Journal: Virology Journal

Article Title: Reovirus resistance in tumors mediated by elevated ISG expression: overcoming therapeutic resistance via JAK/STAT pathway modulation

doi: 10.1186/s12985-026-03118-0

Figure Lengend Snippet: The Impact of JAK-STAT Signaling Pathway-Induced ISGs on Reovirus Replication and Oncolytic Activity. A Relative expression of OAS1/2/3 , ISG15 , and MX2 mRNA in HS and LS cells measured by RT-qPCR. B COC1/DDP cells were pretreated with RO8191 for 24 h, followed by treatment with 5 MOI reovirus for an additional 24 h. Subsequently, the relative expression of OAS1/2/3 , ISG15 , and MX2 mRNA in COC1/DDP cells was measured using RT-qPCR. C Quantification of viral RNA levels by RT-qPCR. SKOV3 and COC1/DDP cells were treated as described in ( B ). D Assessment of cell viability using the CCK-8 assay. SKOV3 and COC1/DDP cells were pretreated with RO8191 for 24 h, followed by treatment with 5 MOI reovirus for 48 h. Statistical analyses were performed using the Student’s t -test ( n = 3 per group; * p < 0.05, ** p < 0.01, *** p < 0.001). Data are presented as mean ± SD

Article Snippet: SKOV3 and COC1/DDP cells were pre-treated with 3 μM RO8191 (MCE, USA) or 10 μM IFN-alpha-IFNAR-IN-1 (MCE, USA) for 24 h, followed by exposure to 5 MOI reovirus for 48 h [ , ].

Techniques: Activity Assay, Expressing, Quantitative RT-PCR, CCK-8 Assay

Journal: Arteriosclerosis, Thrombosis, and Vascular Biology

Article Title: Valvular Leaflets Are Not Innocent Bystanders: Divergent Fibrotic Remodeling Accompanies Functional Mitral and Tricuspid Regurgitation

doi: 10.1161/ATVBAHA.125.324134

Figure Lengend Snippet: Elucidating the mechanism of valvular interstitial cell (VIC) subpopulation imbalance during functional tricuspid regurgitation (FTR) progression. A , Temporal differentiation trajectory curves of antifibrotic and profibrotic VIC subpopulations. B , Cell density curve plots of each antifibrotic VIC and profibrotic VIC subpopulation along pseudotime progression. C , Gene cluster heatmap and functional enrichment analysis before and after branched point 1. D , Temporal gene expression profiling of profibrotic and antifibrotic VIC markers. E , Analysis of IFN (interferon) signaling during VIC differentiation. F , Gene Set Enrichment Analysis of TGF (transforming growth factor) β signaling pathway enrichment with FTR disease severity. G , Comparison of IFITM1/3 expression between antifibrotic and profibrotic VICs (Student t test with Welch correction). H , Dot plot showing the IFN signaling intensity during the progression of FTR and functional mitral regurgitation (FMR). I , Immunofluorescence staining of IFITM1 in valve tissues from patients with different FMR stages; scale bar indicates 200 µm. J , Representative immunohistochemical images of the TGFβ signaling activation marker p-SMAD3 at different stages of FTR disease. DAPI indicates 4′,6-diamidino-2-phenylindole; DDRTree, Discriminative Dimensionality Reduction via learning a Tree; ECM, extracellular matrix; GO, gene ontology; IFN, interferon; NES, normalized enrichment score; and TV, tricuspid valve.

Article Snippet: Concurrently, RO8191, an IFN (interferon) receptor agonist (HY-W063968; MCE ), was used to examine the regulatory effects of IFN pathway activation on the phenotypic transition of valvular interstitial cells (VICs) from an antifibrotic to a profibrotic state.

Techniques: Functional Assay, Gene Expression, Comparison, Expressing, Immunofluorescence, Staining, Immunohistochemical staining, Activation Assay, Marker