Journal: Journal of Food Science and Technology

Article Title: Antibacterial efficacy of nisin, pediocin 34 and enterocin FH99 against L. monocytogenes, E. faecium and E. faecalis and bacteriocin cross resistance and antibiotic susceptibility of their bacteriocin resistant variants

doi: 10.1007/s13197-011-0500-3

Figure Lengend Snippet: Bacterial strains used in the study and culture conditions

Article Snippet: Table shows the culture conditions and the culture medium used for the bacterial cultures. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Bacteria Strains Culture Conditions Pediococcus pentosaceous 34 Bacteriocinogenic strain ; Lab. isolate 37 °C, MRS Enterococcus faecium FH99 Bacteriocinogenic strain ; Lab. isolate 37 °C, MRS Pediococcus acidilactici LB 42 (Indicator strain) 37 °C, MRS Enterococcus feacalis ATCC 29212 37 °C, BHI Listeria monocytogenes MTCC 657 37 °C, BHI Enterococcus faecium DSMZ 20477 37 °C, BHI Enterococcus faecium VRE Vancomycin Resistant Strain (VRE) Lab. isolate 37 °C, BHI Open in a separate window DSMZ Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (German Collection of Microorganisms and Cell Cultures) MTCC Microbial Type Culture Collection (Chandigarh, India) ATCC American Type Culture Collection BHI Brain Heart Infusion Broth MRS de Man Rogosa and Shrape Bacterial strains used in the study and culture conditions Bacteriocin solutions preparation One hundred milliliter aliquots of MRS broth (De Man et al. 1960 ) (pH 6.5) (HiMedia, Mumbai) were inoculated with active culture of E. faecium FH99 and Pediococcus pentosaceus 34 was inoculated (1%) and incubated at 37 °C for 24 h. Cell free culture supernatant (CFCS) were prepared by centrifugation of the cultures in refrigerated centrifuge at 10,000 rpm for 10 min.

Techniques:

Journal: Journal of Food Science and Technology

Article Title: Antibacterial efficacy of nisin, pediocin 34 and enterocin FH99 against L. monocytogenes, E. faecium and E. faecalis and bacteriocin cross resistance and antibiotic susceptibility of their bacteriocin resistant variants

doi: 10.1007/s13197-011-0500-3

Figure Lengend Snippet: Susceptibility of wild type strains and resistant variants to nisin, pediocin 34 and enterocin FH99 bacteriocins

Article Snippet: Table shows the culture conditions and the culture medium used for the bacterial cultures. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Bacteria Strains Culture Conditions Pediococcus pentosaceous 34 Bacteriocinogenic strain ; Lab. isolate 37 °C, MRS Enterococcus faecium FH99 Bacteriocinogenic strain ; Lab. isolate 37 °C, MRS Pediococcus acidilactici LB 42 (Indicator strain) 37 °C, MRS Enterococcus feacalis ATCC 29212 37 °C, BHI Listeria monocytogenes MTCC 657 37 °C, BHI Enterococcus faecium DSMZ 20477 37 °C, BHI Enterococcus faecium VRE Vancomycin Resistant Strain (VRE) Lab. isolate 37 °C, BHI Open in a separate window DSMZ Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (German Collection of Microorganisms and Cell Cultures) MTCC Microbial Type Culture Collection (Chandigarh, India) ATCC American Type Culture Collection BHI Brain Heart Infusion Broth MRS de Man Rogosa and Shrape Bacterial strains used in the study and culture conditions Bacteriocin solutions preparation One hundred milliliter aliquots of MRS broth (De Man et al. 1960 ) (pH 6.5) (HiMedia, Mumbai) were inoculated with active culture of E. faecium FH99 and Pediococcus pentosaceus 34 was inoculated (1%) and incubated at 37 °C for 24 h. Cell free culture supernatant (CFCS) were prepared by centrifugation of the cultures in refrigerated centrifuge at 10,000 rpm for 10 min.

Techniques:

Journal: Journal of Food Science and Technology

Article Title: Antibacterial efficacy of nisin, pediocin 34 and enterocin FH99 against L. monocytogenes, E. faecium and E. faecalis and bacteriocin cross resistance and antibiotic susceptibility of their bacteriocin resistant variants

doi: 10.1007/s13197-011-0500-3

Figure Lengend Snippet: MICs of nisin, pediocin 34 and enterocin FH99 bacteriocins

Article Snippet: Table shows the culture conditions and the culture medium used for the bacterial cultures. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Bacteria Strains Culture Conditions Pediococcus pentosaceous 34 Bacteriocinogenic strain ; Lab. isolate 37 °C, MRS Enterococcus faecium FH99 Bacteriocinogenic strain ; Lab. isolate 37 °C, MRS Pediococcus acidilactici LB 42 (Indicator strain) 37 °C, MRS Enterococcus feacalis ATCC 29212 37 °C, BHI Listeria monocytogenes MTCC 657 37 °C, BHI Enterococcus faecium DSMZ 20477 37 °C, BHI Enterococcus faecium VRE Vancomycin Resistant Strain (VRE) Lab. isolate 37 °C, BHI Open in a separate window DSMZ Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (German Collection of Microorganisms and Cell Cultures) MTCC Microbial Type Culture Collection (Chandigarh, India) ATCC American Type Culture Collection BHI Brain Heart Infusion Broth MRS de Man Rogosa and Shrape Bacterial strains used in the study and culture conditions Bacteriocin solutions preparation One hundred milliliter aliquots of MRS broth (De Man et al. 1960 ) (pH 6.5) (HiMedia, Mumbai) were inoculated with active culture of E. faecium FH99 and Pediococcus pentosaceus 34 was inoculated (1%) and incubated at 37 °C for 24 h. Cell free culture supernatant (CFCS) were prepared by centrifugation of the cultures in refrigerated centrifuge at 10,000 rpm for 10 min.

Techniques:

Journal: Journal of Food Science and Technology

Article Title: Antibacterial efficacy of nisin, pediocin 34 and enterocin FH99 against L. monocytogenes, E. faecium and E. faecalis and bacteriocin cross resistance and antibiotic susceptibility of their bacteriocin resistant variants

doi: 10.1007/s13197-011-0500-3

Figure Lengend Snippet: Concentrations of nisin, Pediocin 34 and Enterocin FH99 used alone and in different combinations to evaluate cell growth inhibition by bacteriocins

Article Snippet: Table shows the culture conditions and the culture medium used for the bacterial cultures. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Bacteria Strains Culture Conditions Pediococcus pentosaceous 34 Bacteriocinogenic strain ; Lab. isolate 37 °C, MRS Enterococcus faecium FH99 Bacteriocinogenic strain ; Lab. isolate 37 °C, MRS Pediococcus acidilactici LB 42 (Indicator strain) 37 °C, MRS Enterococcus feacalis ATCC 29212 37 °C, BHI Listeria monocytogenes MTCC 657 37 °C, BHI Enterococcus faecium DSMZ 20477 37 °C, BHI Enterococcus faecium VRE Vancomycin Resistant Strain (VRE) Lab. isolate 37 °C, BHI Open in a separate window DSMZ Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (German Collection of Microorganisms and Cell Cultures) MTCC Microbial Type Culture Collection (Chandigarh, India) ATCC American Type Culture Collection BHI Brain Heart Infusion Broth MRS de Man Rogosa and Shrape Bacterial strains used in the study and culture conditions Bacteriocin solutions preparation One hundred milliliter aliquots of MRS broth (De Man et al. 1960 ) (pH 6.5) (HiMedia, Mumbai) were inoculated with active culture of E. faecium FH99 and Pediococcus pentosaceus 34 was inoculated (1%) and incubated at 37 °C for 24 h. Cell free culture supernatant (CFCS) were prepared by centrifugation of the cultures in refrigerated centrifuge at 10,000 rpm for 10 min.

Techniques: Inhibition

Journal: Journal of Food Science and Technology

Article Title: Antibacterial efficacy of nisin, pediocin 34 and enterocin FH99 against L. monocytogenes, E. faecium and E. faecalis and bacteriocin cross resistance and antibiotic susceptibility of their bacteriocin resistant variants

doi: 10.1007/s13197-011-0500-3

Figure Lengend Snippet: Viable cell count (log cfu/ml) of L. monocytogenes and Enterococcus strains after treatment with nisin (N), pediocin 34 (P) and enterocin FH99 (E) alone and in different combinations

Article Snippet: Table shows the culture conditions and the culture medium used for the bacterial cultures. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Bacteria Strains Culture Conditions Pediococcus pentosaceous 34 Bacteriocinogenic strain ; Lab. isolate 37 °C, MRS Enterococcus faecium FH99 Bacteriocinogenic strain ; Lab. isolate 37 °C, MRS Pediococcus acidilactici LB 42 (Indicator strain) 37 °C, MRS Enterococcus feacalis ATCC 29212 37 °C, BHI Listeria monocytogenes MTCC 657 37 °C, BHI Enterococcus faecium DSMZ 20477 37 °C, BHI Enterococcus faecium VRE Vancomycin Resistant Strain (VRE) Lab. isolate 37 °C, BHI Open in a separate window DSMZ Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (German Collection of Microorganisms and Cell Cultures) MTCC Microbial Type Culture Collection (Chandigarh, India) ATCC American Type Culture Collection BHI Brain Heart Infusion Broth MRS de Man Rogosa and Shrape Bacterial strains used in the study and culture conditions Bacteriocin solutions preparation One hundred milliliter aliquots of MRS broth (De Man et al. 1960 ) (pH 6.5) (HiMedia, Mumbai) were inoculated with active culture of E. faecium FH99 and Pediococcus pentosaceus 34 was inoculated (1%) and incubated at 37 °C for 24 h. Cell free culture supernatant (CFCS) were prepared by centrifugation of the cultures in refrigerated centrifuge at 10,000 rpm for 10 min.

Techniques: Cell Counting, Incubation, Control

Journal: Journal of Food Science and Technology

Article Title: Antibacterial efficacy of nisin, pediocin 34 and enterocin FH99 against L. monocytogenes, E. faecium and E. faecalis and bacteriocin cross resistance and antibiotic susceptibility of their bacteriocin resistant variants

doi: 10.1007/s13197-011-0500-3

Figure Lengend Snippet: Susceptibility a of wild type strains and their bacteriocin resistant counterparts to antibiotics

Article Snippet: Table shows the culture conditions and the culture medium used for the bacterial cultures. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Bacteria Strains Culture Conditions Pediococcus pentosaceous 34 Bacteriocinogenic strain ; Lab. isolate 37 °C, MRS Enterococcus faecium FH99 Bacteriocinogenic strain ; Lab. isolate 37 °C, MRS Pediococcus acidilactici LB 42 (Indicator strain) 37 °C, MRS Enterococcus feacalis ATCC 29212 37 °C, BHI Listeria monocytogenes MTCC 657 37 °C, BHI Enterococcus faecium DSMZ 20477 37 °C, BHI Enterococcus faecium VRE Vancomycin Resistant Strain (VRE) Lab. isolate 37 °C, BHI Open in a separate window DSMZ Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (German Collection of Microorganisms and Cell Cultures) MTCC Microbial Type Culture Collection (Chandigarh, India) ATCC American Type Culture Collection BHI Brain Heart Infusion Broth MRS de Man Rogosa and Shrape Bacterial strains used in the study and culture conditions Bacteriocin solutions preparation One hundred milliliter aliquots of MRS broth (De Man et al. 1960 ) (pH 6.5) (HiMedia, Mumbai) were inoculated with active culture of E. faecium FH99 and Pediococcus pentosaceus 34 was inoculated (1%) and incubated at 37 °C for 24 h. Cell free culture supernatant (CFCS) were prepared by centrifugation of the cultures in refrigerated centrifuge at 10,000 rpm for 10 min.

Techniques:

Journal: Journal of Food Science and Technology

Article Title: Antibacterial efficacy of nisin, pediocin 34 and enterocin FH99 against L. monocytogenes, E. faecium and E. faecalis and bacteriocin cross resistance and antibiotic susceptibility of their bacteriocin resistant variants

doi: 10.1007/s13197-011-0500-3

Figure Lengend Snippet: Surface hydrophobicity * of the wild type (WT) (a) Listeria monocytogenes ATCC 53135, (b) L. monocytogenes MTCC 657 (c) E. faecium DSMZ 20477, (d) E. faecium VRE, (e) E. faecalis ATCC 29212 and their nisin resistant (Nr), Pediocin 34 resistant (Pr) and Enterocin FH99 resistant (Er) variants. *Values are presented as mean±SEM; n = 3. ¥, ψ Values with different superscripts differ significantly at the level of p < 0.001 between wild type and bacteriocin resistant strains

Article Snippet: Table shows the culture conditions and the culture medium used for the bacterial cultures. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Bacteria Strains Culture Conditions Pediococcus pentosaceous 34 Bacteriocinogenic strain ; Lab. isolate 37 °C, MRS Enterococcus faecium FH99 Bacteriocinogenic strain ; Lab. isolate 37 °C, MRS Pediococcus acidilactici LB 42 (Indicator strain) 37 °C, MRS Enterococcus feacalis ATCC 29212 37 °C, BHI Listeria monocytogenes MTCC 657 37 °C, BHI Enterococcus faecium DSMZ 20477 37 °C, BHI Enterococcus faecium VRE Vancomycin Resistant Strain (VRE) Lab. isolate 37 °C, BHI Open in a separate window DSMZ Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (German Collection of Microorganisms and Cell Cultures) MTCC Microbial Type Culture Collection (Chandigarh, India) ATCC American Type Culture Collection BHI Brain Heart Infusion Broth MRS de Man Rogosa and Shrape Bacterial strains used in the study and culture conditions Bacteriocin solutions preparation One hundred milliliter aliquots of MRS broth (De Man et al. 1960 ) (pH 6.5) (HiMedia, Mumbai) were inoculated with active culture of E. faecium FH99 and Pediococcus pentosaceus 34 was inoculated (1%) and incubated at 37 °C for 24 h. Cell free culture supernatant (CFCS) were prepared by centrifugation of the cultures in refrigerated centrifuge at 10,000 rpm for 10 min.

Techniques: