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Journal: International Journal of Molecular Medicine
Article Title: Remimazolam alleviates myocardial ischemia/reperfusion injury and inflammation via inhibition of the NLRP3/IL-1β pathway in mice
doi: 10.3892/ijmm.2025.5498
Figure Lengend Snippet: Rema alleviates LPS-induced release and increased expression levels of IL-1β, IL-6 and TNF-α in cultured Raw264.7 cells. (A) Experimental protocol using cultured Raw264.7 macrophages. (B) LPS induced alterations in the release of IL-1β, IL-6 and TNF-α in Raw264.7 cells, while treatment with Rema inhibited these LPS-mediated changes (n=3). (C) LPS induced alterations in the gene expression levels of IL-1β, IL-6 and TNF-α in Raw264.7 cells, while treatment with Rema inhibited these effects (n=3). (D) DAPA inhibited the LPS-induced release of IL-1β, IL-6 and TNF-α in Raw264.7 cells, while treatment with Rema did not affect the alterations induced by DAPA (n=3). *** P<0.001. DAPA, dapansutrile; LPS, lipopolysaccharide; ns, not significant; Rema, remimazolam; RT-qPCR, reverse transcription-quantitative PCR.
Article Snippet: Cells were cultured in DMEM without FBS and pre-treated with 100 μ g/ml
Techniques: Expressing, Cell Culture, Gene Expression, Quantitative RT-PCR, Reverse Transcription, Real-time Polymerase Chain Reaction