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In vivo animal studies.

Journal: EFSA Journal

Article Title: Scientific opinion on the extension of the authorisation of use of the food additive steviol glycosides (E 960a–d) and the modification of the acceptable daily intake (ADI) for steviol

doi: 10.2903/j.efsa.2024.9045

Figure Lengend Snippet: In vivo animal studies.

Article Snippet: Roberts and Renwick ( ) , Sprague Dawley Rats (male and female) , 14 C‐labelled Rebaudioside A, 14 C‐ stevioside and 14 C‐steviol (> 97% radiochem. purity) and unlabelled compounds , Rebaudioside A 5 mg/kg bw Stevioside 4.2 mg/kg bw Steviol 1.6 mg/kg bw Single dose by gavage , Plasma, urine, bile and faeces , LSC HPLC TLC , Plasma concentration‐ time profiles for Reb A and stevioside showed similar patterns for total radioactivity; C max and AUC (0–72h) were higher in female than in male rats The main metabolite in plasma was steviol and in lower amount steviol‐glucuronide Steviol was the main component in faeces , Rebaudioside A and stevioside are metabolised to steviol by gut microbioma and excreted in the faeces. Steviol‐glucuronide was present in the urine.

Techniques: In Vivo, Suspension, Radioactivity, Concentration Assay, Clinical Proteomics, Bacteria, Conjugation Assay, Sampling