Journal: Blood Advances

Article Title: Rh alloimmunization in chronically transfused patients with thalassemia receiving RhD, C, E, and K matched transfusions

doi: 10.1182/bloodadvances.2020003732

Figure Lengend Snippet: Alloimmunization and genotype-predicted Rh antigen expression among chronically transfused patients with thalassemia receiving prophylactic C, E, and K matched red cells. (A) Antibody specificities detected among 40 chronically transfused patients with thalassemia. Columns for each specificity indicate patients’ corresponding antigen status (positive or negative) as reported by standard serologic typing methods. RHD and RHCE genotype-predicted Rh antigen expression among 5 Black (B) and 35 non-Black (C) patients with thalassemia. Partial antigens predicted from genotypes associated with alleles that result in Rh epitope(s) missing and absence of conventional antigen. RHD*DAU0 or RHCE*ce48C has not been shown to encode Rh proteins lacking epitopes and is considered altered antigens.

Article Snippet: RH genotyping was performed with RHD and RHCE BeadChip arrays (Bioarray/Immucor), and polymerase chain reaction–based assays, as described previously.

Techniques: Expressing

Journal: Blood Advances

Article Title: Rh alloimmunization in chronically transfused patients with thalassemia receiving RhD, C, E, and K matched transfusions

doi: 10.1182/bloodadvances.2020003732

Figure Lengend Snippet: Alloimmunization and genotype-predicted Rh antigen expression among chronically transfused patients with SCD receiving prophylactic C, E, and K matched red cells by simple transfusion. (A) Antibody specificities detected among 48 chronically transfused patients with SCD. Columns for each specificity indicate patients’ corresponding antigen status (positive or negative) as reported by standard serologic or genotyping methods. (B) RHD and RHCE genotype-predicted Rh antigen expression among patients with SCD. Partial antigens predicted from genotypes with variant alleles that result in Rh epitope(s) missing and absence of conventional antigen. RHD*DAU0 or RHCE*ce48C has not been shown to encode Rh proteins lacking epitopes and is considered altered antigens.

Article Snippet: RH genotyping was performed with RHD and RHCE BeadChip arrays (Bioarray/Immucor), and polymerase chain reaction–based assays, as described previously.

Techniques: Expressing, Variant Assay

Journal: The Journal of molecular diagnostics : JMD

Article Title: Validated Reference Panel from Renewable Source of Genomic DNA Available for Standardization of Blood Group Genotyping

doi: 10.1016/j.jmoldx.2019.02.003

Figure Lengend Snippet: Coverage of RBC Genotypes by the Entire Panel

Article Snippet: Collaborators used a wide variety of genotyping techniques, including droplet digital PCR, the human erythrocyte antigen, RHD, and RHCE BeadChip arrays from Immucor (Peachtree Corners, GA); HI-FI Blood by AXO Science (Villeurbanne, France); high-resolution melting analysis, ID-CORE XT, and BLOODchip REFERENCE by Progenika (Derio, Spain); matrix-assisted laser desorption/ionization-based assays, such as Hemo ID, from Agena Bioscience (San Diego, CA); next-generation sequencing, PCR-restriction fragment length polymorphism, PCR-SSP, either single or multiplex, RBC-Ready Gene, and RBC-FluoGene by inno-train Diagnostik GmbH (Kronberg im Taunus, Germany); real-time PCR-based assays; Sanger sequencing; and SNaPshot (Applied Biosystems).

Techniques: Sequencing

Journal: The Journal of molecular diagnostics : JMD

Article Title: Validated Reference Panel from Renewable Source of Genomic DNA Available for Standardization of Blood Group Genotyping

doi: 10.1016/j.jmoldx.2019.02.003

Figure Lengend Snippet: Genotypes of the 18 DNA Panel Members, Determined as Described in Materials and Methods

Article Snippet: Collaborators used a wide variety of genotyping techniques, including droplet digital PCR, the human erythrocyte antigen, RHD, and RHCE BeadChip arrays from Immucor (Peachtree Corners, GA); HI-FI Blood by AXO Science (Villeurbanne, France); high-resolution melting analysis, ID-CORE XT, and BLOODchip REFERENCE by Progenika (Derio, Spain); matrix-assisted laser desorption/ionization-based assays, such as Hemo ID, from Agena Bioscience (San Diego, CA); next-generation sequencing, PCR-restriction fragment length polymorphism, PCR-SSP, either single or multiplex, RBC-Ready Gene, and RBC-FluoGene by inno-train Diagnostik GmbH (Kronberg im Taunus, Germany); real-time PCR-based assays; Sanger sequencing; and SNaPshot (Applied Biosystems).

Techniques:

Journal: The Journal of molecular diagnostics : JMD

Article Title: Validated Reference Panel from Renewable Source of Genomic DNA Available for Standardization of Blood Group Genotyping

doi: 10.1016/j.jmoldx.2019.02.003

Figure Lengend Snippet: Assays Designed to Determine the Presence or Absence of a Target, and Not Zygosity

Article Snippet: Collaborators used a wide variety of genotyping techniques, including droplet digital PCR, the human erythrocyte antigen, RHD, and RHCE BeadChip arrays from Immucor (Peachtree Corners, GA); HI-FI Blood by AXO Science (Villeurbanne, France); high-resolution melting analysis, ID-CORE XT, and BLOODchip REFERENCE by Progenika (Derio, Spain); matrix-assisted laser desorption/ionization-based assays, such as Hemo ID, from Agena Bioscience (San Diego, CA); next-generation sequencing, PCR-restriction fragment length polymorphism, PCR-SSP, either single or multiplex, RBC-Ready Gene, and RBC-FluoGene by inno-train Diagnostik GmbH (Kronberg im Taunus, Germany); real-time PCR-based assays; Sanger sequencing; and SNaPshot (Applied Biosystems).

Techniques: