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Analysis was performed in Avisoft SASLab Pro (version 5.2.09; Avisoft Bioacoustics) with the following settings: 1024 FFT length, 100% frame, Hamming window, 75% time window overlap, 15 and 500 kHz frequency cutoffs, amplitude threshold of −60 dB, and a hold time of 30ms (78). a) Male Pcdh10+/− mice showed significantly increased numbers of <t>USVs</t> relative to male WT littermates (effect of genotype p<0.001) over the 5min recording period on postnatal day 6. b) Female Pcdh10+/− mice also showed significantly increased numbers of USVs relative to female WT littermates (effect of genotype p<0.001) (Figure 2) over 5min.
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Analysis was performed in Avisoft SASLab Pro (version 5.2.09; Avisoft Bioacoustics) with the following settings: 1024 FFT length, 100% frame, Hamming window, 75% time window overlap, 15 and 500 kHz frequency cutoffs, amplitude threshold of −60 dB, and a hold time of 30ms (78). a) Male Pcdh10+/− mice showed significantly increased numbers of USVs relative to male WT littermates (effect of genotype p<0.001) over the 5min recording period on postnatal day 6. b) Female Pcdh10+/− mice also showed significantly increased numbers of USVs relative to female WT littermates (effect of genotype p<0.001) (Figure 2) over 5min.

Journal: Biological psychiatry

Article Title: SOCIABILITY DEFICITS AND ALTERED AMYGDALA CIRCUITS IN MICE LACKING Pcdh10 , AN AUTISM ASSOCIATED GENE

doi: 10.1016/j.biopsych.2016.06.008

Figure Lengend Snippet: Analysis was performed in Avisoft SASLab Pro (version 5.2.09; Avisoft Bioacoustics) with the following settings: 1024 FFT length, 100% frame, Hamming window, 75% time window overlap, 15 and 500 kHz frequency cutoffs, amplitude threshold of −60 dB, and a hold time of 30ms (78). a) Male Pcdh10+/− mice showed significantly increased numbers of USVs relative to male WT littermates (effect of genotype p<0.001) over the 5min recording period on postnatal day 6. b) Female Pcdh10+/− mice also showed significantly increased numbers of USVs relative to female WT littermates (effect of genotype p<0.001) (Figure 2) over 5min.

Article Snippet: Pup ultrasonic vocalizations Postnatal day 6 ( 35 ) male and female pups were separated from their mother and placed alone in a glass container (10 cm × 8 cm × 7 cm; open surface) containing clean bedding, which was placed in an open Styrofoam container (20 cm × 21 cm × 12 cm) inside a sound-attenuating cabinet (Med Associates, St. Albans, VT), for 5 min. Ultrasonic vocalizations were recoded using an UltraSoundGate Condenser Microphone CM 16 (Avisoft Bioacoustics, Berlin, Germany), placed 12 cm above the floor of the sound attenuating box, and an UltraSoundGate 116H audio device (Avisoft Bioacoustics).

Techniques: