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Effect of the treatment with 600‐mg <t>rifampicin</t> or placebo orally once daily for 1 week on serum sex hormone–binding globulin (SHBG) levels in three clinical trials: (A) Rifa‐1 ( n = 12), (B) Rifa‐BP ( n = 22), (C) Rifa‐Stea ( n = 15) and (D) effect of the treatment with 600‐mg rifampicin orally once daily for 1 week on serum SHBG level compared to values before rifampicin dosing in Rifa‐2 clinical trial ( n = 12). (E) Results of all three cross‐over studies combined (Rifa‐1, Rifa‐BP, Rifa‐Stea; n = 49). (F–G) Correlation between plasma 4β‐hydroxycholesterol (4βHC) and serum SHBG in the rifampicin (F) and placebo (G) arms in healthy volunteers. Pearson correlation coefficients were calculated to analyse the correlation of 4βHC with SHBG levels. (H) Correlation between 4βHC and SHBG with rifampicin and placebo results combined.
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Effect of the treatment with 600‐mg rifampicin or placebo orally once daily for 1 week on serum sex hormone–binding globulin (SHBG) levels in three clinical trials: (A) Rifa‐1 ( n = 12), (B) Rifa‐BP ( n = 22), (C) Rifa‐Stea ( n = 15) and (D) effect of the treatment with 600‐mg rifampicin orally once daily for 1 week on serum SHBG level compared to values before rifampicin dosing in Rifa‐2 clinical trial ( n = 12). (E) Results of all three cross‐over studies combined (Rifa‐1, Rifa‐BP, Rifa‐Stea; n = 49). (F–G) Correlation between plasma 4β‐hydroxycholesterol (4βHC) and serum SHBG in the rifampicin (F) and placebo (G) arms in healthy volunteers. Pearson correlation coefficients were calculated to analyse the correlation of 4βHC with SHBG levels. (H) Correlation between 4βHC and SHBG with rifampicin and placebo results combined.

Journal: Basic & Clinical Pharmacology & Toxicology

Article Title: Pregnane X Receptor Regulates Human Endocrine System by Inducing Sex Hormone–Binding Globulin Expression

doi: 10.1111/bcpt.70218

Figure Lengend Snippet: Effect of the treatment with 600‐mg rifampicin or placebo orally once daily for 1 week on serum sex hormone–binding globulin (SHBG) levels in three clinical trials: (A) Rifa‐1 ( n = 12), (B) Rifa‐BP ( n = 22), (C) Rifa‐Stea ( n = 15) and (D) effect of the treatment with 600‐mg rifampicin orally once daily for 1 week on serum SHBG level compared to values before rifampicin dosing in Rifa‐2 clinical trial ( n = 12). (E) Results of all three cross‐over studies combined (Rifa‐1, Rifa‐BP, Rifa‐Stea; n = 49). (F–G) Correlation between plasma 4β‐hydroxycholesterol (4βHC) and serum SHBG in the rifampicin (F) and placebo (G) arms in healthy volunteers. Pearson correlation coefficients were calculated to analyse the correlation of 4βHC with SHBG levels. (H) Correlation between 4βHC and SHBG with rifampicin and placebo results combined.

Article Snippet: Rifampicin and SPA70 were purchased from Merck (St. Louis, Missouri, USA) and diluted in DMSO.

Techniques: Binding Assay, Clinical Proteomics

Regulation of SHBG expression in human 3D hepatocyte spheroids by rifampicin and SPA70 (both at 10 μM) as a function of time. (A) Effect of rifampicin on SHBG mRNA expression in three donors. (B) The mean effect of rifampicin in three donors. (C) Effect of SPA70 on SHBG mRNA expression in three donors. (D) The mean effect of SPA70 in three donors. (E) The hepatocyte spheroids were first treated with rifampicin or DMSO; after 72 h, SPA70 was added (mean effects in three donors).

Journal: Basic & Clinical Pharmacology & Toxicology

Article Title: Pregnane X Receptor Regulates Human Endocrine System by Inducing Sex Hormone–Binding Globulin Expression

doi: 10.1111/bcpt.70218

Figure Lengend Snippet: Regulation of SHBG expression in human 3D hepatocyte spheroids by rifampicin and SPA70 (both at 10 μM) as a function of time. (A) Effect of rifampicin on SHBG mRNA expression in three donors. (B) The mean effect of rifampicin in three donors. (C) Effect of SPA70 on SHBG mRNA expression in three donors. (D) The mean effect of SPA70 in three donors. (E) The hepatocyte spheroids were first treated with rifampicin or DMSO; after 72 h, SPA70 was added (mean effects in three donors).

Article Snippet: Rifampicin and SPA70 were purchased from Merck (St. Louis, Missouri, USA) and diluted in DMSO.

Techniques: Expressing