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a-f. Flow <t>cytometric</t> data for the rat age study are shown. Panel a = Change in body weight over the course of the treatment period; panel b = normalized liver weights; panel c = %MNHEP; panel d = %Ki-67-positive nuclei; panel e = hepatocyte proliferation index; panel f = %8n+ nuclei. For every graph, data for each individual rat is shown, and group means appear as green horizontal lines. Dunnett’s test results are shown to the far right of each graph, where statistically significant differences relative to the 6 week-old rat group appear as italicized black text as opposed to red text, and by grey circles as opposed to red circles. Circles’ diameters represent 95% confidence intervals.
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a-f. Flow <t>cytometric</t> data for the rat age study are shown. Panel a = Change in body weight over the course of the treatment period; panel b = normalized liver weights; panel c = %MNHEP; panel d = %Ki-67-positive nuclei; panel e = hepatocyte proliferation index; panel f = %8n+ nuclei. For every graph, data for each individual rat is shown, and group means appear as green horizontal lines. Dunnett’s test results are shown to the far right of each graph, where statistically significant differences relative to the 6 week-old rat group appear as italicized black text as opposed to red text, and by grey circles as opposed to red circles. Circles’ diameters represent 95% confidence intervals.
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a-f. Flow cytometric data for the rat age study are shown. Panel a = Change in body weight over the course of the treatment period; panel b = normalized liver weights; panel c = %MNHEP; panel d = %Ki-67-positive nuclei; panel e = hepatocyte proliferation index; panel f = %8n+ nuclei. For every graph, data for each individual rat is shown, and group means appear as green horizontal lines. Dunnett’s test results are shown to the far right of each graph, where statistically significant differences relative to the 6 week-old rat group appear as italicized black text as opposed to red text, and by grey circles as opposed to red circles. Circles’ diameters represent 95% confidence intervals.

Journal: Environmental and molecular mutagenesis

Article Title: Flow cytometric method for scoring rat liver micronuclei with simultaneous assessments of hepatocyte proliferation

doi: 10.1002/em.22168

Figure Lengend Snippet: a-f. Flow cytometric data for the rat age study are shown. Panel a = Change in body weight over the course of the treatment period; panel b = normalized liver weights; panel c = %MNHEP; panel d = %Ki-67-positive nuclei; panel e = hepatocyte proliferation index; panel f = %8n+ nuclei. For every graph, data for each individual rat is shown, and group means appear as green horizontal lines. Dunnett’s test results are shown to the far right of each graph, where statistically significant differences relative to the 6 week-old rat group appear as italicized black text as opposed to red text, and by grey circles as opposed to red circles. Circles’ diameters represent 95% confidence intervals.

Article Snippet: Heat-inactivated fetal bovine serum (FBS; cat. no. 89510-186) was from VWR, Radnor, PA. Reagents used for flow cytometric MN-RET scoring (Anticoagulant Solution, Buffer Solution, DNA Stain, Anti-CD71-FITC and Anti-CD61-PE Antibodies, RNase Solution, and Malaria Biostandards) were from In Vivo MicroFlow ® PLUS R Kits, Litron Laboratories, Rochester, NY.

Techniques:

a-f. Flow cytometric data for the 3 day diethylnitrosamine study are shown. Panel a = Change in body weight over the course of the treatment period; panel b = normalized liver weights; panel c = %MNHEP; panel d = %Ki-67-positive nuclei; panel e = hepatocyte proliferation index; panel f = %8n+ nuclei. For this study, low, mid, and high treatment groups correspond to 10, 20, and 40 mg/kg/day, respectively. For every graph, data for each individual rat is shown, and group means appear as horizontal green lines. Dunnett’s test results are shown to the far right of each graph, where statistically significant differences relative to the concurrent vehicle control group appear as italicized black text as opposed to red text, and by grey circles as opposed to red circles. Circles’ diameters represent 95% confidence intervals.

Journal: Environmental and molecular mutagenesis

Article Title: Flow cytometric method for scoring rat liver micronuclei with simultaneous assessments of hepatocyte proliferation

doi: 10.1002/em.22168

Figure Lengend Snippet: a-f. Flow cytometric data for the 3 day diethylnitrosamine study are shown. Panel a = Change in body weight over the course of the treatment period; panel b = normalized liver weights; panel c = %MNHEP; panel d = %Ki-67-positive nuclei; panel e = hepatocyte proliferation index; panel f = %8n+ nuclei. For this study, low, mid, and high treatment groups correspond to 10, 20, and 40 mg/kg/day, respectively. For every graph, data for each individual rat is shown, and group means appear as horizontal green lines. Dunnett’s test results are shown to the far right of each graph, where statistically significant differences relative to the concurrent vehicle control group appear as italicized black text as opposed to red text, and by grey circles as opposed to red circles. Circles’ diameters represent 95% confidence intervals.

Article Snippet: Heat-inactivated fetal bovine serum (FBS; cat. no. 89510-186) was from VWR, Radnor, PA. Reagents used for flow cytometric MN-RET scoring (Anticoagulant Solution, Buffer Solution, DNA Stain, Anti-CD71-FITC and Anti-CD61-PE Antibodies, RNase Solution, and Malaria Biostandards) were from In Vivo MicroFlow ® PLUS R Kits, Litron Laboratories, Rochester, NY.

Techniques:

a-f Flow cytometric data for the 14 day diethylnitrosamine study are shown. Panel a = Change in body weight over the course of the treatment period; panel b = normalized liver weights; panel c = %MNHEP; panel d = %Ki-67-positive nuclei; panel e = hepatocyte proliferation index; panel f = %8n+ nuclei. For this study, low, mid, and high treatment groups correspond to 5, 10, and 20 mg/kg/day, respectively. For every graph, data for each individual rat is shown, and group means appear as horizontal green lines. Dunnett’s test results are shown to the far right of each graph, where statistically significant differences relative to the concurrent vehicle control group appear as italicized black text as opposed to red text, and by grey circles as opposed to red circles. Circles’ diameters represent 95% confidence intervals.

Journal: Environmental and molecular mutagenesis

Article Title: Flow cytometric method for scoring rat liver micronuclei with simultaneous assessments of hepatocyte proliferation

doi: 10.1002/em.22168

Figure Lengend Snippet: a-f Flow cytometric data for the 14 day diethylnitrosamine study are shown. Panel a = Change in body weight over the course of the treatment period; panel b = normalized liver weights; panel c = %MNHEP; panel d = %Ki-67-positive nuclei; panel e = hepatocyte proliferation index; panel f = %8n+ nuclei. For this study, low, mid, and high treatment groups correspond to 5, 10, and 20 mg/kg/day, respectively. For every graph, data for each individual rat is shown, and group means appear as horizontal green lines. Dunnett’s test results are shown to the far right of each graph, where statistically significant differences relative to the concurrent vehicle control group appear as italicized black text as opposed to red text, and by grey circles as opposed to red circles. Circles’ diameters represent 95% confidence intervals.

Article Snippet: Heat-inactivated fetal bovine serum (FBS; cat. no. 89510-186) was from VWR, Radnor, PA. Reagents used for flow cytometric MN-RET scoring (Anticoagulant Solution, Buffer Solution, DNA Stain, Anti-CD71-FITC and Anti-CD61-PE Antibodies, RNase Solution, and Malaria Biostandards) were from In Vivo MicroFlow ® PLUS R Kits, Litron Laboratories, Rochester, NY.

Techniques:

a-f. Flow cytometric data for the 3 day quinoline study are shown. Panel a = Change in body weight over the course of the treatment period; panel b = normalized liver weights; panel c = %MNHEP; panel d = %Ki-67-positive nuclei; panel e = hepatocyte proliferation index; panel f = %8n+ nuclei. For this study, low, mid, and high treatment groups correspond to 75, 100, and 125 mg/kg/day, respectively. For every graph, data for each individual rat is shown, and group means appear as horizontal green lines. Dunnett’s test results are shown to the far right of each graph, where statistically significant differences relative to the concurrent vehicle control group appear as italicized black text as opposed to red text, and by grey circles as opposed to red circles. Circles’ diameters represent 95% confidence intervals.

Journal: Environmental and molecular mutagenesis

Article Title: Flow cytometric method for scoring rat liver micronuclei with simultaneous assessments of hepatocyte proliferation

doi: 10.1002/em.22168

Figure Lengend Snippet: a-f. Flow cytometric data for the 3 day quinoline study are shown. Panel a = Change in body weight over the course of the treatment period; panel b = normalized liver weights; panel c = %MNHEP; panel d = %Ki-67-positive nuclei; panel e = hepatocyte proliferation index; panel f = %8n+ nuclei. For this study, low, mid, and high treatment groups correspond to 75, 100, and 125 mg/kg/day, respectively. For every graph, data for each individual rat is shown, and group means appear as horizontal green lines. Dunnett’s test results are shown to the far right of each graph, where statistically significant differences relative to the concurrent vehicle control group appear as italicized black text as opposed to red text, and by grey circles as opposed to red circles. Circles’ diameters represent 95% confidence intervals.

Article Snippet: Heat-inactivated fetal bovine serum (FBS; cat. no. 89510-186) was from VWR, Radnor, PA. Reagents used for flow cytometric MN-RET scoring (Anticoagulant Solution, Buffer Solution, DNA Stain, Anti-CD71-FITC and Anti-CD61-PE Antibodies, RNase Solution, and Malaria Biostandards) were from In Vivo MicroFlow ® PLUS R Kits, Litron Laboratories, Rochester, NY.

Techniques:

a-f. Flow cytometric data for the 14 day quinoline study are shown. Panel a = Change in body weight over the course of the treatment period; panel b = normalized liver weights; panel c = %MNHEP; panel d = %Ki-67-positive nuclei; panel e = hepatocyte proliferation index; panel f = %8n+ nuclei. For this study, low, mid, and high treatment groups correspond to 50, 75, and 100 mg/kg/day, respectively. For every graph, data for each individual rat is shown, and group means appear as horizontal green lines. Dunnett’s test results are shown to the far right of each graph, where statistically significant differences relative to the concurrent vehicle control group appear as italicized black text as opposed to red text, and by grey circles as opposed to red circles. Circles’ diameters represent 95% confidence intervals.

Journal: Environmental and molecular mutagenesis

Article Title: Flow cytometric method for scoring rat liver micronuclei with simultaneous assessments of hepatocyte proliferation

doi: 10.1002/em.22168

Figure Lengend Snippet: a-f. Flow cytometric data for the 14 day quinoline study are shown. Panel a = Change in body weight over the course of the treatment period; panel b = normalized liver weights; panel c = %MNHEP; panel d = %Ki-67-positive nuclei; panel e = hepatocyte proliferation index; panel f = %8n+ nuclei. For this study, low, mid, and high treatment groups correspond to 50, 75, and 100 mg/kg/day, respectively. For every graph, data for each individual rat is shown, and group means appear as horizontal green lines. Dunnett’s test results are shown to the far right of each graph, where statistically significant differences relative to the concurrent vehicle control group appear as italicized black text as opposed to red text, and by grey circles as opposed to red circles. Circles’ diameters represent 95% confidence intervals.

Article Snippet: Heat-inactivated fetal bovine serum (FBS; cat. no. 89510-186) was from VWR, Radnor, PA. Reagents used for flow cytometric MN-RET scoring (Anticoagulant Solution, Buffer Solution, DNA Stain, Anti-CD71-FITC and Anti-CD61-PE Antibodies, RNase Solution, and Malaria Biostandards) were from In Vivo MicroFlow ® PLUS R Kits, Litron Laboratories, Rochester, NY.

Techniques: