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Longitudinal image of villi to demonstrate the localization of the antimicrobial SBD-3 (red) in the epithelial cells of the small intestine. Blue (DAPI) indicates cell nuclei and green (Phallicidin) shows F-actin. Note the green microvillous, brush border on the apical side of the cells (magnification ×200; <t>deconvolution</t> via 5 iterations). Panel A is magnification ×1200, Panel B is magnification ×500, and Panel C is magnification ×500. White arrows indicate SBD-3 in the epithelium and subepithelial capillaries in the panel A and are associated with the lacteal and surrounding capillaries in panel C. Scale bar in panel B = 90 microns.
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Longitudinal image of villi to demonstrate the localization of the antimicrobial SBD-3 (red) in the epithelial cells of the small intestine. Blue (DAPI) indicates cell nuclei and green (Phallicidin) shows F-actin. Note the green microvillous, brush border on the apical side of the cells (magnification ×200; <t>deconvolution</t> via 5 iterations). Panel A is magnification ×1200, Panel B is magnification ×500, and Panel C is magnification ×500. White arrows indicate SBD-3 in the epithelium and subepithelial capillaries in the panel A and are associated with the lacteal and surrounding capillaries in panel C. Scale bar in panel B = 90 microns.
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Longitudinal image of villi to demonstrate the localization of the antimicrobial SBD-3 (red) in the epithelial cells of the small intestine. Blue (DAPI) indicates cell nuclei and green (Phallicidin) shows F-actin. Note the green microvillous, brush border on the apical side of the cells (magnification ×200; <t>deconvolution</t> via 5 iterations). Panel A is magnification ×1200, Panel B is magnification ×500, and Panel C is magnification ×500. White arrows indicate SBD-3 in the epithelium and subepithelial capillaries in the panel A and are associated with the lacteal and surrounding capillaries in panel C. Scale bar in panel B = 90 microns.
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Longitudinal image of villi to demonstrate the localization of the antimicrobial SBD-3 (red) in the epithelial cells of the small intestine. Blue (DAPI) indicates cell nuclei and green (Phallicidin) shows F-actin. Note the green microvillous, brush border on the apical side of the cells (magnification ×200; <t>deconvolution</t> via 5 iterations). Panel A is magnification ×1200, Panel B is magnification ×500, and Panel C is magnification ×500. White arrows indicate SBD-3 in the epithelium and subepithelial capillaries in the panel A and are associated with the lacteal and surrounding capillaries in panel C. Scale bar in panel B = 90 microns.
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Longitudinal image of villi to demonstrate the localization of the antimicrobial SBD-3 (red) in the epithelial cells of the small intestine. Blue (DAPI) indicates cell nuclei and green (Phallicidin) shows F-actin. Note the green microvillous, brush border on the apical side of the cells (magnification ×200; deconvolution via 5 iterations). Panel A is magnification ×1200, Panel B is magnification ×500, and Panel C is magnification ×500. White arrows indicate SBD-3 in the epithelium and subepithelial capillaries in the panel A and are associated with the lacteal and surrounding capillaries in panel C. Scale bar in panel B = 90 microns.

Journal: Eplasty

Article Title: Upregulation of Defensins in Burn Sheep Small Intestine

doi:

Figure Lengend Snippet: Longitudinal image of villi to demonstrate the localization of the antimicrobial SBD-3 (red) in the epithelial cells of the small intestine. Blue (DAPI) indicates cell nuclei and green (Phallicidin) shows F-actin. Note the green microvillous, brush border on the apical side of the cells (magnification ×200; deconvolution via 5 iterations). Panel A is magnification ×1200, Panel B is magnification ×500, and Panel C is magnification ×500. White arrows indicate SBD-3 in the epithelium and subepithelial capillaries in the panel A and are associated with the lacteal and surrounding capillaries in panel C. Scale bar in panel B = 90 microns.

Article Snippet: Deconvolution and image analysis were performed by transferring the data sets to a Linux/RedHat workstation employing SoftWoRx software (Applied Precision), which uses an algorithm experimentally produced on the system from the convolution of a point spread function (PSF) to differentiate and reduce extraneous light or scattered light captured by the camera.

Techniques: