downstream and rt primers of the stem-loop method (Sangon Biotech)
90
Structured Review
Sangon Biotech
downstream and rt primers of the stem-loop method
Downstream And Rt Primers Of The Stem Loop Method, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primer+method/stem+loop+primers/pm40327646-110-7-13
Average 90 stars, based on 1 article reviews
Downstream And Rt Primers Of The Stem Loop Method, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primer+method/stem+loop+primers/pm40327646-110-7-13
Average 90 stars, based on 1 article reviews
downstream and rt primers of the stem-loop method - by Bioz Stars,
2026-10
90/100 stars
Images
Related Articles
Synthesized:Article Title: A new candidate tumor suppressor tRF-Ser inhibits gastric cancer progression by regulating the CNBP/HSPA8 axis Article Snippet: Total RNA extraction used RNA-easy isolation reagent (Cat No. R701-01, Vazyme, China), with cDNA synthesis following the manufacturer’s protocol (Cat No. AG11745, AG, China; Cat No. R323-01, Vazyme). qRT-PCR analyses were performed with SYBR Green master mix (Cat No. Q711-02, Vazyme), using β-actin and U6 as endogenous controls for mRNA and tRF-Ser, respectively. .. The 2− ΔΔCt method was applied for quantification, with all Article Title: Excessive Erythrophagocytosis Accounts for Systemic Inflammation in Chronic Kidney Disease Article Snippet: The qPCR was performed by utilizing SYBR® Green Realtime PCR Master Mix https://doi.org/10.2147/JIR.S467136 DovePress Journal of Inflammation Research 2024:17 7114 Powered by TCPDF (www.tcpdf.org) (Toyobo Biotech Co., Ltd., Shanghai, China) and EasyPGX qPCR instrument 96 (Diatech Pharmacogenetics srl, Jesi, Ancona, Italy) under the following parameters: 95 ◦C for 2 min, 50 cycles at 95 ◦C for 10s, 60 ◦C for 30s, and 72 ◦C for 30s. .. The mRNA levels of target genes were normalized to the levels of GAPDH and calculated by the 2−ΔΔCT method and three biological replicates were analyzed for each group.18 The Article Title: Routine detection of reactive Cyfra21-1-specific CD8 + T cells and its predictive value for responsiveness to PD-1/PD-L1 inhibitor therapy in NSCLC patients. Article Snippet: Immune checkpoint inhibitors are widely used in non-small cell lung cancer (NSCLC), but with limited overall response rates.. Indicators predicting immunotherapy response are hard to routinely detect due to their invasiveness.. This study systematically screened for CD8 T-cell epitopes in Cytokeratin Fragment Antigen 21-1 (Cyfra21-1), a valuable NSCLC tumor marker, through a combination of in silico prediction, ex vivo co-cultures of peptides with patient-derived peripheral blood mononuclear cells (PBMCs), peptide competition binding assays, and peptide immunization in humanized mice. Real-time Polymerase Chain Reaction:Article Title: Excessive Erythrophagocytosis Accounts for Systemic Inflammation in Chronic Kidney Disease Article Snippet: The qPCR was performed by utilizing SYBR® Green Realtime PCR Master Mix https://doi.org/10.2147/JIR.S467136 DovePress Journal of Inflammation Research 2024:17 7114 Powered by TCPDF (www.tcpdf.org) (Toyobo Biotech Co., Ltd., Shanghai, China) and EasyPGX qPCR instrument 96 (Diatech Pharmacogenetics srl, Jesi, Ancona, Italy) under the following parameters: 95 ◦C for 2 min, 50 cycles at 95 ◦C for 10s, 60 ◦C for 30s, and 72 ◦C for 30s. .. The mRNA levels of target genes were normalized to the levels of GAPDH and calculated by the 2−ΔΔCT method and three biological replicates were analyzed for each group.18 The other:Article Title: A new candidate tumor suppressor tRF-Ser inhibits gastric cancer progression by regulating the CNBP/HSPA8 axis. Article Snippet: Small RNA sequencing (focusing on tRFs/tiRNAs) was performed for four paired GC and adjacent normal tissues by Aksomics (Shanghai, China), whereas transcriptome sequencing of tRF-Ser-overexpressing MKN-45 cells was carried out by Novogene (Beijing, China). Article Title: In-depth analysis of the treatment effect and synergistic mechanism of TanReQing injection on clinical multi-drug resistant Pseudomonas aeruginosa . Article Snippet: Total bacterial RNA was extracted following the manufacturer’s protocol (Tiangen Biotec, China), and the concentrations of RNA were measured using NanoDrop (Thermo Fisher, USA). Article Title: Perfluorohexanesulfonic Acid (PFHxS) Induces Hepatotoxicity through the PPAR Signaling Pathway in Larval Zebrafish ( Danio rerio ). Article Snippet: In recent years, the industrial substitution of longchain perand polyfluoroalkyl substances (PFAS) with short-chain alternatives has become increasingly prevalent, resulting in the widespread environmental detection of perfluorohexanesulfonic acid (PFHxS), a short-chain PFAS.. However, there remains limited information about the potential adverse effects of PFHxS at environmental concentrations to wildlife.. Here, early life stage zebrafish (Danio rerio) were exposed to environmentally relevant concentrations of PFHxS to better characterize the adverse effects of PFHxS on aquatic organisms. Control:Article Title: The Alleviating Effects of Tryptophan Metabolite Indole-3-Propionic Acid on Glycolipid Metabolism Disorders and Cognitive Dysfunction in Obese Mice Article Snippet: Obesity is a chronic metabolic condition that adversely modulate metabolism and cognitive functions.. The gut microbial derived tryptophan metabolite indole-3-propionic acid (IPA) has been identified as a potentially component in modulating metabolic perturbations and neuroprotection.. In this study, a high-fat diet (HFD)induced obese mouse model was employed to investigate the effects of IPA on obesity-induced glycolipid metabolism dysregulation and cognitive dysfunction, as well as the underlying mechanisms involved. Expressing:Article Title: The Alleviating Effects of Tryptophan Metabolite Indole-3-Propionic Acid on Glycolipid Metabolism Disorders and Cognitive Dysfunction in Obese Mice Article Snippet: Obesity is a chronic metabolic condition that adversely modulate metabolism and cognitive functions.. The gut microbial derived tryptophan metabolite indole-3-propionic acid (IPA) has been identified as a potentially component in modulating metabolic perturbations and neuroprotection.. In this study, a high-fat diet (HFD)induced obese mouse model was employed to investigate the effects of IPA on obesity-induced glycolipid metabolism dysregulation and cognitive dysfunction, as well as the underlying mechanisms involved. Polymerase Chain Reaction:Article Title: Routine detection of reactive Cyfra21-1-specific CD8 + T cells and its predictive value for responsiveness to PD-1/PD-L1 inhibitor therapy in NSCLC patients. Article Snippet: Immune checkpoint inhibitors are widely used in non-small cell lung cancer (NSCLC), but with limited overall response rates.. Indicators predicting immunotherapy response are hard to routinely detect due to their invasiveness.. This study systematically screened for CD8 T-cell epitopes in Cytokeratin Fragment Antigen 21-1 (Cyfra21-1), a valuable NSCLC tumor marker, through a combination of in silico prediction, ex vivo co-cultures of peptides with patient-derived peripheral blood mononuclear cells (PBMCs), peptide competition binding assays, and peptide immunization in humanized mice. |
