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(A) <t>ALP</t> staining of rBMSCs cultured in DMEM with quercetin at different concentrations (0, 1, 2, 5 and 10 μM) for 10 days; (B) Quantitative analysis of <t>ALP</t> <t>activity</t> of rBMSCs cultured in DMEM with quercetin at different concentrations (0, 1, 2, 5 and 10 μM) for 4, 7 and 10 days. *p < 0.05 indicates quercetin-treated groups vs the control group; ▲ p < 0.05 indicates the 2 μM group vs the other quercetin groups.
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(A) ALP staining of rBMSCs cultured in DMEM with quercetin at different concentrations (0, 1, 2, 5 and 10 μM) for 10 days; (B) Quantitative analysis of ALP activity of rBMSCs cultured in DMEM with quercetin at different concentrations (0, 1, 2, 5 and 10 μM) for 4, 7 and 10 days. *p < 0.05 indicates quercetin-treated groups vs the control group; ▲ p < 0.05 indicates the 2 μM group vs the other quercetin groups.

Journal: PLoS ONE

Article Title: The Effect of Quercetin on the Osteogenesic Differentiation and Angiogenic Factor Expression of Bone Marrow-Derived Mesenchymal Stem Cells

doi: 10.1371/journal.pone.0129605

Figure Lengend Snippet: (A) ALP staining of rBMSCs cultured in DMEM with quercetin at different concentrations (0, 1, 2, 5 and 10 μM) for 10 days; (B) Quantitative analysis of ALP activity of rBMSCs cultured in DMEM with quercetin at different concentrations (0, 1, 2, 5 and 10 μM) for 4, 7 and 10 days. *p < 0.05 indicates quercetin-treated groups vs the control group; ▲ p < 0.05 indicates the 2 μM group vs the other quercetin groups.

Article Snippet: Moreover, at days 4, 7 and 10, ALP activity was quantitated following the manufacturer’s instructions (Beyotime, China).

Techniques: Staining, Cell Culture, Activity Assay, Control