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Relative abundance of root-associated fungi (RAF) based on ITS2 barcoding (A), raw counts of the metabolically active fungi based on <t>RNA</t> <t>sequencing</t> characterized by taxonomy (B), and Pearson correlations between DNA-based and RNA-based abundances of the fungal genera (C). RAF were studied on roots of European beech ( Fagus sylvatica ) grown in native forest soil and treated with either water (control), ammonium, or nitrate for 2 days before harvest ( n = 4 per treatment).
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Relative abundance of root-associated fungi (RAF) based on ITS2 barcoding (A), raw counts of the metabolically active fungi based on RNA sequencing characterized by taxonomy (B), and Pearson correlations between DNA-based and RNA-based abundances of the fungal genera (C). RAF were studied on roots of European beech ( Fagus sylvatica ) grown in native forest soil and treated with either water (control), ammonium, or nitrate for 2 days before harvest ( n = 4 per treatment).

Journal: mSystems

Article Title: Transcriptional Landscape of Ectomycorrhizal Fungi and Their Host Provides Insight into N Uptake from Forest Soil

doi: 10.1128/mSystems.00957-21

Figure Lengend Snippet: Relative abundance of root-associated fungi (RAF) based on ITS2 barcoding (A), raw counts of the metabolically active fungi based on RNA sequencing characterized by taxonomy (B), and Pearson correlations between DNA-based and RNA-based abundances of the fungal genera (C). RAF were studied on roots of European beech ( Fagus sylvatica ) grown in native forest soil and treated with either water (control), ammonium, or nitrate for 2 days before harvest ( n = 4 per treatment).

Article Snippet: RNA integrity check, library preparation, and sequencing were conducted at Chronix Biomedical GmbH (Göttingen, Germany).

Techniques: Metabolic Labelling, RNA Sequencing, Control