Ron Specific Sirna Si Ron#1, supplied by Shanghai Genechem Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 4: <t>RON</t> kinase is an important target of crizotinib in MPN (A) Western analysis of RON in SET2 cells treated with crizotinib and stimulated with erythropoietin (EPO), stem cell factor (SCF), and hepatocyte growth factor (HGF). Representative data from one of at least three independent experiments is shown (B) qPCR of MST1R (RON) expression in HEL cells transduced with 4 different MST1R <t>shRNA</t> constructs (C) Colony formation assay of transduced HEL cells in methylcellulose with myeloid growth factors. Experiments were performed at least three times, with similar results. ANOVA with Tukey’s multiple-comparison test was used. (D) Western analysis of RON, STAT3 and STAT5 phosphorylation in shRNA-transduced HEL cells stimulated with EPO, SCF, and HGF. Representative data from one of three independent experiments are shown. (E) Western analysis of JAK2 in shRNA-transduced HEL cells stimulated with EPO, SCF, and HGF (F) Flow cytometry analysis of RON phosphorylation in MPN patient or control donor peripheral blood CD45lo population with ex vivo EPO and SCF stimulation (G) Schematic for JAK2-V617F-GFP retroviral bone marrow transplantation using bone marrow from Stk−/− (RON−/−) mice (H) Peripheral blood analysis of WT (N=8) or Stk−/− (N=9) JAK2-V617F mice over time. WBC= white blood cells; Hb=hemoglobin; PLT=platelets (I) Percentage of GFP in the peripheral blood over time in WT (N=8) or
Human Shrna Plasmid Kits For Ron, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 4: <t>RON</t> kinase is an important target of crizotinib in MPN (A) Western analysis of RON in SET2 cells treated with crizotinib and stimulated with erythropoietin (EPO), stem cell factor (SCF), and hepatocyte growth factor (HGF). Representative data from one of at least three independent experiments is shown (B) qPCR of MST1R (RON) expression in HEL cells transduced with 4 different MST1R <t>shRNA</t> constructs (C) Colony formation assay of transduced HEL cells in methylcellulose with myeloid growth factors. Experiments were performed at least three times, with similar results. ANOVA with Tukey’s multiple-comparison test was used. (D) Western analysis of RON, STAT3 and STAT5 phosphorylation in shRNA-transduced HEL cells stimulated with EPO, SCF, and HGF. Representative data from one of three independent experiments are shown. (E) Western analysis of JAK2 in shRNA-transduced HEL cells stimulated with EPO, SCF, and HGF (F) Flow cytometry analysis of RON phosphorylation in MPN patient or control donor peripheral blood CD45lo population with ex vivo EPO and SCF stimulation (G) Schematic for JAK2-V617F-GFP retroviral bone marrow transplantation using bone marrow from Stk−/− (RON−/−) mice (H) Peripheral blood analysis of WT (N=8) or Stk−/− (N=9) JAK2-V617F mice over time. WBC= white blood cells; Hb=hemoglobin; PLT=platelets (I) Percentage of GFP in the peripheral blood over time in WT (N=8) or
Sirnas Targeted To The Ron Gene, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 4: <t>RON</t> kinase is an important target of crizotinib in MPN (A) Western analysis of RON in SET2 cells treated with crizotinib and stimulated with erythropoietin (EPO), stem cell factor (SCF), and hepatocyte growth factor (HGF). Representative data from one of at least three independent experiments is shown (B) qPCR of MST1R (RON) expression in HEL cells transduced with 4 different MST1R <t>shRNA</t> constructs (C) Colony formation assay of transduced HEL cells in methylcellulose with myeloid growth factors. Experiments were performed at least three times, with similar results. ANOVA with Tukey’s multiple-comparison test was used. (D) Western analysis of RON, STAT3 and STAT5 phosphorylation in shRNA-transduced HEL cells stimulated with EPO, SCF, and HGF. Representative data from one of three independent experiments are shown. (E) Western analysis of JAK2 in shRNA-transduced HEL cells stimulated with EPO, SCF, and HGF (F) Flow cytometry analysis of RON phosphorylation in MPN patient or control donor peripheral blood CD45lo population with ex vivo EPO and SCF stimulation (G) Schematic for JAK2-V617F-GFP retroviral bone marrow transplantation using bone marrow from Stk−/− (RON−/−) mice (H) Peripheral blood analysis of WT (N=8) or Stk−/− (N=9) JAK2-V617F mice over time. WBC= white blood cells; Hb=hemoglobin; PLT=platelets (I) Percentage of GFP in the peripheral blood over time in WT (N=8) or
Plko 1 Mkl1 2 Shrna Ron Prywes, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bioneer Corporation accutarget genome-wide predesigned ron-specific sirna #19882-1
Figure 4: <t>RON</t> kinase is an important target of crizotinib in MPN (A) Western analysis of RON in SET2 cells treated with crizotinib and stimulated with erythropoietin (EPO), stem cell factor (SCF), and hepatocyte growth factor (HGF). Representative data from one of at least three independent experiments is shown (B) qPCR of MST1R (RON) expression in HEL cells transduced with 4 different MST1R <t>shRNA</t> constructs (C) Colony formation assay of transduced HEL cells in methylcellulose with myeloid growth factors. Experiments were performed at least three times, with similar results. ANOVA with Tukey’s multiple-comparison test was used. (D) Western analysis of RON, STAT3 and STAT5 phosphorylation in shRNA-transduced HEL cells stimulated with EPO, SCF, and HGF. Representative data from one of three independent experiments are shown. (E) Western analysis of JAK2 in shRNA-transduced HEL cells stimulated with EPO, SCF, and HGF (F) Flow cytometry analysis of RON phosphorylation in MPN patient or control donor peripheral blood CD45lo population with ex vivo EPO and SCF stimulation (G) Schematic for JAK2-V617F-GFP retroviral bone marrow transplantation using bone marrow from Stk−/− (RON−/−) mice (H) Peripheral blood analysis of WT (N=8) or Stk−/− (N=9) JAK2-V617F mice over time. WBC= white blood cells; Hb=hemoglobin; PLT=platelets (I) Percentage of GFP in the peripheral blood over time in WT (N=8) or
Accutarget Genome Wide Predesigned Ron Specific Sirna #19882 1, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Santa Cruz Biotechnology ron
Figure 4: <t>RON</t> kinase is an important target of crizotinib in MPN (A) Western analysis of RON in SET2 cells treated with crizotinib and stimulated with erythropoietin (EPO), stem cell factor (SCF), and hepatocyte growth factor (HGF). Representative data from one of at least three independent experiments is shown (B) qPCR of MST1R (RON) expression in HEL cells transduced with 4 different MST1R <t>shRNA</t> constructs (C) Colony formation assay of transduced HEL cells in methylcellulose with myeloid growth factors. Experiments were performed at least three times, with similar results. ANOVA with Tukey’s multiple-comparison test was used. (D) Western analysis of RON, STAT3 and STAT5 phosphorylation in shRNA-transduced HEL cells stimulated with EPO, SCF, and HGF. Representative data from one of three independent experiments are shown. (E) Western analysis of JAK2 in shRNA-transduced HEL cells stimulated with EPO, SCF, and HGF (F) Flow cytometry analysis of RON phosphorylation in MPN patient or control donor peripheral blood CD45lo population with ex vivo EPO and SCF stimulation (G) Schematic for JAK2-V617F-GFP retroviral bone marrow transplantation using bone marrow from Stk−/− (RON−/−) mice (H) Peripheral blood analysis of WT (N=8) or Stk−/− (N=9) JAK2-V617F mice over time. WBC= white blood cells; Hb=hemoglobin; PLT=platelets (I) Percentage of GFP in the peripheral blood over time in WT (N=8) or
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Figure 4: RON kinase is an important target of crizotinib in MPN (A) Western analysis of RON in SET2 cells treated with crizotinib and stimulated with erythropoietin (EPO), stem cell factor (SCF), and hepatocyte growth factor (HGF). Representative data from one of at least three independent experiments is shown (B) qPCR of MST1R (RON) expression in HEL cells transduced with 4 different MST1R shRNA constructs (C) Colony formation assay of transduced HEL cells in methylcellulose with myeloid growth factors. Experiments were performed at least three times, with similar results. ANOVA with Tukey’s multiple-comparison test was used. (D) Western analysis of RON, STAT3 and STAT5 phosphorylation in shRNA-transduced HEL cells stimulated with EPO, SCF, and HGF. Representative data from one of three independent experiments are shown. (E) Western analysis of JAK2 in shRNA-transduced HEL cells stimulated with EPO, SCF, and HGF (F) Flow cytometry analysis of RON phosphorylation in MPN patient or control donor peripheral blood CD45lo population with ex vivo EPO and SCF stimulation (G) Schematic for JAK2-V617F-GFP retroviral bone marrow transplantation using bone marrow from Stk−/− (RON−/−) mice (H) Peripheral blood analysis of WT (N=8) or Stk−/− (N=9) JAK2-V617F mice over time. WBC= white blood cells; Hb=hemoglobin; PLT=platelets (I) Percentage of GFP in the peripheral blood over time in WT (N=8) or

Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

Article Title: Crizotinib Has Preclinical Efficacy in Philadelphia-Negative Myeloproliferative Neoplasms.

doi: 10.1158/1078-0432.CCR-22-1763

Figure Lengend Snippet: Figure 4: RON kinase is an important target of crizotinib in MPN (A) Western analysis of RON in SET2 cells treated with crizotinib and stimulated with erythropoietin (EPO), stem cell factor (SCF), and hepatocyte growth factor (HGF). Representative data from one of at least three independent experiments is shown (B) qPCR of MST1R (RON) expression in HEL cells transduced with 4 different MST1R shRNA constructs (C) Colony formation assay of transduced HEL cells in methylcellulose with myeloid growth factors. Experiments were performed at least three times, with similar results. ANOVA with Tukey’s multiple-comparison test was used. (D) Western analysis of RON, STAT3 and STAT5 phosphorylation in shRNA-transduced HEL cells stimulated with EPO, SCF, and HGF. Representative data from one of three independent experiments are shown. (E) Western analysis of JAK2 in shRNA-transduced HEL cells stimulated with EPO, SCF, and HGF (F) Flow cytometry analysis of RON phosphorylation in MPN patient or control donor peripheral blood CD45lo population with ex vivo EPO and SCF stimulation (G) Schematic for JAK2-V617F-GFP retroviral bone marrow transplantation using bone marrow from Stk−/− (RON−/−) mice (H) Peripheral blood analysis of WT (N=8) or Stk−/− (N=9) JAK2-V617F mice over time. WBC= white blood cells; Hb=hemoglobin; PLT=platelets (I) Percentage of GFP in the peripheral blood over time in WT (N=8) or

Article Snippet: Lentiviral Production: Human shRNA plasmid kits for RON (Cat # TL320425) and MET (Cat #TL320418) were purchased from Origene.

Techniques: Western Blot, Expressing, Transduction, shRNA, Construct, Colony Assay, Comparison, Phospho-proteomics, Flow Cytometry, Control, Ex Vivo, Retroviral, Transplantation Assay