Journal: Life

Article Title: Ascorbic Acid Neuroprotection Against Hippocampal Injury and Gliosis Induced by E621 in Albino Rats Through Modulation of GFAP, Synaptophysin, and Caspase-3

doi: 10.3390/life16081234

Figure Lengend Snippet: Representative photomicrographs of synaptophysin-immunostained sections in the DG of all studied groups. Synaptophysin appears minimal in the GL (arrow) of G1 and G2; however, it is significantly elevated, with robust, prominent cytoplasmic expression (arrows) in the GL of G3. In contrast, synaptophysin is significantly reduced in the GL (arrows) of G4 compared with G3. (synaptophysin immunostaining 400×).

Article Snippet: Sections were incubated overnight at 4 °C with primary antibodies against GFAP 1:100 (MS-280-B0; Lab Vision Corporation; Fremont, CA, USA), synaptophysin (A6344; Abclonal Technology; 1:100), and caspase-3 1:100 (PA1-29157; Thermo Fisher Scientific; Waltham, MA, USA).

Techniques: Expressing, Immunostaining

Journal: Life

Article Title: Ascorbic Acid Neuroprotection Against Hippocampal Injury and Gliosis Induced by E621 in Albino Rats Through Modulation of GFAP, Synaptophysin, and Caspase-3

doi: 10.3390/life16081234

Figure Lengend Snippet: M ± SEM of the percentage of degenerated neurons and the immunoreactivity of GFAP, synaptophysin, and activated caspase-3 in the DG region of the hippocampus across all groups.

Article Snippet: Sections were incubated overnight at 4 °C with primary antibodies against GFAP 1:100 (MS-280-B0; Lab Vision Corporation; Fremont, CA, USA), synaptophysin (A6344; Abclonal Technology; 1:100), and caspase-3 1:100 (PA1-29157; Thermo Fisher Scientific; Waltham, MA, USA).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Amyloid Precursor Protein Abnormalities Destabilize Membrane Ferroportin: A Novel Mechanism Underlying Early Brain Pathologies and Memory Impairment in Alzheimer’s Disease

doi: 10.3390/ijms27093892

Figure Lengend Snippet: FTP supplementation attenuated neuroinflammation, oxidative stress, and synaptic protein dysfunction in APP/PS1 mice. Representative western blot images of IL-6 and TNF-α ( A ), 4-HNE ( B ), PSD93 and synaptophysin ( C ) in the hippocampus of C57BL/6J + scramble, APP/PS1 + scramble, and APP/PS1 + FTP groups. Representative western blot images of IL-6 and TNF-α ( D ), 4-HNE ( E ), PSD93 and synaptophysin ( F ) in the cortex of C57BL/6J + scramble, APP/PS1 + scramble, and APP/PS1 + FTP groups. Quantification of IL-6, TNF-α, 4-HNE, PSD93, and synaptophysin expression were normalized to β-actin (fold change). Data are expressed as mean ± SEM ( n = 3 mice per group). * p < 0.05, ** p < 0.01, *** p < 0.001 (one-way ANOVA). ns (unpaired two-tailed Student’s t -test).

Article Snippet: Primary antibodies are described below: mouse anti-β-actin antibody (Boster Bio, Wuhan, China), rabbit anti-synaptophysin antibody (ABclonal, Wuhan, China), rabbit anti-PSD95 antibody (ABclonal, Wuhan, China), rabbit anti-4G8 antibody (Covance, Princeton, NJ, USA), FPN antibody (ABclonal, Wuhan, China), HMGB1 antibody (ABclonal, Wuhan, China), 4-HNE antibody (ABclonal, Wuhan, China), TfR antibody (Abcam, Cambridge, UK), GPX4 antibody (Abcam, Cambridge, UK), Thioflavin S (Shanghai Yuanye Bio-Technology Co., Ltd., Shanghai, China), Na/K + -ATPase (Abcam, Cambridge, UK).

Techniques: Western Blot, Expressing, Two Tailed Test