Journal: World Journal of Surgical Oncology

Article Title: Quercetin inhibits prostate cancer by attenuating cell survival and inhibiting anti-apoptotic pathways

doi: 10.1186/s12957-018-1400-z

Figure Lengend Snippet: Quercetin reduces cell viability and induces apoptosis in PCa cells. Normal prostate epithelial cells PrEC and PCa cells (LNCaP, DU-145, PC-3) were treated with quercetin, and MTT assay was used to determine cell viability ( a ). The EC 50 was calculated from the equation of the line of best fit. PCa cells were treated with 40 μM (EC 50 ) of quercetin, and vehicle-treated controls were stained with FITC-conjugated Annexin V and 7-AAD. Data was acquired by FACS and analyzed using FlowJo software ( b ). Dot plot shows percent (%) of early apoptotic (lower right quadrate) and late apoptotic cells (right upper quadrate). Apoptosis induced by quercetin (shown in top bar diagram) as well as induced necrotic cells (bottom bar diagram) were measured by paired t test to show significance compared to controls

Article Snippet: Normal prostate epithelial cells (PrEC), with materials purchased from ATCC, were cultured in basal medium with cell growth kit containing the following: 6 mM L-glutamine, 0.4% Extract P, 1.0 μM epinephrine, 0.5 ng/mL rhTGFα, 100 ng/mL hydrocortisone, 5 μg/mL rh insulin, and 5 μg/mL Apo-transferrin.

Techniques: MTT Assay, Staining, Software

Journal: Genome Biology

Article Title: epiG: statistical inference and profiling of DNA methylation from whole-genome bisulfite sequencing data

doi: 10.1186/s13059-017-1168-4

Figure Lengend Snippet: WGBS and NOMe-seq data sets

Article Snippet: PrEC cells were grown in Prostate Epithelial Cell Basal Medium (ATCC) supplemented with Prostate Epithelial Cell Growth Kit (ATCC).

Techniques: