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Effects of Fks1 amino acid substitution on echinocandin susceptibility. Determined micafungin, anidulafungin, caspofungin, and <t>rezafungin</t> minimum inhibitory concentrations (MICs) as outlined by Clinical and Laboratory Standards Institute guidelines using broth microdilution. Wells are shaded to represent the percentage growth for each concentration compared to the growth control well. Threshold wells denoting the 50% growth inhibition compared to the growth control are marked “X” with corresponding MICs listed to the right.
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Effects of Fks1 amino acid substitution on echinocandin susceptibility. Determined micafungin, anidulafungin, caspofungin, and <t>rezafungin</t> minimum inhibitory concentrations (MICs) as outlined by Clinical and Laboratory Standards Institute guidelines using broth microdilution. Wells are shaded to represent the percentage growth for each concentration compared to the growth control well. Threshold wells denoting the 50% growth inhibition compared to the growth control are marked “X” with corresponding MICs listed to the right.
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Effects of Fks1 amino acid substitution on echinocandin susceptibility. Determined micafungin, anidulafungin, caspofungin, and <t>rezafungin</t> minimum inhibitory concentrations (MICs) as outlined by Clinical and Laboratory Standards Institute guidelines using broth microdilution. Wells are shaded to represent the percentage growth for each concentration compared to the growth control well. Threshold wells denoting the 50% growth inhibition compared to the growth control are marked “X” with corresponding MICs listed to the right.
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Effects of Fks1 amino acid substitution on echinocandin susceptibility. Determined micafungin, anidulafungin, caspofungin, and <t>rezafungin</t> minimum inhibitory concentrations (MICs) as outlined by Clinical and Laboratory Standards Institute guidelines using broth microdilution. Wells are shaded to represent the percentage growth for each concentration compared to the growth control well. Threshold wells denoting the 50% growth inhibition compared to the growth control are marked “X” with corresponding MICs listed to the right.
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Effects of Fks1 amino acid substitution on echinocandin susceptibility. Determined micafungin, anidulafungin, caspofungin, and <t>rezafungin</t> minimum inhibitory concentrations (MICs) as outlined by Clinical and Laboratory Standards Institute guidelines using broth microdilution. Wells are shaded to represent the percentage growth for each concentration compared to the growth control well. Threshold wells denoting the 50% growth inhibition compared to the growth control are marked “X” with corresponding MICs listed to the right.
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Effects of Fks1 amino acid substitution on echinocandin susceptibility. Determined micafungin, anidulafungin, caspofungin, and rezafungin minimum inhibitory concentrations (MICs) as outlined by Clinical and Laboratory Standards Institute guidelines using broth microdilution. Wells are shaded to represent the percentage growth for each concentration compared to the growth control well. Threshold wells denoting the 50% growth inhibition compared to the growth control are marked “X” with corresponding MICs listed to the right.

Journal: The Journal of Infectious Diseases

Article Title: A Candidozyma ( Candida ) auris– Optimized Episomal Plasmid–Induced Cas9-Editing System Reveals the Direct Impact of the S639F-Encoding FKS1 Mutation

doi: 10.1093/infdis/jiaf285

Figure Lengend Snippet: Effects of Fks1 amino acid substitution on echinocandin susceptibility. Determined micafungin, anidulafungin, caspofungin, and rezafungin minimum inhibitory concentrations (MICs) as outlined by Clinical and Laboratory Standards Institute guidelines using broth microdilution. Wells are shaded to represent the percentage growth for each concentration compared to the growth control well. Threshold wells denoting the 50% growth inhibition compared to the growth control are marked “X” with corresponding MICs listed to the right.

Article Snippet: Minimum inhibitory concentrations for anidulafungin (Sigma-Aldrich), caspofungin (Fisher Scientific), micafungin (Sigma-Aldrich), and rezafungin (MedChemExpress) were determined by broth microdilution in 96-well plates in accordance with the M27-A4 standard from the Clinical and Laboratory Standards Institute with minor modification as previously described [ , ].

Techniques: Concentration Assay, Control, Inhibition