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(A-D) Evaluation for mutation detection using pandemic 2009 H1N1 influenza virus genome segment 6 (neuraminidase) H275Y mutation; (E-H) Evaluation for low copy DNA detection using influenza B virus genome segment 7 (matrix protein) as a template. Upper panels (A, C, E, G) show results obtained by using Eprobes and primers designed by <t>Primer3</t> with updated tables for T M calculation of ECHOs. Lower panels (B, D, F, H) show results obtained by using Eprobes and primers designed by Edesign. Details on primers and Eprobes used are provided in and Figs.
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primer3 core c program version 2.3.4 - by Bioz Stars, 2026-06
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  Buy from Supplier
primer3 core c program version 2.3.4  (PrimerDesign Inc)
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PrimerDesign Inc primer3 core c program version 2.3.4
(A-D) Evaluation for mutation detection using pandemic 2009 H1N1 influenza virus genome segment 6 (neuraminidase) H275Y mutation; (E-H) Evaluation for low copy DNA detection using influenza B virus genome segment 7 (matrix protein) as a template. Upper panels (A, C, E, G) show results obtained by using Eprobes and primers designed by <t>Primer3</t> with updated tables for T M calculation of ECHOs. Lower panels (B, D, F, H) show results obtained by using Eprobes and primers designed by Edesign. Details on primers and Eprobes used are provided in and Figs.
Primer3 Core C Program Version 2.3.4, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primer3+core+program/pmc04749234-90-10-10?v=PrimerDesign+Inc
Average 90 stars, based on 1 article reviews
primer3 core c program version 2.3.4 - by Bioz Stars, 2026-06
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(A-D) Evaluation for mutation detection using pandemic 2009 H1N1 influenza virus genome segment 6 (neuraminidase) H275Y mutation; (E-H) Evaluation for low copy DNA detection using influenza B virus genome segment 7 (matrix protein) as a template. Upper panels (A, C, E, G) show results obtained by using Eprobes and primers designed by Primer3 with updated tables for T M calculation of ECHOs. Lower panels (B, D, F, H) show results obtained by using Eprobes and primers designed by Edesign. Details on primers and Eprobes used are provided in and Figs.

Journal: PLoS ONE

Article Title: Edesign: Primer and Enhanced Internal Probe Design Tool for Quantitative PCR Experiments and Genotyping Assays

doi: 10.1371/journal.pone.0146950

Figure Lengend Snippet: (A-D) Evaluation for mutation detection using pandemic 2009 H1N1 influenza virus genome segment 6 (neuraminidase) H275Y mutation; (E-H) Evaluation for low copy DNA detection using influenza B virus genome segment 7 (matrix protein) as a template. Upper panels (A, C, E, G) show results obtained by using Eprobes and primers designed by Primer3 with updated tables for T M calculation of ECHOs. Lower panels (B, D, F, H) show results obtained by using Eprobes and primers designed by Edesign. Details on primers and Eprobes used are provided in and Figs.

Article Snippet: Edesign is based on the Primer3 software (Primer3 core C program version 2.3.4 and Primer3 web interface version 3.0.0), for which the source code was downloaded at http://sourceforge.net/projects/primer3/files/primer3/ , and for the web interface at http://sourceforge.net/projects/primer3/files/primer3-web/ .

Techniques: Mutagenesis, Virus

(A-D) Evaluation for mutation detection using pandemic 2009 H1N1 influenza virus genome segment 6 (neuraminidase) H275Y mutation; (E-H) Evaluation for low copy DNA detection using influenza B virus genome segment 7 (matrix protein) as a template. Upper panels (A, C, E, G) show results obtained by using Eprobes and primers designed by Primer3 with updated tables for T M calculation of ECHOs. Lower panels (B, D, F, H) show results obtained by using Eprobes and primers designed by Edesign. Details on primers and Eprobes used are provided in and Figs.

Journal: PLoS ONE

Article Title: Edesign: Primer and Enhanced Internal Probe Design Tool for Quantitative PCR Experiments and Genotyping Assays

doi: 10.1371/journal.pone.0146950

Figure Lengend Snippet: (A-D) Evaluation for mutation detection using pandemic 2009 H1N1 influenza virus genome segment 6 (neuraminidase) H275Y mutation; (E-H) Evaluation for low copy DNA detection using influenza B virus genome segment 7 (matrix protein) as a template. Upper panels (A, C, E, G) show results obtained by using Eprobes and primers designed by Primer3 with updated tables for T M calculation of ECHOs. Lower panels (B, D, F, H) show results obtained by using Eprobes and primers designed by Edesign. Details on primers and Eprobes used are provided in and Figs.

Article Snippet: Our new design tool, Edesign, was developed using the established primer design software Primer3 (Primer3 core C program version 2.3.4 and Primer3 web interface version 3.0.0).

Techniques: Mutagenesis, Virus