Journal: Genes

Article Title: A Comprehensive Review of Bioinformatics Tools for Genomic Biomarker Discovery Driving Precision Oncology

doi: 10.3390/genes15081036

Figure Lengend Snippet: Overview of sequencing technologies 2004–2022. Post-Sanger sequencing technologies, beginning with Roche 454 Pyrosequencing in 2004, Illumina HiSeq and MiSeq (2007), SOLiD (2008), PacBioSMRT (2009), DNBS: Helicos (2010), Ion Torrent (2011), in situ RNA sequencing (2013), ONT nanopore (2015), spatial transcriptomics (2016), GeoSeq (2017), Slide-Seq (2019), and Revio (2022), illustrate the continuous improvements contributing to the genomics. It explores the various NGS sequencing techniques that have expanded upon chain termination and polymerase chain reaction.

Article Snippet: For example, Roche/454 FLX Pyrosequencer was the first high-throughput screening platform used in 2004 [ , ].

Techniques: Sequencing, In Situ, RNA Sequencing Assay, Polymerase Chain Reaction

Journal: Microorganisms

Article Title: Changes in the Gastrointestinal Microbiota Induced by Proton Pump Inhibitors—A Review of Findings from Experimental Trials

doi: 10.3390/microorganisms12061110

Figure Lengend Snippet: Changes in the gastrointestinal microbiota induced by proton pump inhibitors in experimental trials.

Article Snippet: Prospective open-label trial. Compensated cirrhosis and controls , n = 30 Omeprazole 40 mg/day, 2 weeks 16S rRNA pyrosequencing of faecal samples Extraction NS Universal primers for bacteria Roche 454-pyrosequencing QIIME, RDP , Difference in β-diversity UniFrac (w) and Bray-Curtis , ↑ , - , - , - , Streptococcaceae (cirrhosis 0.0 → 8.9, controls 0.2 → 5.7) a , - , - , [ ] Bajaj 2014 .

Techniques: DNA Extraction, Sequencing, Extraction, Bacteria, Blocking Assay

Journal: Microorganisms

Article Title: Changes in the Gastrointestinal Microbiota Induced by Proton Pump Inhibitors—A Review of Findings from Experimental Trials

doi: 10.3390/microorganisms12061110

Figure Lengend Snippet: Changes in the gastrointestinal microbiota induced by proton pump inhibitors in experimental trials.

Article Snippet: Most studies used 16S rRNA sequencing performed on a MiSeq Illumina platform, while Roche 454-pyrosequencing was performed in three studies, and shotgun metagenomic sequencing was performed in one study.

Techniques: DNA Extraction, Sequencing, Extraction, Bacteria, Blocking Assay

Journal: Microorganisms

Article Title: Changes in the Gastrointestinal Microbiota Induced by Proton Pump Inhibitors—A Review of Findings from Experimental Trials

doi: 10.3390/microorganisms12061110

Figure Lengend Snippet: Changes in the gastrointestinal microbiota induced by proton pump inhibitors in experimental trials.

Article Snippet: Prospective open-label trial. Decompensated cirrhosis PPI withdrawal , n = 15 Omeprazole 40 mg/day, 2 weeks 16S rRNA pyrosequencing of faecal samples Roche 454-pyrosequencing Other parameters NS , Difference in β-diversity UniFrac (w) and Bray-Curtis , ↑ (after initiation) ↑ (before withdrawal) , - , - , - , Streptococcaceae Porphyromonadaceae Streptococcaceae Veillonellaceae Porphyromonadaceae e , , , [ ] Bajaj 2018 .

Techniques: DNA Extraction, Sequencing, Extraction, Bacteria, Blocking Assay