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SDS-PAGE of purified PSPO ( A ). Effect of varying concentration of FAD on the activity of PSPO ( B ). HPLC analysis showing the catalytic conversion of HMF to FDCA by PSPO ( C )

Journal: Biotechnology for Biofuels and Bioproducts

Article Title: Advance biocatalyst for the green conversion of 5-hydroxymethylfurfural to a biopolymer precursor 2,5-furandicarboxylic acid

doi: 10.1186/s13068-025-02705-7

Figure Lengend Snippet: SDS-PAGE of purified PSPO ( A ). Effect of varying concentration of FAD on the activity of PSPO ( B ). HPLC analysis showing the catalytic conversion of HMF to FDCA by PSPO ( C )

Article Snippet: Reaction samples were filtered using 0.2 μm filters, followed by analysis of HMF and its derivatives using reverse-phase HPLC equipped with an Aminex HPX-87H column (300*7.8 mm, BIO-RAD).

Techniques: SDS Page, Purification, Concentration Assay, Activity Assay