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96
World Precision Instruments cell patch clamp recordings
A) Schematic depicting JONs projecting through the antennal nerve and synapsing onto APN2 cells (gold dashed box) in the ipsilateral hemisphere of the central brain. Inset shows expression pattern of the genetic driver line labeling APN2 ( 24C06-GAL4) . Scale bar is 20 µm. B) During whole <t>cell</t> <t>patch</t> <t>clamp</t> <t>recordings,</t> antennal movements were recorded by a lateral camera and flight activity was monitored using an optical wingbeat detector. C) Example video frame with 2 tracked points and the relative direction of antenna angle deflections. Deflections down towards the head are represented as negative values, and deflections up and away from the head as positive values. D) Example single-trial traces showing antennal and APN2 activity during quiescence (left) and flight (right). Wing movement was detected using an infrared light sensor. E) Each dot is the average response of one APN2 cell. Black bars indicate the average across APN2 cells for each condition, with respective SEM error bars. Grey dashed lines pair measurements from the same cell during flight and quiescence. Across flies, the average APN2 membrane potential is reduced during bouts of flight compared to quiescence, both when the antennae are free (paired t-test; p = 0.037) and when the antennae are glued (Wilcoxon Signed-Rank Test; p = 0.014).
Cell Patch Clamp Recordings, supplied by World Precision Instruments, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Disease Registry electronic health record based sickle cell disease registry
A) Schematic depicting JONs projecting through the antennal nerve and synapsing onto APN2 cells (gold dashed box) in the ipsilateral hemisphere of the central brain. Inset shows expression pattern of the genetic driver line labeling APN2 ( 24C06-GAL4) . Scale bar is 20 µm. B) During whole <t>cell</t> <t>patch</t> <t>clamp</t> <t>recordings,</t> antennal movements were recorded by a lateral camera and flight activity was monitored using an optical wingbeat detector. C) Example video frame with 2 tracked points and the relative direction of antenna angle deflections. Deflections down towards the head are represented as negative values, and deflections up and away from the head as positive values. D) Example single-trial traces showing antennal and APN2 activity during quiescence (left) and flight (right). Wing movement was detected using an infrared light sensor. E) Each dot is the average response of one APN2 cell. Black bars indicate the average across APN2 cells for each condition, with respective SEM error bars. Grey dashed lines pair measurements from the same cell during flight and quiescence. Across flies, the average APN2 membrane potential is reduced during bouts of flight compared to quiescence, both when the antennae are free (paired t-test; p = 0.037) and when the antennae are glued (Wilcoxon Signed-Rank Test; p = 0.014).
Electronic Health Record Based Sickle Cell Disease Registry, supplied by Disease Registry, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Warner Instruments whole cell recordings
A) Schematic depicting JONs projecting through the antennal nerve and synapsing onto APN2 cells (gold dashed box) in the ipsilateral hemisphere of the central brain. Inset shows expression pattern of the genetic driver line labeling APN2 ( 24C06-GAL4) . Scale bar is 20 µm. B) During whole <t>cell</t> <t>patch</t> <t>clamp</t> <t>recordings,</t> antennal movements were recorded by a lateral camera and flight activity was monitored using an optical wingbeat detector. C) Example video frame with 2 tracked points and the relative direction of antenna angle deflections. Deflections down towards the head are represented as negative values, and deflections up and away from the head as positive values. D) Example single-trial traces showing antennal and APN2 activity during quiescence (left) and flight (right). Wing movement was detected using an infrared light sensor. E) Each dot is the average response of one APN2 cell. Black bars indicate the average across APN2 cells for each condition, with respective SEM error bars. Grey dashed lines pair measurements from the same cell during flight and quiescence. Across flies, the average APN2 membrane potential is reduced during bouts of flight compared to quiescence, both when the antennae are free (paired t-test; p = 0.037) and when the antennae are glued (Wilcoxon Signed-Rank Test; p = 0.014).
Whole Cell Recordings, supplied by Warner Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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97
Nikon whole cell patch clamp recordings
A) Schematic depicting JONs projecting through the antennal nerve and synapsing onto APN2 cells (gold dashed box) in the ipsilateral hemisphere of the central brain. Inset shows expression pattern of the genetic driver line labeling APN2 ( 24C06-GAL4) . Scale bar is 20 µm. B) During whole <t>cell</t> <t>patch</t> <t>clamp</t> <t>recordings,</t> antennal movements were recorded by a lateral camera and flight activity was monitored using an optical wingbeat detector. C) Example video frame with 2 tracked points and the relative direction of antenna angle deflections. Deflections down towards the head are represented as negative values, and deflections up and away from the head as positive values. D) Example single-trial traces showing antennal and APN2 activity during quiescence (left) and flight (right). Wing movement was detected using an infrared light sensor. E) Each dot is the average response of one APN2 cell. Black bars indicate the average across APN2 cells for each condition, with respective SEM error bars. Grey dashed lines pair measurements from the same cell during flight and quiescence. Across flies, the average APN2 membrane potential is reduced during bouts of flight compared to quiescence, both when the antennae are free (paired t-test; p = 0.037) and when the antennae are glued (Wilcoxon Signed-Rank Test; p = 0.014).
Whole Cell Patch Clamp Recordings, supplied by Nikon, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Jackson Laboratory cell patch clamp recordings
A) Schematic depicting JONs projecting through the antennal nerve and synapsing onto APN2 cells (gold dashed box) in the ipsilateral hemisphere of the central brain. Inset shows expression pattern of the genetic driver line labeling APN2 ( 24C06-GAL4) . Scale bar is 20 µm. B) During whole <t>cell</t> <t>patch</t> <t>clamp</t> <t>recordings,</t> antennal movements were recorded by a lateral camera and flight activity was monitored using an optical wingbeat detector. C) Example video frame with 2 tracked points and the relative direction of antenna angle deflections. Deflections down towards the head are represented as negative values, and deflections up and away from the head as positive values. D) Example single-trial traces showing antennal and APN2 activity during quiescence (left) and flight (right). Wing movement was detected using an infrared light sensor. E) Each dot is the average response of one APN2 cell. Black bars indicate the average across APN2 cells for each condition, with respective SEM error bars. Grey dashed lines pair measurements from the same cell during flight and quiescence. Across flies, the average APN2 membrane potential is reduced during bouts of flight compared to quiescence, both when the antennae are free (paired t-test; p = 0.037) and when the antennae are glued (Wilcoxon Signed-Rank Test; p = 0.014).
Cell Patch Clamp Recordings, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recording+cell/cell+clamp+patch+recordings+whole/pm41687612-167-1-11
Average 86 stars, based on 1 article reviews
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97
AutoMate Scientific Inc axopatch amplifier
A) Schematic depicting JONs projecting through the antennal nerve and synapsing onto APN2 cells (gold dashed box) in the ipsilateral hemisphere of the central brain. Inset shows expression pattern of the genetic driver line labeling APN2 ( 24C06-GAL4) . Scale bar is 20 µm. B) During whole <t>cell</t> <t>patch</t> <t>clamp</t> <t>recordings,</t> antennal movements were recorded by a lateral camera and flight activity was monitored using an optical wingbeat detector. C) Example video frame with 2 tracked points and the relative direction of antenna angle deflections. Deflections down towards the head are represented as negative values, and deflections up and away from the head as positive values. D) Example single-trial traces showing antennal and APN2 activity during quiescence (left) and flight (right). Wing movement was detected using an infrared light sensor. E) Each dot is the average response of one APN2 cell. Black bars indicate the average across APN2 cells for each condition, with respective SEM error bars. Grey dashed lines pair measurements from the same cell during flight and quiescence. Across flies, the average APN2 membrane potential is reduced during bouts of flight compared to quiescence, both when the antennae are free (paired t-test; p = 0.037) and when the antennae are glued (Wilcoxon Signed-Rank Test; p = 0.014).
Axopatch Amplifier, supplied by AutoMate Scientific Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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99
Nikon inverted microscope nikon ti s whole cell macroscopic current recordings
Testing inhibitory properties of SIL14 with patch-clamp. A Chemical structure of SIL14. B Representative current trace of pannexin-1 activity with 300 µM SIL14 <t>application.</t> <t>Whole-cell</t> recordings were obtained on CHO cells transiently transfected with mPanx1 under [90; −90] mV ramps. C Current–voltage characteristic of pannexin-1 conductance before and after SIL14 application. D Dose dependency of SIL14 effect on pannexin-1 measured at + 80 mV reveals IC 50 = 12.96 µM according to nonlinear regression analysis
Inverted Microscope Nikon Ti S Whole Cell Macroscopic Current Recordings, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Danaher Inc whole cell recording
Testing inhibitory properties of SIL14 with patch-clamp. A Chemical structure of SIL14. B Representative current trace of pannexin-1 activity with 300 µM SIL14 <t>application.</t> <t>Whole-cell</t> recordings were obtained on CHO cells transiently transfected with mPanx1 under [90; −90] mV ramps. C Current–voltage characteristic of pannexin-1 conductance before and after SIL14 application. D Dose dependency of SIL14 effect on pannexin-1 measured at + 80 mV reveals IC 50 = 12.96 µM according to nonlinear regression analysis
Whole Cell Recording, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


A) Schematic depicting JONs projecting through the antennal nerve and synapsing onto APN2 cells (gold dashed box) in the ipsilateral hemisphere of the central brain. Inset shows expression pattern of the genetic driver line labeling APN2 ( 24C06-GAL4) . Scale bar is 20 µm. B) During whole cell patch clamp recordings, antennal movements were recorded by a lateral camera and flight activity was monitored using an optical wingbeat detector. C) Example video frame with 2 tracked points and the relative direction of antenna angle deflections. Deflections down towards the head are represented as negative values, and deflections up and away from the head as positive values. D) Example single-trial traces showing antennal and APN2 activity during quiescence (left) and flight (right). Wing movement was detected using an infrared light sensor. E) Each dot is the average response of one APN2 cell. Black bars indicate the average across APN2 cells for each condition, with respective SEM error bars. Grey dashed lines pair measurements from the same cell during flight and quiescence. Across flies, the average APN2 membrane potential is reduced during bouts of flight compared to quiescence, both when the antennae are free (paired t-test; p = 0.037) and when the antennae are glued (Wilcoxon Signed-Rank Test; p = 0.014).

Journal: bioRxiv

Article Title: Sensory-motor integration in a nonspiking interneuron contributes to active sensor control in Drosophila

doi: 10.64898/2026.04.16.718965

Figure Lengend Snippet: A) Schematic depicting JONs projecting through the antennal nerve and synapsing onto APN2 cells (gold dashed box) in the ipsilateral hemisphere of the central brain. Inset shows expression pattern of the genetic driver line labeling APN2 ( 24C06-GAL4) . Scale bar is 20 µm. B) During whole cell patch clamp recordings, antennal movements were recorded by a lateral camera and flight activity was monitored using an optical wingbeat detector. C) Example video frame with 2 tracked points and the relative direction of antenna angle deflections. Deflections down towards the head are represented as negative values, and deflections up and away from the head as positive values. D) Example single-trial traces showing antennal and APN2 activity during quiescence (left) and flight (right). Wing movement was detected using an infrared light sensor. E) Each dot is the average response of one APN2 cell. Black bars indicate the average across APN2 cells for each condition, with respective SEM error bars. Grey dashed lines pair measurements from the same cell during flight and quiescence. Across flies, the average APN2 membrane potential is reduced during bouts of flight compared to quiescence, both when the antennae are free (paired t-test; p = 0.037) and when the antennae are glued (Wilcoxon Signed-Rank Test; p = 0.014).

Article Snippet: We used 6-11 MΩ thick-walled glass pipettes (Item #1B150F-3, World Precision Instruments) for whole cell patch clamp recordings, which we pulled using a Sutter P-1000 puller.

Techniques: Expressing, Labeling, Patch Clamp, Activity Assay, Membrane

Testing inhibitory properties of SIL14 with patch-clamp. A Chemical structure of SIL14. B Representative current trace of pannexin-1 activity with 300 µM SIL14 application. Whole-cell recordings were obtained on CHO cells transiently transfected with mPanx1 under [90; −90] mV ramps. C Current–voltage characteristic of pannexin-1 conductance before and after SIL14 application. D Dose dependency of SIL14 effect on pannexin-1 measured at + 80 mV reveals IC 50 = 12.96 µM according to nonlinear regression analysis

Journal: Purinergic Signalling

Article Title: Abnormal purinergic signaling contributes to development of renal cysts in autosomal dominant polycystic kidney disease

doi: 10.1007/s11302-025-10127-1

Figure Lengend Snippet: Testing inhibitory properties of SIL14 with patch-clamp. A Chemical structure of SIL14. B Representative current trace of pannexin-1 activity with 300 µM SIL14 application. Whole-cell recordings were obtained on CHO cells transiently transfected with mPanx1 under [90; −90] mV ramps. C Current–voltage characteristic of pannexin-1 conductance before and after SIL14 application. D Dose dependency of SIL14 effect on pannexin-1 measured at + 80 mV reveals IC 50 = 12.96 µM according to nonlinear regression analysis

Article Snippet: For each patch-clamp experiment, a glass chip with cells was transferred into the chamber with a constant flow of bath solution, placed under the inverted microscope Nikon Ti-S. Whole-cell macroscopic current recordings of pannexin-1 were made under voltage-clamp conditions with ramp protocol (90- > −90 mV).

Techniques: Patch Clamp, Activity Assay, Transfection