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The identified miRNAs in the fat bodies of silkworms. ( A ) the heat map between F_50 µM group and the control. ( B ) the differentially expressed miRNA between the F_50 µM group and the control. ( C ) the heat map between the F_200 µM group and the control. ( D ) the differentially expressed miRNA between the F_200 µM group and the control.

Journal: International Journal of Molecular Sciences

Article Title: MiRNA Omics Reveal the Mechanisms of the Dual Effects of Selenium Supplementation on the Development of the Silkworm ( Bombyx mori )

doi: 10.3390/ijms26073394

Figure Lengend Snippet: The identified miRNAs in the fat bodies of silkworms. ( A ) the heat map between F_50 µM group and the control. ( B ) the differentially expressed miRNA between the F_50 µM group and the control. ( C ) the heat map between the F_200 µM group and the control. ( D ) the differentially expressed miRNA between the F_200 µM group and the control.

Article Snippet: The detection of differentially expressed miRNAs was performed utilizing the miRNA fluorescence quantitative PCR kit (dye-based method) provided by Sangon Biotech (Shanghai, China) Co.

Techniques: Control

The validation of differentially expressed microRNAs (DE-miRNAs) was conducted using a reverse transcription quantitative polymerase chain reaction (RT-qPCR). The bars represent the relative expression levels of the microRNAs, with all values subjected to log2 transformation.

Journal: International Journal of Molecular Sciences

Article Title: MiRNA Omics Reveal the Mechanisms of the Dual Effects of Selenium Supplementation on the Development of the Silkworm ( Bombyx mori )

doi: 10.3390/ijms26073394

Figure Lengend Snippet: The validation of differentially expressed microRNAs (DE-miRNAs) was conducted using a reverse transcription quantitative polymerase chain reaction (RT-qPCR). The bars represent the relative expression levels of the microRNAs, with all values subjected to log2 transformation.

Article Snippet: The detection of differentially expressed miRNAs was performed utilizing the miRNA fluorescence quantitative PCR kit (dye-based method) provided by Sangon Biotech (Shanghai, China) Co.

Techniques: Biomarker Discovery, Reverse Transcription, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Expressing, Transformation Assay

miRNA-target interaction network. ( A ) The network of miRNAs and their targets in the Toll and Imd signaling pathway of the F_50 μM group; ( B ) the network of miRNAs and their targets in the spliceosome pathway of the F_200 μM group. Red color indicates significantly up-regulated miRNAs ( p < 0.01), green color indicates significantly down-regulated miRNAs ( p < 0.01), and blue color indicates targets.

Journal: International Journal of Molecular Sciences

Article Title: MiRNA Omics Reveal the Mechanisms of the Dual Effects of Selenium Supplementation on the Development of the Silkworm ( Bombyx mori )

doi: 10.3390/ijms26073394

Figure Lengend Snippet: miRNA-target interaction network. ( A ) The network of miRNAs and their targets in the Toll and Imd signaling pathway of the F_50 μM group; ( B ) the network of miRNAs and their targets in the spliceosome pathway of the F_200 μM group. Red color indicates significantly up-regulated miRNAs ( p < 0.01), green color indicates significantly down-regulated miRNAs ( p < 0.01), and blue color indicates targets.

Article Snippet: The detection of differentially expressed miRNAs was performed utilizing the miRNA fluorescence quantitative PCR kit (dye-based method) provided by Sangon Biotech (Shanghai, China) Co.

Techniques:

Construction of the KEGG-miRNA-targeting network. ( A ) Representative Toll and Imd signaling pathway regulatory networks in the F_50 μM group. ( B ) Representative spliceosome pathway regulatory network of the F_200 μM group. Triangles represent KEGG pathways, circles represent key miRNAs, and rectangles represent key targets. Red indicates obvious miRNAs. green indicates KEGG pathway. Blue indicates target gene.

Journal: International Journal of Molecular Sciences

Article Title: MiRNA Omics Reveal the Mechanisms of the Dual Effects of Selenium Supplementation on the Development of the Silkworm ( Bombyx mori )

doi: 10.3390/ijms26073394

Figure Lengend Snippet: Construction of the KEGG-miRNA-targeting network. ( A ) Representative Toll and Imd signaling pathway regulatory networks in the F_50 μM group. ( B ) Representative spliceosome pathway regulatory network of the F_200 μM group. Triangles represent KEGG pathways, circles represent key miRNAs, and rectangles represent key targets. Red indicates obvious miRNAs. green indicates KEGG pathway. Blue indicates target gene.

Article Snippet: The detection of differentially expressed miRNAs was performed utilizing the miRNA fluorescence quantitative PCR kit (dye-based method) provided by Sangon Biotech (Shanghai, China) Co.

Techniques: