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MedChemExpress
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Journal: Biomolecules
Article Title: Melatonin-Mediated Circadian Rhythm Signaling Exhibits Bidirectional Regulatory Effects on the State of Hair Follicle Stem Cells.
doi: 10.3390/biom15020226
Figure Lengend Snippet: Figure 1. (A) Results of ddPCR detection of surface markers of HFSCs in primary cultured cell models; blue dots represent positive signal microdroplets. (B) Immunofluorescence staining of CD34 and CD29 (surface markers of HFSCs) in primary cultured cell models; scale bar, 50 µm. (C) ddPCR detection of melatonin membrane receptor MT1 and MT2 expression in HFSCs. (D) Effects of different doses of melatonin on the transcriptional level of Rora. ns, not statistically significant; * p < 0.05. Subsequent significance markers use the same notation. (E) WB detection of effects of melatonin (1000 ng/L) on RORA levels. (F) Relative expression of RORA in HFSCs after melatonin (1000 ng/L) treatment.
Article Snippet: We treated the
Techniques: Cell Culture, Immunofluorescence, Staining, Membrane, Expressing
Journal: Regenerative Therapy
Article Title: Exosomes derived from platelet-rich plasma promote hair regeneration by regulating the SIRT1/FoxO3a pathway to alleviate oxidative stress
doi: 10.1016/j.reth.2025.08.005
Figure Lengend Snippet: PRP-exos treatment promotes proliferation and migration of HFSCs. (A) The morphology of PRP-exos was observed by TEM. (B) The exosomal markers were identified by Western blot. (C) Immunofluorescence was used to identify the marker CK19 of rat HFSCs. (D and E) Calcein/PI staining and CCK-8 were used to detect proliferation of HFSCs (n = 3). (F) Wound healing assays were used to measure the migration capacity of HFSCs (n = 3). ∗p < 0.05 compared with Control; ∗∗p < 0.01 compared with Control. Data are expressed as Mean ± SD.
Article Snippet: The
Techniques: Migration, Western Blot, Immunofluorescence, Marker, Staining, CCK-8 Assay, Control