Journal: Clinical Epigenetics

Article Title: ZEB1 hypermethylation is associated with better prognosis in patients with colon cancer

doi: 10.1186/s13148-023-01605-7

Figure Lengend Snippet: A Ideograms showing the location of the gene regions analyzed by pyrosequencing (PyroSeq) for CDX2 , FRMD6 and ZEB1 genes, obtained from UCSC Genome Browser (Human GRCh37/hg19) . The transcription start sites (TSSs), and the location of the PCR and sequencing primers are displayed. CpGs sites are represented by vertical bars. B Average percentage of CpGs methylation values within CDX2 and ZEB1 genes, and CpG methylation value of FRMD6 gene of each non-neoplastic colon (N) and each tumor (T) obtained by PyroSeq. The horizontal line represents the mean of both series (* p < 0.05, *** p < 0.001)

Article Snippet: Fig. 1 A Ideograms showing the location of the gene regions analyzed by pyrosequencing (PyroSeq) for CDX2 , FRMD6 and ZEB1 genes, obtained from UCSC Genome Browser (Human GRCh37/hg19) .

Techniques: Sequencing, Methylation, CpG Methylation Assay

Journal: Methods and Protocols

Article Title: Campylobacter Phage Isolation and Characterization: What We Have Learned So Far

doi: 10.3390/mps2010018

Figure Lengend Snippet: Some characteristics of hitherto sequenced Campylobacter phages.

Article Snippet: Sequencing/ platform , Shotgun seq. DNA libraries , 454 FLX pyroseq. and PCR/ Sanger , 454 FLX pyroseq., and PCR/ Sanger , 454 FLX pyroseq. , 454 FLX pyroseq. , Fidelity System , 454 FLX pyroseq. , n.a. , 454 FLX pyroseq. , 454 FLX pyroseq. , 454 FLX pyroseq. , Illumina.

Techniques: Isolation, Sequencing

Journal: Frontiers in Molecular Neuroscience

Article Title: Sugar Consumption Produces Effects Similar to Early Life Stress Exposure on Hippocampal Markers of Neurogenesis and Stress Response

doi: 10.3389/fnmol.2015.00086

Figure Lengend Snippet: The effects of LN exposure and sucrose consumption post-weaning on hippocampal gene expression of glucocorticoid receptor (A), Neurod1 (B), and Akt3 (C) . Results are expressed as mean ± S.E.M; Two-way ANOVA followed by LSD, n = 7–8/group. Post-hoc analysis was performed when significant interaction between diet and LN was present. * p < 0.05 vs. control rats consuming the same diet (LN effect). † p < 0.05 vs. rats consuming chow (diet effect).

Article Snippet: The pyrosequencing primer was Neurod1 pyroseq; TTAGTTGTTGAGGTTGAG and the pyrosequencing sequence to analyse was YGYGGGYGGGGTYGTGAGCT.

Techniques: Gene Expression, Control

Journal: Frontiers in Molecular Neuroscience

Article Title: Sugar Consumption Produces Effects Similar to Early Life Stress Exposure on Hippocampal Markers of Neurogenesis and Stress Response

doi: 10.3389/fnmol.2015.00086

Figure Lengend Snippet: Percentage of DNA methylation at 4 CpG sites in the (A) GR and (B) Neurod1 promoter regions across Con, LN, Con-Sucrose, and LN-Sucrose groups . Results are expressed as mean ± S.E.M; Two-way ANOVA followed by LSD, n = 8–13/group. No significant interaction was observed between LN exposure and sucrose diet.

Article Snippet: The pyrosequencing primer was Neurod1 pyroseq; TTAGTTGTTGAGGTTGAG and the pyrosequencing sequence to analyse was YGYGGGYGGGGTYGTGAGCT.

Techniques: DNA Methylation Assay