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pyrosequencing-based methylation analysis  (Pyrosequencing Inc)

 
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    Pyrosequencing Inc pyrosequencing-based methylation analysis
    Pyrosequencing Based Methylation Analysis, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pyrosequencing+based+methylation+analysis/pyrosequencing+based+methylation+analysis/pm40647502-43-0-0
    Average 90 stars, based on 1 article reviews
    pyrosequencing-based methylation analysis - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Methylation:

    Article Title: Insulin resistant diabetes mellitus in SHORT syndrome: case report and literature review
    Article Snippet: Array comparative genomic hybridization using a catalog human array (1 × 1M format, ID G4447A) (Agilent Technologies, CA, USA) showed neither patho‐ genic nor significance-unknown copy-number variant. .. Pyrosequencing-based methylation analysis indicated no abnormal methylation pattern for SRS-related differen‐ tially methylated regions (DMRs) including H19/IGF2: IG-DMR and KCNQ1OT1:TSS-DMR on chromosome 11, MEST:alt-TSS-DMR and PEG10:TSS-DMR on chromosome 7, MEG3/DLK1:IG-DMR and MEG3:TSSDMR on chromosome 14, GNAS A/B:TSS-DMR on chromosome 20, and ZNF597:DMR on chromosome 16. .. We next performed whole exome sequencing (WES), using SureSelect Human All Exon V6 (Agilent Technologies).



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    (A) Diagram of the experimental design. The differences of the global methylation levels between donors, pre-HCT recipients and post-HCT recipients were assessed by <t>a</t> <t>pyrosequencing</t> based methylation assay of repetitive <t>DNA</t> elements (LINE1 and NBL2) in whole blood. (B) NBL2 ΔMet values between donors, pre-HCT recipients, and 1 month post-HCT recipients. (C) NBL2 ΔMet mean values between donors and recipients up to 12 months post-transplant. The dotted line marks the ΔMet mean value between donors and 1 month post-HCT recipients (ΔMet = 5.8450). During the follow up of the transplant, the mean values barely deviated from the initial post-HCT ΔMet. (D) LINE1 ΔMet values between donors, pre-HCT recipients and 1 month post-HCT recipients. (E) LINE1 ΔMet mean values between donors and recipients up to 12 months post-transplant. The dotted line marked the ΔMet mean value between donors and 1 month post-HCT recipients (ΔMet = 5.383).
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    (A) Diagram of the experimental design. The differences of the global methylation levels between donors, pre-HCT recipients and post-HCT recipients were assessed by a pyrosequencing based methylation assay of repetitive DNA elements (LINE1 and NBL2) in whole blood. (B) NBL2 ΔMet values between donors, pre-HCT recipients, and 1 month post-HCT recipients. (C) NBL2 ΔMet mean values between donors and recipients up to 12 months post-transplant. The dotted line marks the ΔMet mean value between donors and 1 month post-HCT recipients (ΔMet = 5.8450). During the follow up of the transplant, the mean values barely deviated from the initial post-HCT ΔMet. (D) LINE1 ΔMet values between donors, pre-HCT recipients and 1 month post-HCT recipients. (E) LINE1 ΔMet mean values between donors and recipients up to 12 months post-transplant. The dotted line marked the ΔMet mean value between donors and 1 month post-HCT recipients (ΔMet = 5.383).

    Journal: PLoS ONE

    Article Title: DNA Methylation Dynamics in Blood after Hematopoietic Cell Transplant

    doi: 10.1371/journal.pone.0056931

    Figure Lengend Snippet: (A) Diagram of the experimental design. The differences of the global methylation levels between donors, pre-HCT recipients and post-HCT recipients were assessed by a pyrosequencing based methylation assay of repetitive DNA elements (LINE1 and NBL2) in whole blood. (B) NBL2 ΔMet values between donors, pre-HCT recipients, and 1 month post-HCT recipients. (C) NBL2 ΔMet mean values between donors and recipients up to 12 months post-transplant. The dotted line marks the ΔMet mean value between donors and 1 month post-HCT recipients (ΔMet = 5.8450). During the follow up of the transplant, the mean values barely deviated from the initial post-HCT ΔMet. (D) LINE1 ΔMet values between donors, pre-HCT recipients and 1 month post-HCT recipients. (E) LINE1 ΔMet mean values between donors and recipients up to 12 months post-transplant. The dotted line marked the ΔMet mean value between donors and 1 month post-HCT recipients (ΔMet = 5.383).

    Article Snippet: Pyrosequencing-based DNA methylation analysis is relatively inexpensive, is very sensitive, and provides an interesting alternative to protein and mRNA detection in blood samples, allowing the development of novel biomarkers in a large array of human diseases.

    Techniques: Methylation

    Scatter plots showing DNA methylation in donors versus post-transplant recipients 1 month and 6 months post-HCT. Red dots represent CpG sites with methylation values altered more than 20% relative to donor values.

    Journal: PLoS ONE

    Article Title: DNA Methylation Dynamics in Blood after Hematopoietic Cell Transplant

    doi: 10.1371/journal.pone.0056931

    Figure Lengend Snippet: Scatter plots showing DNA methylation in donors versus post-transplant recipients 1 month and 6 months post-HCT. Red dots represent CpG sites with methylation values altered more than 20% relative to donor values.

    Article Snippet: Pyrosequencing-based DNA methylation analysis is relatively inexpensive, is very sensitive, and provides an interesting alternative to protein and mRNA detection in blood samples, allowing the development of novel biomarkers in a large array of human diseases.

    Techniques: DNA Methylation Assay, Methylation

    LINE-1 and Alu PMA Assays.

    Journal: PLoS ONE

    Article Title: Genome-Wide Hypomethylation in Head and Neck Cancer Is More Pronounced in HPV-Negative Tumors and Is Associated with Genomic Instability

    doi: 10.1371/journal.pone.0004941

    Figure Lengend Snippet: LINE-1 and Alu PMA Assays.

    Article Snippet: To assay global methylation levels, we adapted our Pyrosequencing-based Methylation Analysis (PMA) assay to assess methylation of repetitive LINE and SINE ( Alu ) elements, similar to genome-wide methylation assays reported previously .

    Techniques: