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Grouping and treatment of rat peripheral blood lymphocyte proliferation detected using CCK-8 method. ( A ) The arrangement of the groups in the culture plates; ( B ) The order and procedure of treatment in each group.

Journal: Infection and Drug Resistance

Article Title: Experimental Research on the Effect of Tounong San on the Immune Function of Rats with Superficial Suppurative Infection

doi: 10.2147/IDR.S420199

Figure Lengend Snippet: Grouping and treatment of rat peripheral blood lymphocyte proliferation detected using CCK-8 method. ( A ) The arrangement of the groups in the culture plates; ( B ) The order and procedure of treatment in each group.

Article Snippet: Fluorescein isothiocyanate (FITC) (sigma, F7250), Concanavalin A (ConA) (sigma, c2010), lipopolysaccharide (LPS) (sigma, 0000081277), ethidium bromide (EB) (sigma, MKCJ7007); Dihydrorhodamine 123 (DHR 123) (MKbio, batch No.: 2509×200098), Phorbol 12-myristate 13-acetate (PMA) (MKbio, batch No.: 2208Z20915); rat peripheral blood lymphocyte separation solution kit, RPMI-1640 culture medium, hematoxylin-eosin stain kit (Beijing Solarbio Science & Technology Co., Ltd., batch No.: 20201010, 20201013, G1420); CCK-8 kit (Dojindo Beijing Co., Ltd., batch No.: SC675), soluble starch (Tianjin Kemao Chemical Products Co., Ltd., batch No.: 20200318); rat total complement (CH50) ELISA kit, rat interleukin-6 (IL-6) ELISA kit, rat interleukin-1 (IL-1) ELISA kit, rat tumor necrosis factor α (TNF-α) ELISA Kit, rat interferon γ (IFN- γ) ELISA kit, rat immunoglobulin M (IgM) ELISA kit, rat immunoglobulin G (IgG) ELISA Kit (the above ELISA Kits are produced by Wuhan Gene Beauty Biotechnology Co., Ltd., with the batch No. GR2020-12); anti-CD31 antibody [EPR17259] (Abcam, ab182981), anti-CD163 antibody [EPR19518] (Abcam, ab182422), anti-CD16 antibody [EPR16784] (Abcam, ab198507), anti-CD68 (Xavier, GB11067), endogenous peroxidase blocker (MaxVision, SPKIT-A2), animal non-immune serum (sheep, MaxVision, SPKIT-B1), HRP-Polymer anti-Rabbit IHC Kit (MaxVision Reagent, KIT-5005), HRP-Polymer anti-Mouse/Rabbit IHC Kit (MaxVision Reagent, KIT-5010), Staphylococcus aureus (China Academy of Food and Drug Control, CMCC 26003–5a25), cultivated as a third-generation working bacterium.

Techniques: CCK-8 Assay