Journal: Biomaterials Research

Article Title: Hybrid Dissolving Microneedles Incorporating Hyaluronic Acid Microdepots for Pain-free and Long-acting Corticosteroid Therapy

doi: 10.34133/bmr.0303

Figure Lengend Snippet: Characterization of TA@MDepots and hybrid DMN patches. (A) Comparison of average particle sizes of TA@MDepots before and after integration into hybrid DMNs. (B) Particle size distributions of TA@MDepots (left) and TA@MDepots extracted from hybrid DMN patches (right) are presented. (C) Shown are representative scanning electron microscopy (SEM) images of TA@MDepots. Scale bars: 10 μm. (D) Optical images depict hybrid DMN patches containing TA@MDepots (left) and RhoB@MDepots (right). Scale bars: 500 μm. (E) Representative SEM images display the top views of cross-sectioned microneedle tips loaded with microspheres. Scale bars: 100 μm. (F) Bright-field (left) and fluorescence (right) microscopic images show hybrid DMN patches containing RhoB@MDepots. Scale bar: 500 μm. (G) Representative SEM images illustrate the side view of a hybrid DMN patch loaded with TA@MDepots. Scale bars: 100 μm. (H) In vitro cumulative release profiles of TA from TA@MDepots, hybrid DMN patches, and the free-TA group ( n = 3) are shown. (I) Fourier transform infrared (FT-IR) spectra are presented for TA, acetylated hyaluronic acid (HA acetate), and TA@MDepots. (J) FT-IR spectra are also provided for TA@MDepots, polyvinyl alcohol (PVA), sucrose, and hybrid DMN patches. RhoB, Rhodamine B.

Article Snippet: Formamide, acetic anhydride, Rhodamine B (RhoB), acetonitrile (high-performance liquid chromatography [HPLC] grade), polyvinylpyrrolidone (K-30), and gelatin were all supplied by Daejung Chemicals (South Korea).

Techniques: Comparison, Electron Microscopy, Fluorescence, In Vitro, Fourier Transform Infrared Spectroscopy

Journal: Biomaterials Research

Article Title: Hybrid Dissolving Microneedles Incorporating Hyaluronic Acid Microdepots for Pain-free and Long-acting Corticosteroid Therapy

doi: 10.34133/bmr.0303

Figure Lengend Snippet: In vitro and ex vivo evaluation of skin insertion and mechanical properties of hybrid DMN patches. (A) Bright-field (left) and fluorescence (right) images of a gelatin block postapplication of hybrid DMN patches encapsulating RhoB@MDepots. Scale bars: 100 μm. (B) Optical (left) and fluorescence (right) images of porcine skin following ex vivo insertion of hybrid DMN patches loaded with RhoB@MDepots. Scale bars: 500 μm. (C) Histological section of porcine skin demonstrating localization of RhoB@MDepots after treatment with hybrid DMN patches. Scale bar: 100 μm. (D) Comparison of the mechanical strength between microdepot-free DMN patches and hybrid DMN patches with TA@MDepots using displacement–force analysis. (E) Representative images showing hybrid DMN patches before and after mechanical assessment. Scale bar: 500 μm.

Article Snippet: Formamide, acetic anhydride, Rhodamine B (RhoB), acetonitrile (high-performance liquid chromatography [HPLC] grade), polyvinylpyrrolidone (K-30), and gelatin were all supplied by Daejung Chemicals (South Korea).

Techniques: In Vitro, Ex Vivo, Fluorescence, Blocking Assay, Comparison

Journal: Biomaterials Research

Article Title: Hybrid Dissolving Microneedles Incorporating Hyaluronic Acid Microdepots for Pain-free and Long-acting Corticosteroid Therapy

doi: 10.34133/bmr.0303

Figure Lengend Snippet: In vivo evaluation of the retention and biodegradation of microdepots administered using hybrid DMN patches. (A) Representative photographs and fluorescence images of mouse dorsal skin after application of hybrid DMN patches containing RhoB@MDepots. Fluorescence imaging was performed at days 0, 1, 4, 7, and 9 following application. Scale bar: 5 mm. (B) Quantitative assessment of fluorescence intensity in the dorsal skin over time, presented as a percentage relative to the intensity at day 0 ( n = 5).

Article Snippet: Formamide, acetic anhydride, Rhodamine B (RhoB), acetonitrile (high-performance liquid chromatography [HPLC] grade), polyvinylpyrrolidone (K-30), and gelatin were all supplied by Daejung Chemicals (South Korea).

Techniques: In Vivo, Fluorescence, Imaging