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A549 Cell Il 11 Release Assay, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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progesterone release  (ALPCO)
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Fabrication of gold nano-particle-coated channel slides, containing areas of Anti-PS and <t>progesterone</t> coatings (the ‘NGS Device’) and its effect on immobilising low-quality sperm on the surface. (a) Surface coating with PPOx and addition of GNP to achieve covalent immobilization of COOH-GNP, followed by a second application of PPOx; covalent immobilization of anti-PS antibody (200 µg/mL) onto the glass surface next to the inlet well, and electrostatic adsorption of progesterone onto the half of the glass surface next to the outlet well. (b) Sperm attachment on the channel segment coated with Anti-PS. (c) The surface coated with progesterone exhibited a few or no sperm attachment. (d) Proportion of neat and attached sperm labelled by Annexin v was quantified by fluorescent microscopy. (e) The proportion of sperm labeled with TUNEL was quantified by fluorescent microscopy. (f) Representative fluorescent microscope images of neat samples stained with TUNEL (green) and DAI (blue). (g) Representative fluorescent microscope images of attached sperm on the neat and attached-sperm on Anti-PS-GNP coated surface stained with TUNEL (green) and DAPI (blue). (h, i) Bio-conjugation of Anti-PS using protein G and its effect on the reduction of apoptotic and necrotic sperm populations among recovered sperm through coated channel slides. One-way ANOVA followed by post-hoc t-test was used to evaluate differences between treatment groups. *P ≤ 0.01 and **P ≤ 0.001.
Progesterone Release, supplied by ALPCO, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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statistical software release 11  (STATA Corporation)
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Fabrication of gold nano-particle-coated channel slides, containing areas of Anti-PS and <t>progesterone</t> coatings (the ‘NGS Device’) and its effect on immobilising low-quality sperm on the surface. (a) Surface coating with PPOx and addition of GNP to achieve covalent immobilization of COOH-GNP, followed by a second application of PPOx; covalent immobilization of anti-PS antibody (200 µg/mL) onto the glass surface next to the inlet well, and electrostatic adsorption of progesterone onto the half of the glass surface next to the outlet well. (b) Sperm attachment on the channel segment coated with Anti-PS. (c) The surface coated with progesterone exhibited a few or no sperm attachment. (d) Proportion of neat and attached sperm labelled by Annexin v was quantified by fluorescent microscopy. (e) The proportion of sperm labeled with TUNEL was quantified by fluorescent microscopy. (f) Representative fluorescent microscope images of neat samples stained with TUNEL (green) and DAI (blue). (g) Representative fluorescent microscope images of attached sperm on the neat and attached-sperm on Anti-PS-GNP coated surface stained with TUNEL (green) and DAPI (blue). (h, i) Bio-conjugation of Anti-PS using protein G and its effect on the reduction of apoptotic and necrotic sperm populations among recovered sperm through coated channel slides. One-way ANOVA followed by post-hoc t-test was used to evaluate differences between treatment groups. *P ≤ 0.01 and **P ≤ 0.001.
Statistical Software Release 11, supplied by STATA Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fabrication of gold nano-particle-coated channel slides, containing areas of Anti-PS and <t>progesterone</t> coatings (the ‘NGS Device’) and its effect on immobilising low-quality sperm on the surface. (a) Surface coating with PPOx and addition of GNP to achieve covalent immobilization of COOH-GNP, followed by a second application of PPOx; covalent immobilization of anti-PS antibody (200 µg/mL) onto the glass surface next to the inlet well, and electrostatic adsorption of progesterone onto the half of the glass surface next to the outlet well. (b) Sperm attachment on the channel segment coated with Anti-PS. (c) The surface coated with progesterone exhibited a few or no sperm attachment. (d) Proportion of neat and attached sperm labelled by Annexin v was quantified by fluorescent microscopy. (e) The proportion of sperm labeled with TUNEL was quantified by fluorescent microscopy. (f) Representative fluorescent microscope images of neat samples stained with TUNEL (green) and DAI (blue). (g) Representative fluorescent microscope images of attached sperm on the neat and attached-sperm on Anti-PS-GNP coated surface stained with TUNEL (green) and DAPI (blue). (h, i) Bio-conjugation of Anti-PS using protein G and its effect on the reduction of apoptotic and necrotic sperm populations among recovered sperm through coated channel slides. One-way ANOVA followed by post-hoc t-test was used to evaluate differences between treatment groups. *P ≤ 0.01 and **P ≤ 0.001.
Corticotropin Releasing Hormone Elisa Kit Cat. No. H288 11, supplied by Nanjing Jiancheng Bioengineering Research Institute Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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corticotropin-releasing hormone elisa kit cat. no. h288-11 - by Bioz Stars, 2026-05
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kousa dogwood release (cf37a3e5, 2024-12-11)  (RStudio)
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Fabrication of gold nano-particle-coated channel slides, containing areas of Anti-PS and <t>progesterone</t> coatings (the ‘NGS Device’) and its effect on immobilising low-quality sperm on the surface. (a) Surface coating with PPOx and addition of GNP to achieve covalent immobilization of COOH-GNP, followed by a second application of PPOx; covalent immobilization of anti-PS antibody (200 µg/mL) onto the glass surface next to the inlet well, and electrostatic adsorption of progesterone onto the half of the glass surface next to the outlet well. (b) Sperm attachment on the channel segment coated with Anti-PS. (c) The surface coated with progesterone exhibited a few or no sperm attachment. (d) Proportion of neat and attached sperm labelled by Annexin v was quantified by fluorescent microscopy. (e) The proportion of sperm labeled with TUNEL was quantified by fluorescent microscopy. (f) Representative fluorescent microscope images of neat samples stained with TUNEL (green) and DAI (blue). (g) Representative fluorescent microscope images of attached sperm on the neat and attached-sperm on Anti-PS-GNP coated surface stained with TUNEL (green) and DAPI (blue). (h, i) Bio-conjugation of Anti-PS using protein G and its effect on the reduction of apoptotic and necrotic sperm populations among recovered sperm through coated channel slides. One-way ANOVA followed by post-hoc t-test was used to evaluate differences between treatment groups. *P ≤ 0.01 and **P ≤ 0.001.
Kousa Dogwood Release (Cf37a3e5, 2024 12 11), supplied by RStudio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/kousa dogwood release (cf37a3e5, 2024-12-11)/product/RStudio
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kousa dogwood release (cf37a3e5, 2024-12-11) - by Bioz Stars, 2026-05
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Fabrication of gold nano-particle-coated channel slides, containing areas of Anti-PS and <t>progesterone</t> coatings (the ‘NGS Device’) and its effect on immobilising low-quality sperm on the surface. (a) Surface coating with PPOx and addition of GNP to achieve covalent immobilization of COOH-GNP, followed by a second application of PPOx; covalent immobilization of anti-PS antibody (200 µg/mL) onto the glass surface next to the inlet well, and electrostatic adsorption of progesterone onto the half of the glass surface next to the outlet well. (b) Sperm attachment on the channel segment coated with Anti-PS. (c) The surface coated with progesterone exhibited a few or no sperm attachment. (d) Proportion of neat and attached sperm labelled by Annexin v was quantified by fluorescent microscopy. (e) The proportion of sperm labeled with TUNEL was quantified by fluorescent microscopy. (f) Representative fluorescent microscope images of neat samples stained with TUNEL (green) and DAI (blue). (g) Representative fluorescent microscope images of attached sperm on the neat and attached-sperm on Anti-PS-GNP coated surface stained with TUNEL (green) and DAPI (blue). (h, i) Bio-conjugation of Anti-PS using protein G and its effect on the reduction of apoptotic and necrotic sperm populations among recovered sperm through coated channel slides. One-way ANOVA followed by post-hoc t-test was used to evaluate differences between treatment groups. *P ≤ 0.01 and **P ≤ 0.001.
“Kousa Dogwood” Release (Cf37a3e5, 2024 12 11), supplied by RStudio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/“kousa dogwood” release (cf37a3e5, 2024-12-11)/product/RStudio
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“kousa dogwood” release (cf37a3e5, 2024-12-11) - by Bioz Stars, 2026-05
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11 mg sustained-release tablet of tofacitinib  (Pfizer Inc)
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Fabrication of gold nano-particle-coated channel slides, containing areas of Anti-PS and <t>progesterone</t> coatings (the ‘NGS Device’) and its effect on immobilising low-quality sperm on the surface. (a) Surface coating with PPOx and addition of GNP to achieve covalent immobilization of COOH-GNP, followed by a second application of PPOx; covalent immobilization of anti-PS antibody (200 µg/mL) onto the glass surface next to the inlet well, and electrostatic adsorption of progesterone onto the half of the glass surface next to the outlet well. (b) Sperm attachment on the channel segment coated with Anti-PS. (c) The surface coated with progesterone exhibited a few or no sperm attachment. (d) Proportion of neat and attached sperm labelled by Annexin v was quantified by fluorescent microscopy. (e) The proportion of sperm labeled with TUNEL was quantified by fluorescent microscopy. (f) Representative fluorescent microscope images of neat samples stained with TUNEL (green) and DAI (blue). (g) Representative fluorescent microscope images of attached sperm on the neat and attached-sperm on Anti-PS-GNP coated surface stained with TUNEL (green) and DAPI (blue). (h, i) Bio-conjugation of Anti-PS using protein G and its effect on the reduction of apoptotic and necrotic sperm populations among recovered sperm through coated channel slides. One-way ANOVA followed by post-hoc t-test was used to evaluate differences between treatment groups. *P ≤ 0.01 and **P ≤ 0.001.
11 Mg Sustained Release Tablet Of Tofacitinib, supplied by Pfizer Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/11 mg sustained-release tablet of tofacitinib/product/Pfizer Inc
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11 mg sustained-release tablet of tofacitinib - by Bioz Stars, 2026-05
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scils lab version 2023a release 11 00  (Bruker Corporation)
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Fabrication of gold nano-particle-coated channel slides, containing areas of Anti-PS and <t>progesterone</t> coatings (the ‘NGS Device’) and its effect on immobilising low-quality sperm on the surface. (a) Surface coating with PPOx and addition of GNP to achieve covalent immobilization of COOH-GNP, followed by a second application of PPOx; covalent immobilization of anti-PS antibody (200 µg/mL) onto the glass surface next to the inlet well, and electrostatic adsorption of progesterone onto the half of the glass surface next to the outlet well. (b) Sperm attachment on the channel segment coated with Anti-PS. (c) The surface coated with progesterone exhibited a few or no sperm attachment. (d) Proportion of neat and attached sperm labelled by Annexin v was quantified by fluorescent microscopy. (e) The proportion of sperm labeled with TUNEL was quantified by fluorescent microscopy. (f) Representative fluorescent microscope images of neat samples stained with TUNEL (green) and DAI (blue). (g) Representative fluorescent microscope images of attached sperm on the neat and attached-sperm on Anti-PS-GNP coated surface stained with TUNEL (green) and DAPI (blue). (h, i) Bio-conjugation of Anti-PS using protein G and its effect on the reduction of apoptotic and necrotic sperm populations among recovered sperm through coated channel slides. One-way ANOVA followed by post-hoc t-test was used to evaluate differences between treatment groups. *P ≤ 0.01 and **P ≤ 0.001.
Scils Lab Version 2023a Release 11 00, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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scils lab version 2023a release 11 00 - by Bioz Stars, 2026-05
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stata package release 11 0  (STATA Corporation)
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STATA Corporation stata package release 11 0
Fabrication of gold nano-particle-coated channel slides, containing areas of Anti-PS and <t>progesterone</t> coatings (the ‘NGS Device’) and its effect on immobilising low-quality sperm on the surface. (a) Surface coating with PPOx and addition of GNP to achieve covalent immobilization of COOH-GNP, followed by a second application of PPOx; covalent immobilization of anti-PS antibody (200 µg/mL) onto the glass surface next to the inlet well, and electrostatic adsorption of progesterone onto the half of the glass surface next to the outlet well. (b) Sperm attachment on the channel segment coated with Anti-PS. (c) The surface coated with progesterone exhibited a few or no sperm attachment. (d) Proportion of neat and attached sperm labelled by Annexin v was quantified by fluorescent microscopy. (e) The proportion of sperm labeled with TUNEL was quantified by fluorescent microscopy. (f) Representative fluorescent microscope images of neat samples stained with TUNEL (green) and DAI (blue). (g) Representative fluorescent microscope images of attached sperm on the neat and attached-sperm on Anti-PS-GNP coated surface stained with TUNEL (green) and DAPI (blue). (h, i) Bio-conjugation of Anti-PS using protein G and its effect on the reduction of apoptotic and necrotic sperm populations among recovered sperm through coated channel slides. One-way ANOVA followed by post-hoc t-test was used to evaluate differences between treatment groups. *P ≤ 0.01 and **P ≤ 0.001.
Stata Package Release 11 0, supplied by STATA Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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stata package release 11 0 - by Bioz Stars, 2026-05
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Fabrication of gold nano-particle-coated channel slides, containing areas of Anti-PS and progesterone coatings (the ‘NGS Device’) and its effect on immobilising low-quality sperm on the surface. (a) Surface coating with PPOx and addition of GNP to achieve covalent immobilization of COOH-GNP, followed by a second application of PPOx; covalent immobilization of anti-PS antibody (200 µg/mL) onto the glass surface next to the inlet well, and electrostatic adsorption of progesterone onto the half of the glass surface next to the outlet well. (b) Sperm attachment on the channel segment coated with Anti-PS. (c) The surface coated with progesterone exhibited a few or no sperm attachment. (d) Proportion of neat and attached sperm labelled by Annexin v was quantified by fluorescent microscopy. (e) The proportion of sperm labeled with TUNEL was quantified by fluorescent microscopy. (f) Representative fluorescent microscope images of neat samples stained with TUNEL (green) and DAI (blue). (g) Representative fluorescent microscope images of attached sperm on the neat and attached-sperm on Anti-PS-GNP coated surface stained with TUNEL (green) and DAPI (blue). (h, i) Bio-conjugation of Anti-PS using protein G and its effect on the reduction of apoptotic and necrotic sperm populations among recovered sperm through coated channel slides. One-way ANOVA followed by post-hoc t-test was used to evaluate differences between treatment groups. *P ≤ 0.01 and **P ≤ 0.001.

Journal: bioRxiv

Article Title: A surface-engineered microfluidic device for antibody-mediated negative selection of high-quality sperm for assisted reproduction

doi: 10.1101/2025.09.02.673619

Figure Lengend Snippet: Fabrication of gold nano-particle-coated channel slides, containing areas of Anti-PS and progesterone coatings (the ‘NGS Device’) and its effect on immobilising low-quality sperm on the surface. (a) Surface coating with PPOx and addition of GNP to achieve covalent immobilization of COOH-GNP, followed by a second application of PPOx; covalent immobilization of anti-PS antibody (200 µg/mL) onto the glass surface next to the inlet well, and electrostatic adsorption of progesterone onto the half of the glass surface next to the outlet well. (b) Sperm attachment on the channel segment coated with Anti-PS. (c) The surface coated with progesterone exhibited a few or no sperm attachment. (d) Proportion of neat and attached sperm labelled by Annexin v was quantified by fluorescent microscopy. (e) The proportion of sperm labeled with TUNEL was quantified by fluorescent microscopy. (f) Representative fluorescent microscope images of neat samples stained with TUNEL (green) and DAI (blue). (g) Representative fluorescent microscope images of attached sperm on the neat and attached-sperm on Anti-PS-GNP coated surface stained with TUNEL (green) and DAPI (blue). (h, i) Bio-conjugation of Anti-PS using protein G and its effect on the reduction of apoptotic and necrotic sperm populations among recovered sperm through coated channel slides. One-way ANOVA followed by post-hoc t-test was used to evaluate differences between treatment groups. *P ≤ 0.01 and **P ≤ 0.001.

Article Snippet: Progesterone release into the culture medium was quantified using a competitive Progesterone ELISA kit (ALPCO, Cat. No. 11-PROHU-E01), according to the manufacturer’s instructions.

Techniques: Adsorption, Microscopy, Labeling, TUNEL Assay, Staining, Conjugation Assay