Journal: Brain and Behavior

Article Title: Lipopolysaccharide Upregulates Neuroinflammation, Oxidative Stress Responses, and Peroxiredoxins in Depression Models

doi: 10.1002/brb3.71231

Figure Lengend Snippet: Immunohistochemistry detected the protein expression of Peroxiredoxin1 (Prdx1) and Prdx2 in brain tissues. (A) Prdx1 immunohistochemistry staining and expression levels in brain tissues; (B) Prdx2 immunohistochemistry staining and expression levels in brain tissues; ns, no significant difference, p > 0.05.

Article Snippet: The membrane was blocked with 5% skim milk in TBST for 1 h at RT, followed by incubation with primary antibodies overnight at 4°C: GAPDH (Proteintech, No. 81640‐5‐RR, China), Prdx1 (Proteintech, No. 515816‐1‐AP, China), Prdx2 (Proteintech, No. 10545‐2‐AP, China), Prdx4 (ABonal, No. 4000001442, China).

Techniques: Immunohistochemistry, Expressing, Staining

Journal: Brain and Behavior

Article Title: Lipopolysaccharide Upregulates Neuroinflammation, Oxidative Stress Responses, and Peroxiredoxins in Depression Models

doi: 10.1002/brb3.71231

Figure Lengend Snippet: RT‐qPCR detected the mRNA expression of Peroxiredoxin1 (Prdx1), Prdx2, Prdx4, and Prdx5 in brain tissues. (A) mRNA expression levels of Prdx1 in brain tissues; (B) mRNA expression levels of Prdx2 in brain tissues; (C) mRNA expression levels of Prdx4 in brain tissues; (D) mRNA expression levels of Prdx5 in brain tissues; * p < 0.05; ** p < 0.01.

Article Snippet: The membrane was blocked with 5% skim milk in TBST for 1 h at RT, followed by incubation with primary antibodies overnight at 4°C: GAPDH (Proteintech, No. 81640‐5‐RR, China), Prdx1 (Proteintech, No. 515816‐1‐AP, China), Prdx2 (Proteintech, No. 10545‐2‐AP, China), Prdx4 (ABonal, No. 4000001442, China).

Techniques: Quantitative RT-PCR, Expressing

Journal: Brain and Behavior

Article Title: Lipopolysaccharide Upregulates Neuroinflammation, Oxidative Stress Responses, and Peroxiredoxins in Depression Models

doi: 10.1002/brb3.71231

Figure Lengend Snippet: WB detected the protein expression of Peroxiredoxin1 (Prdx1), Prdx2, Prdx4, and Prdx5 in brain tissues. (A) Protein expression bands; (B) Prdx1 protein expression levels; (C) Prdx2 protein expression levels; (D) Prdx4 protein expression levels; (E) Prdx5 protein expression levels; ns, no significant difference, p > 0.05; * p < 0.05; ** p < 0.01.

Article Snippet: The membrane was blocked with 5% skim milk in TBST for 1 h at RT, followed by incubation with primary antibodies overnight at 4°C: GAPDH (Proteintech, No. 81640‐5‐RR, China), Prdx1 (Proteintech, No. 515816‐1‐AP, China), Prdx2 (Proteintech, No. 10545‐2‐AP, China), Prdx4 (ABonal, No. 4000001442, China).

Techniques: Expressing

Journal: Brain and Behavior

Article Title: Lipopolysaccharide Upregulates Neuroinflammation, Oxidative Stress Responses, and Peroxiredoxins in Depression Models

doi: 10.1002/brb3.71231

Figure Lengend Snippet: The expression levels of Prdxs, reactive oxygen species (ROS), and nitric oxide (NO) in BV2 cells. (A) ROS fluorescence images; (B) ROS fluorescence levels in BV2 cells; (C) NO levels in BV2 cell supernatant; mRNA expression levels of Prdx1 (D), Prdx2 (E), Prdx4 (F), and Prdx5 (G) in BV2 cells; ns, no significant difference, p > 0.05; * p < 0.05; **** p < 0.0001.

Article Snippet: The membrane was blocked with 5% skim milk in TBST for 1 h at RT, followed by incubation with primary antibodies overnight at 4°C: GAPDH (Proteintech, No. 81640‐5‐RR, China), Prdx1 (Proteintech, No. 515816‐1‐AP, China), Prdx2 (Proteintech, No. 10545‐2‐AP, China), Prdx4 (ABonal, No. 4000001442, China).

Techniques: Expressing, Fluorescence

Journal: Brain and Behavior

Article Title: Lipopolysaccharide Upregulates Neuroinflammation, Oxidative Stress Responses, and Peroxiredoxins in Depression Models

doi: 10.1002/brb3.71231

Figure Lengend Snippet: WB detected the protein expression of Peroxiredoxin1 (Prdx1), Prdx2, Prdx4, and Prdx5 in BV2 cells. (A) Protein expression bands; protein expression levels of Prdx1 (B), Prdx2 (C), Prdx4 (D), and Prdx5 (E) in BV2 cells; ns, no significant difference, p > 0.05; * p < 0.05.

Article Snippet: The membrane was blocked with 5% skim milk in TBST for 1 h at RT, followed by incubation with primary antibodies overnight at 4°C: GAPDH (Proteintech, No. 81640‐5‐RR, China), Prdx1 (Proteintech, No. 515816‐1‐AP, China), Prdx2 (Proteintech, No. 10545‐2‐AP, China), Prdx4 (ABonal, No. 4000001442, China).

Techniques: Expressing

Journal: Redox Biology

Article Title: MitoQ supplementation does not impact redox responses to acute exercise in skeletal muscle of older individuals

doi: 10.1016/j.redox.2025.103927

Figure Lengend Snippet: MitoQ supplementation does not impact the cellular redox state in skeletal muscle of older individuals. A and B) Skeletal muscle antioxidant protein content. C and D) Non-reducing western blotting was performed to detect dimeric and monomeric forms of PRDX2 (C) and PRDX3 (D) in skeletal muscle. Bands representing monomers (M, reduced form) and dimers (D, oxidised form) are indicated. E) 4HNE protein adduct content in skeletal muscle. ∗ Significant difference ( p < 0.05) from pre-supplementation in the same group.

Article Snippet: Proteins (15 μg protein/well) were separated by either reducing or non-reducing SDS-PAGE on 4–15 % precast stain-free gels (Bio-Rad, Hercules, CA, United States) before being transferred onto PVDF membranes (Bio-Rad, 1704156) and incubated in blocking solution and primary antibody [Total OXPHOS Antibody Cocktail (Abcam ab110413, 1:1000), Catalase (Cell Signaling Technology 14097, 1:1000), PRDX2 (Cell Signaling Technology 46855, 1:1000), PRDX3 (Abcam ab73349, 1:1000), TRX1 (Cell Signaling Technology 2298, 1:1000), TRX2 (Cell Signalling Technology 14907, 1:1000), SOD1 (Cell Signaling Technology 2770, 1:1000), SOD2 (Cell Signaling Technology 13194, 1:1000), 4HNE (Abcam ab46545, 1:500), phospho-AMPKα Thr172 (Cell Signaling Technology 2535, 1:1000), AMPKα (Cell Signaling Technology 5831, 1:1000), phospho-p38 MAPK Thr180/Tyr182 (Cell Signaling Technology 4511, 1:1000), p38 MAPK (Cell Signaling Technology 8690, 1:1000), phospho-ERK1/2 Thr202/Tyr204 (Cell Signaling Technology 9101, 1:1000), ERK1/2 (Cell Signaling Technology 9102, 1:1000).

Techniques: Western Blot

Journal: Redox Biology

Article Title: MitoQ supplementation does not impact redox responses to acute exercise in skeletal muscle of older individuals

doi: 10.1016/j.redox.2025.103927

Figure Lengend Snippet: Skeletal muscle PRDX3 is transiently oxidised during exercise and this effect is not impacted by MitoQ supplementation. A and B) PRDX2 (A) and PRDX3 (B) oxidation in skeletal muscle before (pre), immediately after (post), and 4 h after (4 h) exercise, measured using non-reducing Western blot. Bands representing monomers (M, reduced) and dimers (D, oxidised) are indicated. C) 4HNE protein adduct content in skeletal muscle before, immediately after, and 4 h after exercise.

Article Snippet: Proteins (15 μg protein/well) were separated by either reducing or non-reducing SDS-PAGE on 4–15 % precast stain-free gels (Bio-Rad, Hercules, CA, United States) before being transferred onto PVDF membranes (Bio-Rad, 1704156) and incubated in blocking solution and primary antibody [Total OXPHOS Antibody Cocktail (Abcam ab110413, 1:1000), Catalase (Cell Signaling Technology 14097, 1:1000), PRDX2 (Cell Signaling Technology 46855, 1:1000), PRDX3 (Abcam ab73349, 1:1000), TRX1 (Cell Signaling Technology 2298, 1:1000), TRX2 (Cell Signalling Technology 14907, 1:1000), SOD1 (Cell Signaling Technology 2770, 1:1000), SOD2 (Cell Signaling Technology 13194, 1:1000), 4HNE (Abcam ab46545, 1:500), phospho-AMPKα Thr172 (Cell Signaling Technology 2535, 1:1000), AMPKα (Cell Signaling Technology 5831, 1:1000), phospho-p38 MAPK Thr180/Tyr182 (Cell Signaling Technology 4511, 1:1000), p38 MAPK (Cell Signaling Technology 8690, 1:1000), phospho-ERK1/2 Thr202/Tyr204 (Cell Signaling Technology 9101, 1:1000), ERK1/2 (Cell Signaling Technology 9102, 1:1000).

Techniques: Western Blot