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Journal: JID Innovations
Article Title: Insights into Keratinocyte and Immunologic Landscape in Cutaneous Graft-Versus-Host Disease through Single-Cell Transcriptomics
doi: 10.1016/j.xjidi.2025.100373
Figure Lengend Snippet: Upregulation of inflammatory genes and downregulation of redox and regenerative genes in cGVHD epidermal keratinocyte populations. ( a ) Heatmap of top 10 most differentially expressed genes in keratinocytes in normal versus cGVHD epidermal samples. ( b ) Heatmap of top 5 most differentially expressed genes in suprabasal keratinocytes in normal versus cGVHD epidermal samples. ( c ) Heatmap of top 5 most differentially expressed genes in basal keratinocytes in normal versus cGVHD epidermal samples. Dot plot showing the percentage expressed and average expression of ( d ) alarmin genes, ( e ) inflammatory cytokines, ( f ) antioxidant genes, and ( g ) AP-1 and other transcription factor genes expressions in normal versus cGVHD samples. ( h ) Immunofluorescent staining of S100A7 and K16 in additional normal and cGVHD samples confirmed increased expressions of S100A7 and K16 in cGVHD. AP-1, activator protein-1; cGVHD, chronic graft-versus-host disease; K, keratin; redox, reduction–oxidation.
Article Snippet: A single slide for each sample was stained for 1 hour at room temperature with a pool of fluorescently conjugated antibodies against K16 (Novus Biological, catalog number NBP3-08692AF647) and
Techniques: Expressing, Staining