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Evaluation of C. difficile abundance A to C by qPCR targeting the toxA gene and D to F the associated toxin A/B levels measured with ELISA on feces samples from three different time points: before C. difficile inoculum, before 2nd treatment, and at study termination. The fraction below the boxplots highlights the number of mice that was detected positive of either C. difficile or toxin A/B. Dashed line marks the detection limit of the applied assay. * p < 0.05, ** p < 0.01

Journal: Microbiome

Article Title: Overcoming donor variability and risks associated with fecal microbiota transplants through bacteriophage-mediated treatments

doi: 10.1186/s40168-024-01820-1

Figure Lengend Snippet: Evaluation of C. difficile abundance A to C by qPCR targeting the toxA gene and D to F the associated toxin A/B levels measured with ELISA on feces samples from three different time points: before C. difficile inoculum, before 2nd treatment, and at study termination. The fraction below the boxplots highlights the number of mice that was detected positive of either C. difficile or toxin A/B. Dashed line marks the detection limit of the applied assay. * p < 0.05, ** p < 0.01

Article Snippet: The RIDASCREEN C. difficile Toxin A/B ELISA kit (R-Biopharm) was used to measure the toxin concentrations in the mice feces by following the instructions of the manufacturer.

Techniques: Enzyme-linked Immunosorbent Assay

Quantitative measurement of Shiga toxins (Stx1 and Stx2) by ELISA after APDBD treatment. Samples of Stx1 ( A ) and Stx2 ( B ) were treated with APDBD plasma for 0, 1, 2, 5, 15, and 30 min. Recovered samples after plasma treatment were subjected to an immunoassay using a RIDASCREEN ® Verotoxin kit (R-Biopharm AG, Darmstadt, Germany) to determine the concentrations of Stx1 and Stx2. Values were considered significantly different from the untreated sample (0 min) when verified by a non-repeated measures ANOVA, followed by a Tukey test (** p < 0.01).

Journal: International Journal of Molecular Sciences

Article Title: Degradation of Toxins Derived from Foodborne Pathogens by Atmospheric-Pressure Dielectric-Barrier Discharge

doi: 10.3390/ijms25115986

Figure Lengend Snippet: Quantitative measurement of Shiga toxins (Stx1 and Stx2) by ELISA after APDBD treatment. Samples of Stx1 ( A ) and Stx2 ( B ) were treated with APDBD plasma for 0, 1, 2, 5, 15, and 30 min. Recovered samples after plasma treatment were subjected to an immunoassay using a RIDASCREEN ® Verotoxin kit (R-Biopharm AG, Darmstadt, Germany) to determine the concentrations of Stx1 and Stx2. Values were considered significantly different from the untreated sample (0 min) when verified by a non-repeated measures ANOVA, followed by a Tukey test (** p < 0.01).

Article Snippet: A RIDASCREEN ® Verotoxin test kit and a RIDASCREEN ® SET Total kit were obtained from R-Biopharm AG (Darmstadt, Germany).

Techniques: Enzyme-linked Immunosorbent Assay

Quantitative measurement of Shiga toxins (Stx1 and Stx2) by ELISA after APDBD treatment. Samples of Stx1 ( A ) and Stx2 ( B ) were treated with APDBD plasma for 0, 1, 2, 5, 15, and 30 min. Recovered samples after plasma treatment were subjected to an immunoassay using a RIDASCREEN ® Verotoxin kit (R-Biopharm AG, Darmstadt, Germany) to determine the concentrations of Stx1 and Stx2. Values were considered significantly different from the untreated sample (0 min) when verified by a non-repeated measures ANOVA, followed by a Tukey test (** p < 0.01).

Journal: International Journal of Molecular Sciences

Article Title: Degradation of Toxins Derived from Foodborne Pathogens by Atmospheric-Pressure Dielectric-Barrier Discharge

doi: 10.3390/ijms25115986

Figure Lengend Snippet: Quantitative measurement of Shiga toxins (Stx1 and Stx2) by ELISA after APDBD treatment. Samples of Stx1 ( A ) and Stx2 ( B ) were treated with APDBD plasma for 0, 1, 2, 5, 15, and 30 min. Recovered samples after plasma treatment were subjected to an immunoassay using a RIDASCREEN ® Verotoxin kit (R-Biopharm AG, Darmstadt, Germany) to determine the concentrations of Stx1 and Stx2. Values were considered significantly different from the untreated sample (0 min) when verified by a non-repeated measures ANOVA, followed by a Tukey test (** p < 0.01).

Article Snippet: A RIDASCREEN ® Verotoxin test kit and a RIDASCREEN ® SET Total kit were obtained from R-Biopharm AG (Darmstadt, Germany).

Techniques: Enzyme-linked Immunosorbent Assay