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Comparative analysis of HEK 293T spheroid landscapes cultured in various media. ( A ) Comparative analysis of amino acid content across different culture media. Created with ChiPlot by Songshan Zhu (https://www.chiplot.online/index.html). ( B ) Comparative analysis of amino acid formulations in frequently reported culture media for spheroid research. ( C ) Image presentation of morphology and cell death in spheroids cultured in different media types. 3D imaging revealed spatial differences in spheroid size and morphology across culture systems. 3D remodeling of spheroids was conducted using <t>ReViSP</t> v2.2 software (https://sourceforge.net/projects/revisp/). Scale bar = 1 mm. ( D , F ) Violin plots illustrating the influence of different culture media on spheroid metrics ( D ) and ATP content ( F ) of HEK 293T spheroids. Each biological replicate was denoted by a distinct symbol ( N ≥ 3), with each symbol representing a technical replicate ( n = 8). ( E ) Dissecting and comparing fluorescent areas of spheroid death stained with propidium iodide across different culture media. RPMI 1640 increases cell death signals compared to other media, as evidenced by elevated PI fluorescence in necrotic regions. Scale bar = 1 mm.
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revisp  (MathWorks Inc)
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Comparative analysis of HEK 293T spheroid landscapes cultured in various media. ( A ) Comparative analysis of amino acid content across different culture media. Created with ChiPlot by Songshan Zhu (https://www.chiplot.online/index.html). ( B ) Comparative analysis of amino acid formulations in frequently reported culture media for spheroid research. ( C ) Image presentation of morphology and cell death in spheroids cultured in different media types. 3D imaging revealed spatial differences in spheroid size and morphology across culture systems. 3D remodeling of spheroids was conducted using <t>ReViSP</t> v2.2 software (https://sourceforge.net/projects/revisp/). Scale bar = 1 mm. ( D , F ) Violin plots illustrating the influence of different culture media on spheroid metrics ( D ) and ATP content ( F ) of HEK 293T spheroids. Each biological replicate was denoted by a distinct symbol ( N ≥ 3), with each symbol representing a technical replicate ( n = 8). ( E ) Dissecting and comparing fluorescent areas of spheroid death stained with propidium iodide across different culture media. RPMI 1640 increases cell death signals compared to other media, as evidenced by elevated PI fluorescence in necrotic regions. Scale bar = 1 mm.
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Comparative analysis of HEK 293T spheroid landscapes cultured in various media. ( A ) Comparative analysis of amino acid content across different culture media. Created with ChiPlot by Songshan Zhu (https://www.chiplot.online/index.html). ( B ) Comparative analysis of amino acid formulations in frequently reported culture media for spheroid research. ( C ) Image presentation of morphology and cell death in spheroids cultured in different media types. 3D imaging revealed spatial differences in spheroid size and morphology across culture systems. 3D remodeling of spheroids was conducted using <t>ReViSP</t> v2.2 software (https://sourceforge.net/projects/revisp/). Scale bar = 1 mm. ( D , F ) Violin plots illustrating the influence of different culture media on spheroid metrics ( D ) and ATP content ( F ) of HEK 293T spheroids. Each biological replicate was denoted by a distinct symbol ( N ≥ 3), with each symbol representing a technical replicate ( n = 8). ( E ) Dissecting and comparing fluorescent areas of spheroid death stained with propidium iodide across different culture media. RPMI 1640 increases cell death signals compared to other media, as evidenced by elevated PI fluorescence in necrotic regions. Scale bar = 1 mm.
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revisp tool  (MathWorks Inc)
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Comparative analysis of HEK 293T spheroid landscapes cultured in various media. ( A ) Comparative analysis of amino acid content across different culture media. Created with ChiPlot by Songshan Zhu (https://www.chiplot.online/index.html). ( B ) Comparative analysis of amino acid formulations in frequently reported culture media for spheroid research. ( C ) Image presentation of morphology and cell death in spheroids cultured in different media types. 3D imaging revealed spatial differences in spheroid size and morphology across culture systems. 3D remodeling of spheroids was conducted using <t>ReViSP</t> v2.2 software (https://sourceforge.net/projects/revisp/). Scale bar = 1 mm. ( D , F ) Violin plots illustrating the influence of different culture media on spheroid metrics ( D ) and ATP content ( F ) of HEK 293T spheroids. Each biological replicate was denoted by a distinct symbol ( N ≥ 3), with each symbol representing a technical replicate ( n = 8). ( E ) Dissecting and comparing fluorescent areas of spheroid death stained with propidium iodide across different culture media. RPMI 1640 increases cell death signals compared to other media, as evidenced by elevated PI fluorescence in necrotic regions. Scale bar = 1 mm.
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revisp software  (MathWorks Inc)
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Comparative analysis of HEK 293T spheroid landscapes cultured in various media. ( A ) Comparative analysis of amino acid content across different culture media. Created with ChiPlot by Songshan Zhu (https://www.chiplot.online/index.html). ( B ) Comparative analysis of amino acid formulations in frequently reported culture media for spheroid research. ( C ) Image presentation of morphology and cell death in spheroids cultured in different media types. 3D imaging revealed spatial differences in spheroid size and morphology across culture systems. 3D remodeling of spheroids was conducted using <t>ReViSP</t> v2.2 software (https://sourceforge.net/projects/revisp/). Scale bar = 1 mm. ( D , F ) Violin plots illustrating the influence of different culture media on spheroid metrics ( D ) and ATP content ( F ) of HEK 293T spheroids. Each biological replicate was denoted by a distinct symbol ( N ≥ 3), with each symbol representing a technical replicate ( n = 8). ( E ) Dissecting and comparing fluorescent areas of spheroid death stained with propidium iodide across different culture media. RPMI 1640 increases cell death signals compared to other media, as evidenced by elevated PI fluorescence in necrotic regions. Scale bar = 1 mm.
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Comparative analysis of HEK 293T spheroid landscapes cultured in various media. ( A ) Comparative analysis of amino acid content across different culture media. Created with ChiPlot by Songshan Zhu (https://www.chiplot.online/index.html). ( B ) Comparative analysis of amino acid formulations in frequently reported culture media for spheroid research. ( C ) Image presentation of morphology and cell death in spheroids cultured in different media types. 3D imaging revealed spatial differences in spheroid size and morphology across culture systems. 3D remodeling of spheroids was conducted using ReViSP v2.2 software (https://sourceforge.net/projects/revisp/). Scale bar = 1 mm. ( D , F ) Violin plots illustrating the influence of different culture media on spheroid metrics ( D ) and ATP content ( F ) of HEK 293T spheroids. Each biological replicate was denoted by a distinct symbol ( N ≥ 3), with each symbol representing a technical replicate ( n = 8). ( E ) Dissecting and comparing fluorescent areas of spheroid death stained with propidium iodide across different culture media. RPMI 1640 increases cell death signals compared to other media, as evidenced by elevated PI fluorescence in necrotic regions. Scale bar = 1 mm.

Journal: Scientific Reports

Article Title: Influence of experimental variables on spheroid attributes

doi: 10.1038/s41598-025-92037-1

Figure Lengend Snippet: Comparative analysis of HEK 293T spheroid landscapes cultured in various media. ( A ) Comparative analysis of amino acid content across different culture media. Created with ChiPlot by Songshan Zhu (https://www.chiplot.online/index.html). ( B ) Comparative analysis of amino acid formulations in frequently reported culture media for spheroid research. ( C ) Image presentation of morphology and cell death in spheroids cultured in different media types. 3D imaging revealed spatial differences in spheroid size and morphology across culture systems. 3D remodeling of spheroids was conducted using ReViSP v2.2 software (https://sourceforge.net/projects/revisp/). Scale bar = 1 mm. ( D , F ) Violin plots illustrating the influence of different culture media on spheroid metrics ( D ) and ATP content ( F ) of HEK 293T spheroids. Each biological replicate was denoted by a distinct symbol ( N ≥ 3), with each symbol representing a technical replicate ( n = 8). ( E ) Dissecting and comparing fluorescent areas of spheroid death stained with propidium iodide across different culture media. RPMI 1640 increases cell death signals compared to other media, as evidenced by elevated PI fluorescence in necrotic regions. Scale bar = 1 mm.

Article Snippet: 3D remodeling of spheroids was conducted using ReViSP v2.2 software (https://sourceforge.net/projects/revisp/).

Techniques: Cell Culture, Imaging, Software, Staining, Fluorescence