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A. Summary table presenting the proteins identified and quantified by mass spectrometry analysis of ADAM10-KO cell lysates compared to control MCF7 cells and their corresponding secreted sEVs. B-C. sEVs secreted by MCF7 cells with knock-out of ADAM10 (A10 KO) or control cells (WT) were isolated by differential ultracentrifugation of conditioned culture media. Cell lysates and sEVs were analyzed by Western blot, testing for several proteins including EphrinB3, BCAM, E-cadherin and PTK7 ( B ), SRC ( C ). Histograms represent mean signal intensities normalized to controls ± SEM, calculated from n independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, n.s. non-significant (multiple unpaired t-test).

Journal: bioRxiv

Article Title: ADAM10 tailors extracellular vesicles for content transfer rather than signaling by contact

doi: 10.64898/2026.02.12.705562

Figure Lengend Snippet: A. Summary table presenting the proteins identified and quantified by mass spectrometry analysis of ADAM10-KO cell lysates compared to control MCF7 cells and their corresponding secreted sEVs. B-C. sEVs secreted by MCF7 cells with knock-out of ADAM10 (A10 KO) or control cells (WT) were isolated by differential ultracentrifugation of conditioned culture media. Cell lysates and sEVs were analyzed by Western blot, testing for several proteins including EphrinB3, BCAM, E-cadherin and PTK7 ( B ), SRC ( C ). Histograms represent mean signal intensities normalized to controls ± SEM, calculated from n independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, n.s. non-significant (multiple unpaired t-test).

Article Snippet: Antibodies directed against SDC1 intracellular domain (D4Y7H) was from (cell signaling #12922, dilution 1/1000), GFP (A11122) from Thermofisher (dilution 1/1000), ADAM17 (abcam, #ab39162, 1/1000 or cell signaling #3976, 1/1000), ADAM10 (abcam, #ab1997, 1/1 000), GAPDH (Proteintech # 10494-1-AP), Flotillin-1 (BD Biosciences, #X11669), Ephrin B3 (santa cruz, # sc-514139), PTK7 (Proteintech, #17799), E-cadherin (R&D Systems, #AF748), BCAM (R&D Systems, #BAF148), STAT3 124H6 (cell signaling #9139), p-STAT3 (cell signaling #9131).

Techniques: Mass Spectrometry, Control, Knock-Out, Isolation, Western Blot