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Fig. 2. Effects of sc INS injection on ventrolateral ventromedial hypothalamic nucleus (VMNvl) glycogen synthase (GS) <t>protein</t> expression in V‑ or Lz‑pretreated male and estradiol‑ or oil‑Implanted OVX female rats. VMNvl tissue was bilaterally micropunch‑dissected over predetermined rostro‑caudal levels from groups of male and female rats after sc vehicle (V; sterile diluent) or INS (10.0 U neutral protamine Hagedorn insulin/kg bw) injection. Data depict mean normalized rostral ((A) male, (B) female), middle ((C) male, (D) female), and caudal ((E) male, (F) female) VMNvl GS protein <t>optical</t> <t>density</t> (OD) <t>measures</t> + S.E.M. for groups of male (left-hand column) and female (right-hand column) rats. Groups of male rats (n=4/group) were pretreated by serial daily sc injection of V‑ (gray bars) or Lz (white bars) prior to sacrifice after V injection (solid bars) or at 1.0 (diagonal‑striped bars), 3.5 (cross‑hatched bars), or 7.0 (stippled bars) h after INS injection. Groups of OVX estradiol‑ (gray bars) or oil (white bars)‑implanted female rats (n=4/group) were sacrificed at identical time points after V or INS treatment. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001.
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optical density  (Thermo Fisher)
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Fig. 2. Effects of sc INS injection on ventrolateral ventromedial hypothalamic nucleus (VMNvl) glycogen synthase (GS) <t>protein</t> expression in V‑ or Lz‑pretreated male and estradiol‑ or oil‑Implanted OVX female rats. VMNvl tissue was bilaterally micropunch‑dissected over predetermined rostro‑caudal levels from groups of male and female rats after sc vehicle (V; sterile diluent) or INS (10.0 U neutral protamine Hagedorn insulin/kg bw) injection. Data depict mean normalized rostral ((A) male, (B) female), middle ((C) male, (D) female), and caudal ((E) male, (F) female) VMNvl GS protein <t>optical</t> <t>density</t> (OD) <t>measures</t> + S.E.M. for groups of male (left-hand column) and female (right-hand column) rats. Groups of male rats (n=4/group) were pretreated by serial daily sc injection of V‑ (gray bars) or Lz (white bars) prior to sacrifice after V injection (solid bars) or at 1.0 (diagonal‑striped bars), 3.5 (cross‑hatched bars), or 7.0 (stippled bars) h after INS injection. Groups of OVX estradiol‑ (gray bars) or oil (white bars)‑implanted female rats (n=4/group) were sacrificed at identical time points after V or INS treatment. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001.
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Fig. 2. Effects of sc INS injection on ventrolateral ventromedial hypothalamic nucleus (VMNvl) glycogen synthase (GS) protein expression in V‑ or Lz‑pretreated male and estradiol‑ or oil‑Implanted OVX female rats. VMNvl tissue was bilaterally micropunch‑dissected over predetermined rostro‑caudal levels from groups of male and female rats after sc vehicle (V; sterile diluent) or INS (10.0 U neutral protamine Hagedorn insulin/kg bw) injection. Data depict mean normalized rostral ((A) male, (B) female), middle ((C) male, (D) female), and caudal ((E) male, (F) female) VMNvl GS protein optical density (OD) measures + S.E.M. for groups of male (left-hand column) and female (right-hand column) rats. Groups of male rats (n=4/group) were pretreated by serial daily sc injection of V‑ (gray bars) or Lz (white bars) prior to sacrifice after V injection (solid bars) or at 1.0 (diagonal‑striped bars), 3.5 (cross‑hatched bars), or 7.0 (stippled bars) h after INS injection. Groups of OVX estradiol‑ (gray bars) or oil (white bars)‑implanted female rats (n=4/group) were sacrificed at identical time points after V or INS treatment. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001.

Journal: Acta Neurobiologiae Experimentalis

Article Title: Hypoglycemic and post-hypoglycemic patterns of glycogen phosphorylase isoform expression in the ventrolateral ventromedial hypothalamic nucleus: impact of sex and estradiol

doi: 10.21307/ane-2021-019

Figure Lengend Snippet: Fig. 2. Effects of sc INS injection on ventrolateral ventromedial hypothalamic nucleus (VMNvl) glycogen synthase (GS) protein expression in V‑ or Lz‑pretreated male and estradiol‑ or oil‑Implanted OVX female rats. VMNvl tissue was bilaterally micropunch‑dissected over predetermined rostro‑caudal levels from groups of male and female rats after sc vehicle (V; sterile diluent) or INS (10.0 U neutral protamine Hagedorn insulin/kg bw) injection. Data depict mean normalized rostral ((A) male, (B) female), middle ((C) male, (D) female), and caudal ((E) male, (F) female) VMNvl GS protein optical density (OD) measures + S.E.M. for groups of male (left-hand column) and female (right-hand column) rats. Groups of male rats (n=4/group) were pretreated by serial daily sc injection of V‑ (gray bars) or Lz (white bars) prior to sacrifice after V injection (solid bars) or at 1.0 (diagonal‑striped bars), 3.5 (cross‑hatched bars), or 7.0 (stippled bars) h after INS injection. Groups of OVX estradiol‑ (gray bars) or oil (white bars)‑implanted female rats (n=4/group) were sacrificed at identical time points after V or INS treatment. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001.

Article Snippet: Protein band optical density (OD) measures were normalized to total in‐lane protein using Image LabTM 6.0.0 software (Bio‐Rad).

Techniques: Injection, Expressing, Sterility