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Pyrosequencing Inc pyrosequencing-based methylation analysis
Pyrosequencing Based Methylation Analysis, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pyrosequencing-based+methylation+analysis/pyrosequencing+based+methylation+analysis/pm40647502-43-0-0
Average 90 stars, based on 1 article reviews
pyrosequencing-based methylation analysis - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

DNA Methylation Assay:

Article Title: Metastasis-Specific CpG Island DNA Hypermethylation of the Long Non-Coding RNA Gene 00404 in Renal Cell Carcinoma.
Article Snippet: Pyrosequencing-based methylation analysis was conducted using negative and positive controls, as well as human primary and tumor cell line models, as previously described [20].

Article Title: Longitudinal increases in somatic mosaicism of the expanded CTG repeat in myotonic dystrophy type 1 are associated with variation in age-at-onset.
Article Snippet: The levels shown correspond to the average methylation from 11 (for CTCF-1) and 6 (for CTCF-2) CpG sites analyzed by pyrosequencing-based methylation analysis (PMA).

Article Title: Frequent silencing of RASSF1A by DNA methylation in thymic neuroendocrine tumours.
Article Snippet: Objectives: Aberrant methylation of promoter CpG islands (CGIs) of tumour suppressor genes is a common epigenetic mechanism underlying cancer pathogenesis.. The methylation patterns of thymic tumours have not been studied in detail since such tumours are rare.. Herein, we sought to identify genes that could serve as epigenetic targets for thymic neuroendocrine tumour (NET) therapy.

Article Title: Promoter methylation levels of microRNA-124 in non-neoplastic rectal mucosa as a potential biomarker for ulcerative colitis-associated colorectal cancer in pediatric-onset patients.
Article Snippet: Purpose To determine the methylation level of the miR-124 promoter in non-neoplastic rectal mucosa of patients with pediatric-onset ulcerative colitis (UC) to predict UC-associated colorectal cancer (UC-CRC).. Methods Between 2005 and 2017, non-neoplastic rectal tissue specimens were collected from 86 patients with UC, including 13 patients with UC-CRC; cancer tissues were obtained from the latter group.. The methylation status of the miR-124 promoter was quantified using bisulfite pyrosequencing and compared between pediatricand adult-onset UC patients.

Article Title: A Dietary Pattern Associated with LINE-1 Methylation Alters the Risk of Developing Cervical Intraepithelial Neoplasia
Article Snippet: As described below, methylation of the L1 promoter (GenBank accession no.x58075) in PBMCs was investigated using a pyrosequencing-based methylation analysis.

Article Title: Longitudinal increases in somatic mosaicism of the expanded CTG repeat in myotonic dystrophy type 1 are associated with variation in age-at-onset.
Article Snippet: We performed a pyrosequencing-based methylation analysis (PMA) to interrogate multiple CpG sites upstream (11 CpG sites spanning CTCF-1) or downstream (six CpG sites spanning CTCF-2) of the CTG repeat (Fig. 4A), in order to determine the methylation levels and status in PBL DNA samples over time from all 43 DM1 patients.

Article Title: New Molecules in Type 2 Diabetes: Advancements, Challenges and Future Directions
Article Snippet: In human ventricular cardiac myoblasts exposed to high glucose for 1 week, the effects of empagliflozin (EMPA) on hyperglycemia-induced DNA methylation of NF-κB, SOD2, and IL-6 genes by pyrosequencing-based methylation analysis were investigated [ ].

Methylation:

Article Title: Metastasis-Specific CpG Island DNA Hypermethylation of the Long Non-Coding RNA Gene 00404 in Renal Cell Carcinoma.
Article Snippet: Pyrosequencing-based methylation analysis was conducted using negative and positive controls, as well as human primary and tumor cell line models, as previously described [20].

Article Title: Longitudinal increases in somatic mosaicism of the expanded CTG repeat in myotonic dystrophy type 1 are associated with variation in age-at-onset.
Article Snippet: The levels shown correspond to the average methylation from 11 (for CTCF-1) and 6 (for CTCF-2) CpG sites analyzed by pyrosequencing-based methylation analysis (PMA).

Article Title: Frequent silencing of RASSF1A by DNA methylation in thymic neuroendocrine tumours.
Article Snippet: Objectives: Aberrant methylation of promoter CpG islands (CGIs) of tumour suppressor genes is a common epigenetic mechanism underlying cancer pathogenesis.. The methylation patterns of thymic tumours have not been studied in detail since such tumours are rare.. Herein, we sought to identify genes that could serve as epigenetic targets for thymic neuroendocrine tumour (NET) therapy.

Article Title: Promoter methylation levels of microRNA-124 in non-neoplastic rectal mucosa as a potential biomarker for ulcerative colitis-associated colorectal cancer in pediatric-onset patients.
Article Snippet: Purpose To determine the methylation level of the miR-124 promoter in non-neoplastic rectal mucosa of patients with pediatric-onset ulcerative colitis (UC) to predict UC-associated colorectal cancer (UC-CRC).. Methods Between 2005 and 2017, non-neoplastic rectal tissue specimens were collected from 86 patients with UC, including 13 patients with UC-CRC; cancer tissues were obtained from the latter group.. The methylation status of the miR-124 promoter was quantified using bisulfite pyrosequencing and compared between pediatricand adult-onset UC patients.

Article Title: A Dietary Pattern Associated with LINE-1 Methylation Alters the Risk of Developing Cervical Intraepithelial Neoplasia
Article Snippet: As described below, methylation of the L1 promoter (GenBank accession no.x58075) in PBMCs was investigated using a pyrosequencing-based methylation analysis.

Article Title: Longitudinal increases in somatic mosaicism of the expanded CTG repeat in myotonic dystrophy type 1 are associated with variation in age-at-onset.
Article Snippet: We performed a pyrosequencing-based methylation analysis (PMA) to interrogate multiple CpG sites upstream (11 CpG sites spanning CTCF-1) or downstream (six CpG sites spanning CTCF-2) of the CTG repeat (Fig. 4A), in order to determine the methylation levels and status in PBL DNA samples over time from all 43 DM1 patients.

Article Title: New Molecules in Type 2 Diabetes: Advancements, Challenges and Future Directions
Article Snippet: In human ventricular cardiac myoblasts exposed to high glucose for 1 week, the effects of empagliflozin (EMPA) on hyperglycemia-induced DNA methylation of NF-κB, SOD2, and IL-6 genes by pyrosequencing-based methylation analysis were investigated [ ].

Biomarker Discovery:

Article Title: Metastasis-Specific CpG Island DNA Hypermethylation of the Long Non-Coding RNA Gene 00404 in Renal Cell Carcinoma.
Article Snippet: Pyrosequencing-based methylation analysis was conducted using negative and positive controls, as well as human primary and tumor cell line models, as previously described [20].

Article Title: Longitudinal increases in somatic mosaicism of the expanded CTG repeat in myotonic dystrophy type 1 are associated with variation in age-at-onset.
Article Snippet: The levels shown correspond to the average methylation from 11 (for CTCF-1) and 6 (for CTCF-2) CpG sites analyzed by pyrosequencing-based methylation analysis (PMA).

Article Title: Frequent silencing of RASSF1A by DNA methylation in thymic neuroendocrine tumours.
Article Snippet: Objectives: Aberrant methylation of promoter CpG islands (CGIs) of tumour suppressor genes is a common epigenetic mechanism underlying cancer pathogenesis.. The methylation patterns of thymic tumours have not been studied in detail since such tumours are rare.. Herein, we sought to identify genes that could serve as epigenetic targets for thymic neuroendocrine tumour (NET) therapy.

Article Title: Promoter methylation levels of microRNA-124 in non-neoplastic rectal mucosa as a potential biomarker for ulcerative colitis-associated colorectal cancer in pediatric-onset patients.
Article Snippet: Purpose To determine the methylation level of the miR-124 promoter in non-neoplastic rectal mucosa of patients with pediatric-onset ulcerative colitis (UC) to predict UC-associated colorectal cancer (UC-CRC).. Methods Between 2005 and 2017, non-neoplastic rectal tissue specimens were collected from 86 patients with UC, including 13 patients with UC-CRC; cancer tissues were obtained from the latter group.. The methylation status of the miR-124 promoter was quantified using bisulfite pyrosequencing and compared between pediatricand adult-onset UC patients.

Article Title: A Dietary Pattern Associated with LINE-1 Methylation Alters the Risk of Developing Cervical Intraepithelial Neoplasia
Article Snippet: As described below, methylation of the L1 promoter (GenBank accession no.x58075) in PBMCs was investigated using a pyrosequencing-based methylation analysis.

Article Title: Longitudinal increases in somatic mosaicism of the expanded CTG repeat in myotonic dystrophy type 1 are associated with variation in age-at-onset.
Article Snippet: We performed a pyrosequencing-based methylation analysis (PMA) to interrogate multiple CpG sites upstream (11 CpG sites spanning CTCF-1) or downstream (six CpG sites spanning CTCF-2) of the CTG repeat (Fig. 4A), in order to determine the methylation levels and status in PBL DNA samples over time from all 43 DM1 patients.

Article Title: New Molecules in Type 2 Diabetes: Advancements, Challenges and Future Directions
Article Snippet: In human ventricular cardiac myoblasts exposed to high glucose for 1 week, the effects of empagliflozin (EMPA) on hyperglycemia-induced DNA methylation of NF-κB, SOD2, and IL-6 genes by pyrosequencing-based methylation analysis were investigated [ ].



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(A) Diagram of the experimental design. The differences of the global methylation levels between donors, pre-HCT recipients and post-HCT recipients were assessed by <t>a</t> <t>pyrosequencing</t> based methylation assay of repetitive <t>DNA</t> elements (LINE1 and NBL2) in whole blood. (B) NBL2 ΔMet values between donors, pre-HCT recipients, and 1 month post-HCT recipients. (C) NBL2 ΔMet mean values between donors and recipients up to 12 months post-transplant. The dotted line marks the ΔMet mean value between donors and 1 month post-HCT recipients (ΔMet = 5.8450). During the follow up of the transplant, the mean values barely deviated from the initial post-HCT ΔMet. (D) LINE1 ΔMet values between donors, pre-HCT recipients and 1 month post-HCT recipients. (E) LINE1 ΔMet mean values between donors and recipients up to 12 months post-transplant. The dotted line marked the ΔMet mean value between donors and 1 month post-HCT recipients (ΔMet = 5.383).
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Image Search Results


(A) Diagram of the experimental design. The differences of the global methylation levels between donors, pre-HCT recipients and post-HCT recipients were assessed by a pyrosequencing based methylation assay of repetitive DNA elements (LINE1 and NBL2) in whole blood. (B) NBL2 ΔMet values between donors, pre-HCT recipients, and 1 month post-HCT recipients. (C) NBL2 ΔMet mean values between donors and recipients up to 12 months post-transplant. The dotted line marks the ΔMet mean value between donors and 1 month post-HCT recipients (ΔMet = 5.8450). During the follow up of the transplant, the mean values barely deviated from the initial post-HCT ΔMet. (D) LINE1 ΔMet values between donors, pre-HCT recipients and 1 month post-HCT recipients. (E) LINE1 ΔMet mean values between donors and recipients up to 12 months post-transplant. The dotted line marked the ΔMet mean value between donors and 1 month post-HCT recipients (ΔMet = 5.383).

Journal: PLoS ONE

Article Title: DNA Methylation Dynamics in Blood after Hematopoietic Cell Transplant

doi: 10.1371/journal.pone.0056931

Figure Lengend Snippet: (A) Diagram of the experimental design. The differences of the global methylation levels between donors, pre-HCT recipients and post-HCT recipients were assessed by a pyrosequencing based methylation assay of repetitive DNA elements (LINE1 and NBL2) in whole blood. (B) NBL2 ΔMet values between donors, pre-HCT recipients, and 1 month post-HCT recipients. (C) NBL2 ΔMet mean values between donors and recipients up to 12 months post-transplant. The dotted line marks the ΔMet mean value between donors and 1 month post-HCT recipients (ΔMet = 5.8450). During the follow up of the transplant, the mean values barely deviated from the initial post-HCT ΔMet. (D) LINE1 ΔMet values between donors, pre-HCT recipients and 1 month post-HCT recipients. (E) LINE1 ΔMet mean values between donors and recipients up to 12 months post-transplant. The dotted line marked the ΔMet mean value between donors and 1 month post-HCT recipients (ΔMet = 5.383).

Article Snippet: Pyrosequencing-based DNA methylation analysis is relatively inexpensive, is very sensitive, and provides an interesting alternative to protein and mRNA detection in blood samples, allowing the development of novel biomarkers in a large array of human diseases.

Techniques: Methylation

Scatter plots showing DNA methylation in donors versus post-transplant recipients 1 month and 6 months post-HCT. Red dots represent CpG sites with methylation values altered more than 20% relative to donor values.

Journal: PLoS ONE

Article Title: DNA Methylation Dynamics in Blood after Hematopoietic Cell Transplant

doi: 10.1371/journal.pone.0056931

Figure Lengend Snippet: Scatter plots showing DNA methylation in donors versus post-transplant recipients 1 month and 6 months post-HCT. Red dots represent CpG sites with methylation values altered more than 20% relative to donor values.

Article Snippet: Pyrosequencing-based DNA methylation analysis is relatively inexpensive, is very sensitive, and provides an interesting alternative to protein and mRNA detection in blood samples, allowing the development of novel biomarkers in a large array of human diseases.

Techniques: DNA Methylation Assay, Methylation

LINE-1 and  Alu   PMA  Assays.

Journal: PLoS ONE

Article Title: Genome-Wide Hypomethylation in Head and Neck Cancer Is More Pronounced in HPV-Negative Tumors and Is Associated with Genomic Instability

doi: 10.1371/journal.pone.0004941

Figure Lengend Snippet: LINE-1 and Alu PMA Assays.

Article Snippet: To assay global methylation levels, we adapted our Pyrosequencing-based Methylation Analysis (PMA) assay to assess methylation of repetitive LINE and SINE ( Alu ) elements, similar to genome-wide methylation assays reported previously .

Techniques: